scholarly journals Application of the slope ratio assay technique to the estimation of thiamine activity of thiamine analogues

1969 ◽  
Vol 6 (2) ◽  
pp. 82-88 ◽  
Author(s):  
KIYOMI KOSAKA ◽  
MINORU YOSHIDA
Keyword(s):  
Slope Ratio ◽  
Thiamine Analogues ◽  
Assay Technique

Application of the slope‐ratio growth assay technique to estimate tryptophan availability in soybean meal fed to young rats

2019 ◽  
Vol 90 (9) ◽  
pp. 1270-1277 ◽  
Author(s):  
Hiroyuki Sato ◽  
Makoto Miura ◽  
Takeshi Fujieda ◽  
Masaaki Toyomizu
Keyword(s):  
Soybean Meal ◽  
Slope Ratio ◽  
Young Rats ◽  
Growth Assay ◽  
Assay Technique

Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the slope‐ratio growth assay technique in growing rats

2020 ◽  
Vol 91 (1) ◽  
Author(s):  
Hiroyuki Sato ◽  
Makoto Miura ◽  
Takeshi Fujieda ◽  
Marcin Taciak ◽  
Motoi Kikusato ◽  
...  
Keyword(s):  
Slope Ratio ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Growing Rats ◽  
Growth Assay ◽  
Assay Technique

scholarly journals Decarboxylation of Pyruvate by Thiamine Analogues

1959 ◽  
Vol 234 (4) ◽  
pp. 738-741
Author(s):  
Ralph G. Yount ◽  
David E. Metzler
Keyword(s):  
Thiamine Analogues

scholarly journals Estimation of bioavailability of DL-methionine hydroxy analogue relative to DL-methionine in layers with exponential and slope-ratio models

2004 ◽  
Vol 83 (9) ◽  
pp. 1580-1586 ◽  
Author(s):  
Z. Liu ◽  
A. Bateman ◽  
M. Bryant ◽  
A. Abebe ◽  
D. Roland
Keyword(s):  
Slope Ratio

Application of the scintillation proximity assay technique to the determination of drugs

1994 ◽  
Vol 31 (3) ◽  
pp. 103 ◽  
Author(s):  
Susan Fenwick ◽  
William N. Jenner ◽  
Paul Linacre ◽  
Regina M. Rooney ◽  
Stephen A. Wring
Keyword(s):  
Scintillation Proximity Assay ◽  
Assay Technique

Numerical Simulation for Uplift Bearing Capacity and Affecting Factors of the Digging Piles in Slope Ground

2013 ◽  
Vol 423-426 ◽  
pp. 1292-1295 ◽  
Author(s):  
Xing Yun Wang ◽  
Bin Peng ◽  
Xiao Chao Tang ◽  
Lian Fan
Keyword(s):  
Numerical Simulation ◽  
Regression Analysis ◽  
Finite Difference ◽  
Bearing Capacity ◽  
Mutual Effect ◽  
Simulation Method ◽  
Affecting Factors ◽  
Slope Ratio ◽  
Nonlinear Regression Analysis ◽  
Numerical Simulation Method

Based on the numerical simulation method, this paper has established the numerical simulation method by using of finite difference software of FLAC3D through establishing interface for digging pile-soil. It can consider mutual effect of digging pile-soil. The uplift bearing capacity of the digging pile in slope ground was calculated and the affecting factors of the bearing capacity were analyzed. The results show that the uplift bearing capacity has a negative correlation with the slope ratio, and has a positive correlation with the width or height of the foundation, which can be expressed as a quadratic polynomial. But when the slope ratio is smaller than a certain extent, the capacity no longer increases. Nonlinear regression analysis of calculation data are carried out. Finally, the calculation method of uplift bearing capacity about pile in the slope is developed, which can provide a reference to specification revision and engineering.

Latamoxef (moxalactam) kinetics in volunteers studied by a specific HPLC assay technique

1983 ◽  
Vol 12 (4) ◽  
pp. 377-386 ◽  
Author(s):  
Alexander M. M. Shepherd ◽  
Thomas C. Hardin ◽  
Thomas M. Ludden ◽  
David J. Miner ◽  
Dennis L. Coleman
Keyword(s):  
Hplc Assay ◽  
Assay Technique

Plasma Acetaldehyde: A Hew Assay Technique for Protein-Bound Acetaldehyde

1981 ◽  
Vol 61 (3) ◽  
pp. 24P-24P ◽  
Author(s):  
M. Thomas ◽  
Jenny Y.Y. Chan ◽  
F. Mansell ◽  
O.K. Lim ◽  
T.J. Peters
Keyword(s):  
Assay Technique

scholarly journals PnuT uses a facilitated diffusion mechanism for thiamine uptake

2017 ◽  
Vol 150 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Michael Jaehme ◽  
Rajkumar Singh ◽  
Alisa A. Garaeva ◽  
Ria H. Duurkens ◽  
Dirk-Jan Slotboom
Keyword(s):  
Substrate Binding ◽  
Diffusion Mechanism ◽  
Vitamin B1 ◽  
Pyridine Nucleotide ◽  
Thiamine Pyrophosphate ◽  
Facilitated Diffusion ◽  
Nicotinamide Riboside ◽  
Thiamine Transport ◽  
Shewanella Woodyi ◽  
Thiamine Analogues

Membrane transporters of the bacterial pyridine nucleotide uptake (Pnu) family mediate the uptake of various B-type vitamins. For example, the PnuT transporters have specificity for vitamin B1 (thiamine). It has been hypothesized that Pnu transporters are facilitators that allow passive transport of the vitamin substrate across the membrane. Metabolic trapping by phosphorylation would then lead to accumulation of the transported substrates in the cytoplasm. However, experimental evidence for such a transport mechanism is lacking. Here, to determine the mechanism of thiamine transport, we purify PnuTSw from Shewanella woodyi and reconstitute it in liposomes to determine substrate binding and transport properties. We show that the electrochemical gradient of thiamine solely determines the direction of transport, consistent with a facilitated diffusion mechanism. Further, PnuTSw can bind and transport thiamine as well as the thiamine analogues pyrithiamine and oxythiamine, but does not recognize the phosphorylated derivatives thiamine monophosphate and thiamine pyrophosphate as substrates, consistent with a metabolic trapping mechanism. Guided by the crystal structure of the homologous nicotinamide riboside transporter PnuC, we perform mutagenesis experiments, which reveal residues involved in substrate binding and gating. The facilitated diffusion mechanism of transport used by PnuTSw contrasts sharply with the active transport mechanisms used by other bacterial thiamine transporters.

Sign in / Sign up

Export Citation Format

Share Document