Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the slope‐ratio growth assay technique in growing rats

2020 ◽  
Vol 91 (1) ◽  
Author(s):  
Hiroyuki Sato ◽  
Makoto Miura ◽  
Takeshi Fujieda ◽  
Marcin Taciak ◽  
Motoi Kikusato ◽  
...  
2005 ◽  
Vol 4 (4) ◽  
pp. 192-196 ◽  
Author(s):  
V. Ravindran . ◽  
W.H. Hendriks . ◽  
D.V. Thomas . ◽  
P.C.H. Morel . ◽  
C.A. Butts .

2019 ◽  
Vol 90 (9) ◽  
pp. 1270-1277 ◽  
Author(s):  
Hiroyuki Sato ◽  
Makoto Miura ◽  
Takeshi Fujieda ◽  
Masaaki Toyomizu

1986 ◽  
Vol 55 (2) ◽  
pp. 441-453 ◽  
Author(s):  
E. S. Batterham ◽  
R.E. Darnell ◽  
L. S. Herbert ◽  
E. J. Major

1. The availability of lysine for pigs, rats and chicks was determined using samples of meat and bone meal (MBM) subjected to different pressure and temperature treatments during dry-rendering processing. The relation between slope-ratio estimates and three chemical tests for estimating ‘available’ lysine was assessed.2. The availability of lysine (proportion of total) for pigs was 0.97 in the control. Pressure (275 kPa gauge, 141°, for 30 min) in the early stage of rendering reduced availability to 0.74 and, in the late stage, to 0.46. Maintaining the final temperature at 125° for 4 h had little effect (0.84) whereas a higher temperature of 150° for 4 h reduced availability to 0.38.3. Availability estimates for rats were lower than those of the pig, ranging from 0.88 in the control to 0.21 for the high-temperature treatment (150° for 4 h). The effects for temperature were similar to those for the pig, whereas the effect of pressure was equally detrimental in both the early and late stages (0.45 and 0.43 respectively).4. For chicks, availability estimates were similar to those for the pig for the control (0.93) and the two temperature treatments (0.86 and 0.31 for the 125° and 150° treatments respectively). The chick was less susceptible to the effect of pressure applied to the MBM (0.78 and 0.63 for the early-and late-stage treatments respectively).5. Values for the indirect-and direct-1-fluoro-2, 4-dinitrobenzene-(FDNB)-‘available’-lysine assays decreased from 0.86 and 0.74 to 0.57 and 0.54 for the control and 150° for 4 h treatments respectively, indicating that approximately half the reduced availability involved reactions with the ε-amino group of lysine. There was little relation between the FDNB values and lysine availability for the treatments involving changes in pressure.6. There was little or no relation between dye-binding capacity of the meals, as assessed by the Acid Orange-12 dye-binding procedure (Hurrell et al. 1979), and lysine availability for the three species.


1986 ◽  
Vol 55 (2) ◽  
pp. 427-440 ◽  
Author(s):  
E. S. Batterham ◽  
R. F. Lowe ◽  
R. E. Darnell ◽  
E. J. Major

1. The availability of lysine in four meat meals (MMs), four meat and bone meals (MBMs) and two blood meals was determined using the slope-ratio assay with growing pigs, rats and chicks and with two chemical techniques.2. The availability of lysine (proportion of total) in the eight MMs or MBMs ranged from 0.48 to 0.88 for pigs, from 0.49 to 0.88 for rats and from 0.68 to 0.88 for chicks. There was no apparent relation between the availability estimates for pigs, rats and chicks for the individual meals.3. For the two blood meals, availability estimates were 1.03 and 1.13 for pigs, 0.81 and 0.80 for rats and 1.07 and 1.02 for chicks.4. Values for the indirect and direct 1-fluoro-2, 4-dinitrobenzene-‘available’-lysineassays ranged from 0.77 to 0.88 and 0.78 to 0.93 respectively for the eight MMs and MBMs. There appeared to be no relation between these values and the pig estimates.


2004 ◽  
Vol 67 (3) ◽  
pp. 550-554 ◽  
Author(s):  
LUIS MENDOZA-ROMERO ◽  
EDWARD L. C. VERKAAR ◽  
PAUL H. SAVELKOUL ◽  
ARNOLD CATSBURG ◽  
HENK J. M. AARTS ◽  
...  

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.


2016 ◽  
Vol 18 (spe) ◽  
pp. 7-12
Author(s):  
CC Pizzolante ◽  
JE Moraes ◽  
SK Kakimoto ◽  
FEL Budiño ◽  
C Móri ◽  
...  

2012 ◽  
Vol 10 (1) ◽  
pp. 169-174
Author(s):  
I Siddika ◽  
M Das ◽  
K R Sumi

A four week experiment was conducted to evaluate the effect of isoproteinous (35%) feed prepared named diet 1, 2, 3 and 4 respectively by substituting 0%, 50% , 75% and 100% fish meal with meat and bone meal in view of preparing a cost effective tilapia (Oreochromis niloticus) fry feed. The four experimental diets (diet 1 to 4) each were randomly assigned to twelve hapas with three replications. Tilapia fry (0.011g) were randomly stocked in 100 fry/9ft² hapa and fed five times a day up to their apparent satiation. The results of the study showed that the weight gain, specific growth rate (SGR), the apparent net protein utilization value and survival (%) of the fish were significantly  (P<0.05) higher and better feed utilization with lower FCR value was observed in fish fed on the diet 4 where meat and bone meal was used as sole source of protein base. There was no significant difference between the carcass moisture and lipid of fish fed with different experimental diets. The results of the study indicated that diet 1 containing fish meal (45.15% Fish meal, 27.43% Rice bran and 27.43% Wheat flour) can be replaced by meat and bone meal containing diet 4 (54.06% Meat and bone meal, 22.97% Rice bran and 22.97% Wheat flour) with no adverse effects on growth and survival of O. niloticus. Before recommendation several trials will be necessary in different farms to be  sure of the reproducibility of the result obtained in the present experiment. Cost- benefit analysis reveals that the   meat and bone meal containing diet certainly provides cheaper feed.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12110   J. Bangladesh Agril. Univ. 10(1): 169–174, 2012  


Author(s):  
P.B. Lynch ◽  
P.J.A. Sheehy

Dietary supplementation with folic acid has been shown to improve reproductive performance in sows (Lindemann 1993). However most studies have been for one cycle only and few have examined the effect of supplementation over several parities.One hundred and thirty four crossbred sows ranging in parity from 2 to 4 were selected at farrowing and randomly allocated to two dietary treatments of low and high supplemental folic acid (0 and 10 g per tonne, Roche Products Ltd.). Treatments were applied for the following three lactations and post weaning periods, two full pregnancies and to day 30 of the pregnancy following the third lactation. The diet fed contained barley, wheat, soyabean meal and meat and bone meal with nutrient levels of 14.0 MJ DE/kg and 1.02% lysine. Sows were individually penned throughout with restricted feeding in pregnancy (2.2 kg/day increasing to 2.5 kg/day in the final month), and ad libitum in lactation (approx 5.0 kg/day) and post weaning (approx 3.4 kg/day). Blood samples for determination of plasma and red cell folate were taken from 14 sows per treatment on days 4, 50 and 110 of one cycle. These were determined by a microbiological assay (modification of methods of Scott et al 1974 and Wilson and Home 1982).


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