Dual Inoculation With Arbuscular Mycorrhizal Fungi and Phosphorus Solubilizing Fungi Synergistically Enhances the Mobilization and Plant Uptake of Phosphorus From Meat and Bone Meal

Phosphorus (P) is the second most important mineral nutrient for plant growth and plays a vital role in maintaining global food security. The natural phosphorus reserves [phosphate rock (PR)] are declining at an unprecedented rate, which will threaten the sustainable food supply in near future. Rendered animal byproducts such as meat and bone meal (MBM), could serve as a sustainable alternative to meet crop phosphorus demand. Even though nitrogen (N) from MBM is readily mineralized within a few days, >75% of the P in MBM is present as calcium phosphate that is sparingly available to plants. Thus, application of MBM with the aim of meeting crop N demand could result in buildup of P reserves in soil, which necessitates the need to improve the P mobilization from MBM to achieve higher plant P use efficiency. Here, we tested the potential of two microbial inoculum-arbuscular mycorrhizal fungi (AMF) and P solubilizing fungi (Penicillium bilaiae), in improving the mobilization of P from MBM and the subsequent P uptake by maize (Zea mays). Compared to the non-inoculated MBM control, the application of P. bilaiae increased the P mobilization from MBM by more than two-fold and decreased the content of calcium bound P in the soil by 26%. However, despite this mobilization, P. bilaiae did not increase the tissue content of P in maize. On the other hand, AMF inoculation with MBM increased the plant root, shoot biomass, and plant P uptake as compared to non-inoculated control, but did not decrease the calcium bound P fraction of the soil, indicating there was limited P mobilization. The simultaneous application of both AMF and P. bilaiae in association with MBM resulted in the highest tissue P uptake of maize with a concomitant decrease in the calcium bound P in the soil, indicating the complementary functional traits of AMF and P. bilaiae in plant P nutrition from MBM. Arbuscular mycorrhizal fungi inoculation with MBM also increased the plant photosynthesis rate (27%) and root phosphomonoesterase activity (40%), which signifies the AMF associated regulation of plant physiology. Collectively, our results demonstrate that P mobilization and uptake efficiency from MBM could be improved with the combined use of arbuscular mycorrhizal fungi and P. bilaiae.

The Effect of Meat and Bone Meal (MBM) on Crop Yields, Nitrogen Content and Uptake, and Soil Mineral Nitrogen Balance

A long-term (six year) field experiment was conducted in Poland to evaluate the effect of meat and bone meal (MBM), applied without or with mineral nitrogen (N) fertilizer, on crop yields, N content and uptake by plants, and soil mineral N balance. Five treatments were compared: MBM applied at 1.0, 1.5, and 2.0 Mg ha−1, inorganic NPK, and zero-fert check. Mineral N accounted for 100% of the total N rate (158 kg ha−1) in the NPK treatment and 50%, 25%, and 0% in MBM treatments. The yield of silage maize supplied with MBM was comparable with that of plants fertilized with NPK at 74 Mg ha−1 herbage (30% DM) over two years on average. The yields of winter wheat and winter oilseed rape were highest in the NPK treatment (8.9 Mg ha−1 grain and 3.14 Mg ha−1 seeds on average). The addition of 25% and 50% of mineral N to MBM had no influence on the yields of the tested crops. The N content of plants fertilized with MBM was satisfactory (higher than in the zero-fert treatment), and considerable differences were found between years of the study within crop species. Soil mineral N content was determined by N uptake by plants rather than the proportion of mineral N in the total N rate. Nitrogen utilization by plants was highest in the NPK treatment (58%) and in the treatment where mineral N accounted for 50% of the total N rate (48%).

Rate and Timing of Meat and Bone Meal Applications Influence Growth, Yield, and Soil Water Nitrate Concentrations in Sweet Corn Production

Using local resources and minimizing environmental impacts are two important components of sustainable agriculture. Meat and bone meal (MBM), tankage, is a locally produced organic fertilizer. This study was conducted to investigate the response of sweet corn (Zea mays L. var. saccharata Stuart.) and soil water nitrate (NO3-N) concentration to MBM application at two locations, Waimānalo and Poamoho, on the island of O’ahu. The objectives were to determine effects of six application rates (0, 112, 224, 336, 448 and 672 kg N ha−1) and two application timings (preplant and split application) on: (1) sweet corn growth, yield, and quality, and (2) soil water nitrate concentration within and below the root zone. The split-plot was designed as four replicates randomly arranged in a complete block. Plant growth of roots and shoots, yield, and relative leaf chlorophyll content of sweet corn increased with increasing application rates of MBM in both locations. At Poamoho, yield was 13.6% greater in preplant versus split application. Nitrate-nitrogen losses were reduced by 20% at Waimānalo and 40% at Poamoho when MBM was applied in split applications. These findings suggest that MBM is an effective nitrogen source for sweet corn and a split application of MBM may reduce the potential for pollution.

Application of Granular and Non-Granular Organic Fertilizers in Terms of Energy, Environmental and Economic Efficiency

Granular organic fertilizers have been increasingly used in agriculture due to the longer delivery of nutrients to plants and the milder impact on the environment. The aim of this study was to determine the energy, environmental and economic efficiency of granular and non-granular organic fertilizers. Four technological scenarios of organic fertilizer use were used for comparative assessment: (1) manure fertilization (16.0 t ha−1), (2) manure fertilization (30.0 t ha−1), (3) manure pellet fertilization (2.0 t ha−1), and (4) fertilization with meat and bone meal pellets (0.7 t ha−1). Experimental studies using the mass flow method of laser spectroscopy were performed to evaluate the comparative environmental impact of granular and non-granular organic fertilizers. Economic assessment was performed for mechanized technological operations of loading, transportation and distribution of organic fertilizers, estimating the price of aggregates used and fuel consumed, the costs of individual technological operations and other indirect costs. The results showed that for mechanized technological operations, when fertilizing with granular organic manure and meat and bone meal fertilizer, energy consumption is 3.2 to 4.0 times lower compared to fertilization with manure. The average ammonia (NH3) emissions from granular organic fertilizers were found to be six times lower than from non-granular organic fertilizers. The lowest costs for mechanized works were incurred when using meat and bone meal pellets, the highest economic benefits of organic fertilizers by elements was when using manure 30 t ha−1, and the highest costs for organic fertilizers were incurred when using manure pellets.

The Microscopic Detection of Animal Proteins in Animal Feed Regarding Bovine Spongiform Encephalopathy

Due to the actuality of spongiform encephalopathies and their proven spreading by means of animal feed containing meat and bone meal, the description and measurement of osteocytic lacunae contributes to more easily distinguish bone fragments in meat and bone meal. Transmissible spongiform encephalopathies (TSEs) have attracted a lot of attention, especially after 1986, when the first case of BSE (bovine spongiform encephalopathy) was detected. Since the outbreak of spongiform encephalopathy (BSE), the use of animal protein including bone meal as an ingredient in animal feed has been controlled by several regulations including Regulation (EC) 999/2001, Regulation (EC) 1774/2002, and Regulation (EC) 1234/2003. The classical microscopic method is the only official method for detecting animal protein in animal feed in the European Union (Commission Regulation (EC) 152/2009). By applying the microscopic method to the animal feed samples, we performed detection in order to determine the presence of animal proteins that originate from mammals and fish. The microscopic analysis of all 421 samples, of which 115 were raw materials for the production of animal feed, 230 were concentrates for ruminant nutrition and 76 were concentrates for non-ruminant nutrition (32 concentrates for laying hens and 44 concentrates for pigs), did not provide positive results, that is, no remains of animal tissues of mammalian origin were found in any specimen. Whereas in 10 out of 32 (31.25%) concentrates intended for non-ruminant nutrition (laying hens), pieces of fish tissue were found. In these samples, we usually detected the presence of fish bones, gills and scales.

Mineral digestibility of different animal sources for the silver catfish Rhamdia voulezi

This study aimed to evaluate the apparent digestibility coefficients (ADCs) of dry matter, crude protein, gross energy, and minerals of marine fish meal (MFM), salmon meal (SM), tilapia by-product meal (TBM), meat and bone meal (MBM), poultry by-product meal (PBM), blood meal (BM), and feather meal (FM) by silver catfish Rhamdia voulezi. Groups of 12 fish were fed the experimental diet three times a day until apparent satiation, and the fecal samples were collected from an accumulation device. SM and PBM exhibited the highest digestibility values for dry matter, crude protein, and gross energy, while MBM and FM presented significantly lower ADCs for dry matter. BM and FM exhibited higher ADCs for phosphorus, 65.05 and 63.87%, respectively. The ADCs for calcium were 58.8% for MFM, 56.69% for TBM, and 60.08% for PBM. PBM and FM had the highest iron ADCs, 44.01 and 46.29%, respectively. Magnesium ADCs ranged from 44.87% for MBM to 75.50% for TBM. BM had the highest digestibility for zinc (62.77%), whereas MBM (36.68%) and FM (39.39%) had the lowest. In general, SM and PBM showed higher values as feedstuffs for silver catfish feeds. At the same time, the digestibility was lower for TBM and MBM for macronutrients and minerals such as phosphorus, iron, and zinc.

Energetic values of animal by-products for broiler chickens of different ages

The objective of this study was to determine the chemical composition and apparent metabolizable energy (AME), AME corrected for nitrogen balance (AMEn) and its respective metabolizable coefficients of animal byproducts for broiler chickens with different ages. Meat and bone meal, poultry by-product meal, tilapia processing residue and poultry fat were evaluated. A total of 760 male broiler chickens were used and evaluated and the phases: pre-starter (1 to 8 d of age); starter (11 to18 d); grower 1 (21 to 28 d); grower 2 (31 to 38 d), and finisher (41 to 48 d). Total excreta collection method was performed in five metabolism assays. The experimental design was completely randomized, and data were submitted to analysis of variance, posteriorly, the four feeds were compared by Tukey test and a regression analysis was performed with broiler chickens age. The significance was considered at 5% probability. The values of AME and AMEn were higher in older birds for all ingredients. The lesser CAME and CAMEn were obtained for meat and bone meal, for the regression analysis poultry by-product meal, tilapia processing residue and poultry fat had an increased linear effect with birds age and there was no adjust for meat and bone meal for regression analysis.