Latamoxef (moxalactam) kinetics in volunteers studied by a specific HPLC assay technique

1983 ◽  
Vol 12 (4) ◽  
pp. 377-386 ◽  
Author(s):  
Alexander M. M. Shepherd ◽  
Thomas C. Hardin ◽  
Thomas M. Ludden ◽  
David J. Miner ◽  
Dennis L. Coleman
Keyword(s):  
Hplc Assay ◽  
Assay Technique

A new simple HPLC assay for the quantification of ertapenem in human plasma, lung tissue, and broncho-alveolar lavage fluid

2006 ◽  
Vol 832 (2) ◽  
pp. 231-235 ◽  
Author(s):  
R MUNDKOWSKI ◽  
J MAJCHERPESZYNSKA ◽  
O BURKHARDT ◽  
T WELTE ◽  
B DREWELOW
Keyword(s):  
Human Plasma ◽  
Lung Tissue ◽  
Lavage Fluid ◽  
Hplc Assay

Application of the scintillation proximity assay technique to the determination of drugs

1994 ◽  
Vol 31 (3) ◽  
pp. 103 ◽  
Author(s):  
Susan Fenwick ◽  
William N. Jenner ◽  
Paul Linacre ◽  
Regina M. Rooney ◽  
Stephen A. Wring
Keyword(s):  
Scintillation Proximity Assay ◽  
Assay Technique

scholarly journals A sensitive HPLC-FLD method for the quantification of 6-O-demethylmenisporphine isolated from Menispermi Rhizoma in rat plasma

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jinxia Wei ◽  
Jia Shao ◽  
Yanan Li ◽  
Yubo Li
Keyword(s):  
Mobile Phase ◽  
Internal Standard ◽  
Rat Plasma ◽  
Hplc Assay ◽  
Isocratic Elution ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
The Mean ◽  
Preclinical Safety ◽  
Concentration Levels

Abstract Background To investigate the pharmacokinetics of 6-O-demethylmenisporphine, an oxoisoaporphine alkaloid with significant anti-tumor activities and isolated from Menispermi Rhizoma, a novel and sensitive HPLC assay was established for 6-O-demethylmenisporphine quantification in rat plasma. Methods Peak responses were detected by a highly selective and sensitive fluorescence detector with 426-nm excitation and 514-nm emission wavelengths. Curcumin was employed as the internal standard (IS). A Capcell Pak C18 column (150 mm × 4.6 mm i.d., 5 μm) and an isocratic elution procedure with a flow rate of 1.0 mL/min were used to exclude the endogenous interfering substance. Acetonitrile-water (68:32, v/v) containing 1% formic acid was employed as mobile phase. A 7-point calibration curve that covered the concentration range of 10–2500 ng/mL was constructed. Results A good linearity was observed with a correlation coefficient (r) of 0.9993. The lower limit of quantification for 6-O-demethylmenisporphine was 10 ng/mL. The mean recoveries of analyte in rat plasma exceeded 80.5%. The precision at four concentration levels was within 11.3% and the accuracy ranged from − 7.6 to 6.7%. Conclusion Using this new HPLC-FLD method, the investigation of plasma samples from rats following oral dosing of neat compound and Menispermi Rhizoma extract was successfully conducted. The results will provide a reference for the evaluation of preclinical safety of 6-O-demethylmenisporphine.

scholarly journals A Sensitive High-Throughput HPLC Assay for Simultaneous Determination of Everolimus and Clobetasol Propionate

2008 ◽  
Vol 46 (1) ◽  
pp. 23-29 ◽  
Author(s):  
K. Marika ◽  
F. Katherine ◽  
L. Jianmin ◽  
C. G. Mike ◽  
F. Debra
Keyword(s):  
Simultaneous Determination ◽  
High Throughput ◽  
Clobetasol Propionate ◽  
Hplc Assay

Plasma Acetaldehyde: A Hew Assay Technique for Protein-Bound Acetaldehyde

1981 ◽  
Vol 61 (3) ◽  
pp. 24P-24P ◽  
Author(s):  
M. Thomas ◽  
Jenny Y.Y. Chan ◽  
F. Mansell ◽  
O.K. Lim ◽  
T.J. Peters
Keyword(s):  
Assay Technique

scholarly journals Incorporation of Fluorescence Ceramide-Based HPLC Assay for Rapidly and Efficiently Assessing Glucosylceramide Synthase In Vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sachin K. Khiste ◽  
Salman B. Hosain ◽  
Yixuan Dong ◽  
Mohammad B. Uddin ◽  
Kartik R. Roy ◽  
...  
Keyword(s):  
Hplc Assay ◽  
Glucosylceramide Synthase
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