Prevention of Mammary Tumor Development through Neuroimmunomodulation in the Spleen and Lymph Nodes of Old Female Sprague-Dawley Rats by L-Deprenyl

2013 ◽  
Vol 20 (3) ◽  
pp. 141-151 ◽  
Author(s):  
Srinivasan ThyagaRajan ◽  
Lily Tran ◽  
Christine A. Molinaro ◽  
Daila S. Gridley ◽  
David L. Felten ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mammary Tumor ◽  
Tumor Development ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Spleen And Lymph Nodes

scholarly journals Potency of Murraya koenigii Leaves as Anti-Cancer Mammary in 7,12 dimethylbenz(α) anthracene (DMBA) induced-Sprague Dawley Rats

2020 ◽  
Vol 151 ◽  
pp. 01058
Author(s):  
Siti Aisyah ◽  
Ekowati Handharyani ◽  
Nurliani Bermawie ◽  
Agus Setiyono
Keyword(s):  
Mammary Tumor ◽  
Tumor Size ◽  
Histopathological Examination ◽  
Tumor Development ◽  
Collagen Fibers ◽  
Female Rats ◽  
Sprague Dawley ◽  
Murraya Koenigii ◽  
Sprague Dawley Rat ◽  
Sprague Dawley Rats

The purpose of the research was to study the potency of Murraya koenigii leaves extract to overcome the mammary tumor in Sprague Dawley rat. Thirty-five female rats were divided into seven groups: control (P1), tumor without therapy (P2), methotrexate group (P3), curative groups (P4 and P5) were given extract after the tumor was formed, and preventive groups (P6 and P7) were given extract before the tumor was formed with dose of 300 and 400 mg/kg, respectively. The induction of mammary tumor in rats were carried out using 7,12 dimethylbenz(α) anthracene (DMBA) subcutaneously. Bodyweight and tumor size were measured every week for 4 weeks. At the end of treatment, rats were euthanized and mammary glands were collected for histopathological examination. The result showed tumor size in P2 was significantly higher (p<0.05) than in other groups. On the other hand, tumor size in P4 and P6 were significantly smaller (p<0.05) compared to P5 and P7. Histopathological changes showed PMN cells, 1-3 layers of cuboid epithelial and solid collagen fibers proliferation in P2, while in P3 to P7 showed moderate collagen fibers proliferation. In conclusion, the administration of the extract at a dose of 300 mg/kg can decelerate tumor development in Sprague Dawley rat mammary gland.

Lymphocyte Subset Analyses in Blood, Spleen and Lymph Nodes of Female Sprague-Dawley Rats after Short or Prolonged Exposure to a 50 Hz 100-μT Magnetic Field

1999 ◽  
Vol 152 (4) ◽  
pp. 436 ◽  
Author(s):  
Susanne Thun-Battersby ◽  
Jürgen Westermann ◽  
Wolfgang Löscher ◽  
Jurgen Westermann ◽  
Wolfgang Loscher
Keyword(s):  
Magnetic Field ◽  
Lymph Nodes ◽  
Prolonged Exposure ◽  
Lymphocyte Subset ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Spleen And Lymph Nodes

Dose-Rate Effects for Mammary Tumor Development in Female Sprague-Dawley Rats Exposed to X and γ Radiation

1989 ◽  
Vol 118 (3) ◽  
pp. 545 ◽  
Author(s):  
J. R. Johnson ◽  
N. J. Gragtmans ◽  
D. K. Myers ◽  
A. R. Jones
Keyword(s):  
Dose Rate ◽  
Mammary Tumor ◽  
Tumor Development ◽  
Sprague Dawley ◽  
Γ Radiation ◽  
Dose Rate Effects ◽  
Sprague Dawley Rats ◽  
Rate Effects

Rasam (South Indian Spice Soup) - Attenuates the Mammary Tumor Induction Magnitude of 7,12-Dimethylbenz[a] anthracene in Sprague–Dawley Rats

2020 ◽  
Vol 16 (5) ◽  
pp. 467
Author(s):  
MuthuKumaradoss Mohanmarugaraja ◽  
Agilandeswari Devarajan ◽  
MuthuKumaradoss Kathiravan
Keyword(s):  
Mammary Tumor ◽  
Sprague Dawley ◽  
Tumor Induction ◽  
South Indian ◽  
Sprague Dawley Rats

scholarly journals AN ELECTRON MICROSCOPE STUDY OF LYMPHATIC TISSUE IN RUNT DISEASE

1965 ◽  
Vol 25 (3) ◽  
pp. 149-177 ◽  
Author(s):  
Leon Weiss ◽  
Alan C. Aisenberg
Keyword(s):  
Lymph Nodes ◽  
Plasma Cells ◽  
Cell Types ◽  
Sprague Dawley ◽  
Connective Tissues ◽  
Splenic Cells ◽  
Reticular Cells ◽  
Spleen And Lymph Nodes ◽  
Myelin Figure ◽  
Rats And Mice

The thymus, spleen, and lymph nodes were studied in runt disease induced by a graft of intravenously injected homologous splenic cells into newborn rats and mice. Adult Long-Evans cells (70 x 106) were injected into Sprague-Dawley rats. Adult DBA cells (7 x 106) were injected into C57BL/6 mice. Runted rats were sacrificed at 14 to 28 days of age; mice at 10 to 20 days. The thymic cortex is depleted of small lymphocytes. Those remaining are severely damaged and phagocytized. Evidence of damage includes swelling of mitochondria, myelin figure formation, margination of chromatin, and sharp angulation in nuclear contour. Large numbers of macrophages are present. Epithelial-reticular cells which envelop small cortical blood vessels are often retracted, with the result that the most peripheral layer in the thymic-blood barrier suffers abnormally large gaps. Lymphocytes of the periarterial lymphatic sheaths of spleen and of the cortex of lymph nodes are reduced in number and damaged. Vast numbers of plasma cells and many lymphocytes are evident throughout lymph nodes, in the periarterial lymphatic sheaths, and in the marginal zone and red pulp of the spleen. Plasma cells are of different sizes, the larger having dilated sacs of endoplasmic reticulum. Lymphocytes are small to medium in size. They contain, in varying quantity, ribosomes and smooth membrane-bounded cytoplasmic vesicles approximately 350 to 500 A in diameter. Most plasma cells and lymphocytes are damaged and many of these are phagocytized. Many lymphocytes in lymph nodes, however, show no evidence of damage. Reticular cells and other fixed cells of the connective tissues seldom appear affected. Thus, the major cell types reacting in runt disease are lymphocytes, plasma cells, and histiocytes or macrophages. It appears, therefore, that both the delayed and immediate types of sensitivity play a part in this disease.

scholarly journals Effect of Vitex agnus Extract on MNU Induced Mammary Tumor of Sprague Dawley Rats

2017 ◽  
Vol 9 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Maninder Kour ◽  
Vinodini Nithyananda Madom Anantharaya ◽  
Kumar Megur Ramakrishna Bhat ◽  
Shrijeet Chakraborti ◽  
Bhagyalakshmi Kodavanji
Keyword(s):  
Mammary Tumor ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Quantitation of Peroxisome Proliferation using Silver Enhanced Immunogold Labeling on Glycol Methacrylate (GMA) Sections

1998 ◽  
Vol 4 (S2) ◽  
pp. 1094-1095
Author(s):  
G.D. Gagne ◽  
A.H. Illi ◽  
D. Hickman ◽  
J.A. Fagerland
Keyword(s):  
Protein A ◽  
Tumor Development ◽  
Acrylic Resin ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferation ◽  
Sprague Dawley ◽  
Peroxisomal Enzyme ◽  
Glycol Methacrylate ◽  
Sprague Dawley Rats ◽  
Safety Studies

Assessment of peroxisome proliferation is important in drug safety studies, since peroxisome proliferation often precedes tumor development in rodents. Proliferation can be measured morphometrically on sections by immunostaining for the peroxisomal enzyme catalase. Previous studies have used tissue embedded in acrylic resins such as LR White, which requires tissue sizes of 1-2 mm. Glycol methacrylate (GMA) is an acrylic resin that preserves antigenicity in much larger pieces of tissue. In this study we applied immunogold-silver staining (IGSS) to GMA sections for the quantitation of peroxisome proliferation in rat livers.Sprague-Dawley rats were given clofibrate, a known peroxisome proliferator, for 14 days. Livers were removed, fixed in neutral buffered formalin for 4 hours, and embedded in GMA. Sections (3 μm thick) were incubated with antibody to catalase followed by protein A-gold (PAG), and the gold label was amplified using a commercial silver enhancement kit. Tissue sections were counterstained with propidium iodide (PI).

scholarly journals Efficacy of Vitex agnus in lowering prolactin level in mammary tumor induced SD rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 380-386
Author(s):  
Maninder Kour ◽  
Kumar Megur Ramakrishna Bhat ◽  
Vinodini Nithyanandamadom Anatharaya ◽  
Shrijeet Chakraborty ◽  
Reshma Kumara Chandra
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Mammary Tumor ◽  
Tumor Regression ◽  
Tumor Development ◽  
Female Rats ◽  
Benign Tumors ◽  
Sprague Dawley ◽  
Sd Rats ◽  
Foamy Macrophages

Introduction and Aim: Breast cancer accounts for about 30% of all cancers in women. The present study aims to see the role of prolactin and Vitex agnus fruit extract in breast cancer progression in mammary tumor induced Sprague Dawley (SD) rats. Materials and Methods: Thirty-day old inbred SD female rats of body weight 70-80 grams were taken for this study. The rats were induced with N- Methyl-Nitroso-Urea for mammary tumor development. After the development of palpable and visible tumor the rats were treated with anti-prolactin drug (Cabergoline) and a prolactin lowering herb Vitex agnus Castus (VAC) for two months. After the treatment the rats were sacrificed for antioxidants estimation and histopathological section examination. Results: The rats treated with anti-prolactin drug showed benign tumors with hyperplasia and lactational change proving the presence of prolactin in the tumor tissue, whereas the plant extract showed mammary tumor regression by the presence of foamy macrophages in the histopathological sections. Results also showed treatment with cabergoline increased the GSH level and decreased the MDA level compared to tumor induced group. Conclusion: Prolactin may have a potential role in progression of breast cancer and Vitex agnus extract showed a prolactin lowering effect and facilitated in regression of the tumor.  

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