Long-term storage of bradyrhizobia in sterile water

1993 ◽  
Vol 39 (10) ◽  
pp. 998-1001 ◽  
Author(s):  
Stephen C. Wagner ◽  
Horace D. Skipper
Keyword(s):  
Antibiotic Resistance ◽  
Bradyrhizobium Japonicum ◽  
Sterile Water ◽  
Simple Method ◽  
Antibiotic Resistant ◽  
Long Term Storage ◽  
Yeast Extract Mannitol ◽  
Resistant Strains ◽  
Term Storage

Preservation of strains of bradyrhizobia is an important aspect of legume research and development. Storage of strains of bradyrhizobia in sterile water is an inexpensive and simple method of preservation. This study was conducted to determine if bradyrhizobia strains remained viable after being stored in water for 66 months. Twenty-three bradyrhizobia strains were viable and pure after 66 months of storage in water. Nine Bradyrhizobium japonicum strains that were resistant to antibiotics remained viable and pure after 60 months of storage in water. However, three of the nine antibiotic-resistant strains lost antibiotic resistance after storage as assessed by failure to grow on yeast extract mannitol agar plates containing antibiotics. Maintenance in water should be limited to 48 months, at which time bradyrhizobia strains can be revived in media containing appropriate antibiotics and resuspended in water. These results demonstrated that bradyrhizobia strains can remain viable for at least 66 months of storage in water, but these bacteria may lose traits such as antibiotic resistance if stored too long in sterile water.Key words: bradyrhizobia, storage, water.

Changes in the physiological and agricultural characteristics of peat-based Bradyrhizobium japonicum inoculants after long-term storage

2000 ◽  
Vol 54 (2) ◽  
pp. 206-211 ◽  
Author(s):  
C. Revellin ◽  
G. Meunier ◽  
J. J. Giraud ◽  
G. Sommer ◽  
P. Wadoux ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Long Term Storage ◽  
Term Storage

Revival of saprotrophic and mycorrhizal basidiomycete cultures from cold storage in sterile water

1989 ◽  
Vol 35 (11) ◽  
pp. 1055-1060 ◽  
Author(s):  
Dana L. Richter ◽  
Johann N. Bruhn
Keyword(s):  
Mycorrhizal Fungi ◽  
Room Temperature ◽  
Laccaria Bicolor ◽  
Sterile Water ◽  
Saprotrophic Fungi ◽  
Long Term Storage ◽  
Culture Viability ◽  
Culture Maintenance ◽  
Term Storage

Vegetatively colonized agar cores of 135 basidiomycete isolates, representing 83 species in 38 genera, were stored at 5 °C in tubes of sterile distilled water. Viability, based on growth at room temperature, was determined after storage periods ranging from 3 to 48 months. Thirty-seven isolates of saprotrophic fungi represented 32 species of 22 genera; 98 isolates of mycorrhizal fungi represented 51 species of 16 genera. Most isolates of saprotrophic species were viable, even after the most extended periods of storage. Many isolates of mycorrhizal species demonstrated lower viability, especially over longer periods of storage. Among our isolates of mycorrhizal fungi, Laccaria bicolor (seven isolates) and Laccaria laccata (nine isolates) appeared well suited to this method of long-term storage, whereas Scleroderma species (22 isolates) did not.Key words: culture maintenance, culture viability, fungal preservation, long-term storage, vegetative cultures.

Survival of wood-inhabiting fungi stored for 10 years in water and under oil

1992 ◽  
Vol 38 (8) ◽  
pp. 861-864 ◽  
Author(s):  
Gary C. Johnson ◽  
Andrew K. Martin
Keyword(s):  
Key Words ◽  
Water Storage ◽  
Mineral Oil ◽  
Sterile Water ◽  
Long Term Storage ◽  
Oil Storage ◽  
Term Storage ◽  
Water Survival

Subcultures of 1064 isolates of 393 species of wood-inhabiting fungi were stored both under sterile mineral oil and in sterile water for 10 years. A high proportion (98%) of the Basidiomycotina isolates survived storage under oil, whereas only 26% survived in sterile water. Survival of most isolates of Ascomycotina (91% under oil, 72% in water) and Deuteromycotina (96% under oil, 92% in water) was somewhat less in water than under oil. Key words: fungal storage, long-term storage, oil storage, water storage, Basidiomycotina.

scholarly journals THE EFFECT OF STORAGE METHODS ON VIABILITY AND SYMBIOTIC PROPERTIES OF SOYBEAN NODULE BACTERIA

2014 ◽  
Vol 20 ◽  
pp. 15-22
Author(s):  
T. M. Kovalevska ◽  
D. V. Krutylo ◽  
V. P. Horban ◽  
I. M. Romanova ◽  
T. O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Partial Restoration ◽  
Long Term Storage ◽  
Soybean Nodule ◽  
Storage Methods ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Term Storage

The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.

Highly efficient long-term storage of carrier-bound anammox biomass

2016 ◽  
Vol 74 (8) ◽  
pp. 1911-1918
Author(s):  
Steffen Engelbrecht ◽  
Mbengamina Terence Fondengcap ◽  
Kristina Rathsack ◽  
Marion Martienssen
Keyword(s):  
Specific Activity ◽  
Anammox Bacteria ◽  
Simple Method ◽  
Long Term Storage ◽  
Generation Times ◽  
Anammox Activity ◽  
Potential Alternative ◽  
Redox Buffer ◽  
Term Storage

The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 °C, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.

scholarly journals Improved lyophilization conditions for long-term storage of bacteriophages

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Prasanth Manohar ◽  
Nachimuthu Ramesh
Keyword(s):  
Antibiotic Resistance ◽  
Polyethylene Glycol ◽  
Phage Therapy ◽  
Pharmaceutical Products ◽  
Long Term Storage ◽  
Pharmaceutical Applications ◽  
The Stability ◽  
Term Storage

Abstract Phage therapy is one of the promising alternatives to combat the increasing problem of antibiotic resistance. Lyophilization is used for the preparation of pharmaceutical products to improve their stability in long-term storage. The aim of this study was to improve the stability of lyophilized bacteriophages using different excipients. Three lytic bacteriophages Escherichia phage ECP311, Klebsiella phage KPP235 and Enterobacter phage ELP140 were subjected to lyophilization using six different excipients: glucose, sucrose, gelatin, mannitol, polyethylene glycol and sorbitol. The lyophilized phages were stored at 4 °C and 37 °C and rehydrated using biological saline to test their viability at 5 months interval up to 20 months. The results showed that the use of sucrose, gelatin and their combination was beneficial in maintaining the viability of phages post-lyophilization. When lyophilized phages were stored at 4 °C, their viability was maintained up to 20 months, but at 37 °C there was a reduction in activity after 10 months. This is one of the few studies to report the lyophilization of phage cocktails to have viability for up to 10 months. Our study identified promising lyophilization excipients to effectively lyophilize bacteriophages for pharmaceutical applications and long-term storage.

scholarly journals Effect of long-term storage on induced photon emission of boar spermatozoa

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 61-65 ◽  
Author(s):  
P. Gogol ◽  
B. Szczęśniak-Fabiańczyk
Keyword(s):  
Oxidative Damage ◽  
Sperm Motility ◽  
Photon Emission ◽  
Stress Factors ◽  
Simple Method ◽  
Long Term Storage ◽  
Boar Spermatozoa ◽  
Term Storage

Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was investigated using a luminometer during a 12-day storage at 15°C in Biosolwens extender. It was shown that the day of storage had a significant effect on some photon emission parameters. During storage, a significant increase in the Integral, Peak max. and Slope max. parameters and a decline in the T.-half (fall) parameter were observed. A significant correlation was observed between photon emission parameters and sperm motility. In conclusion, the results of the present study indicate that the measurement of induced photon emission can be an alternative, sensitive and relatively simple method for assessing the effect of preservation on oxidative damage to boar spermatozoa.  

A simple method for long-term storage of Acanthamoeba species

2008 ◽  
Vol 104 (4) ◽  
pp. 935-937 ◽  
Author(s):  
Diana Axelsson-Olsson ◽  
Jenny Olofsson ◽  
Patrik Ellström ◽  
Jonas Waldenström ◽  
Björn Olsen
Keyword(s):  
Simple Method ◽  
Long Term Storage ◽  
Acanthamoeba Species ◽  
Term Storage
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