A Simple Method of Lyophilization for the Long-Term Storage of Slime Molds and Small Soil Amoebae

BioScience ◽  
1965 ◽  
Vol 15 (7) ◽  
pp. 469-469 ◽  
Author(s):  
Norman S. Kerr
Keyword(s):  
Slime Molds ◽  
Simple Method ◽  
Long Term Storage ◽  
Term Storage

Highly efficient long-term storage of carrier-bound anammox biomass

2016 ◽  
Vol 74 (8) ◽  
pp. 1911-1918
Author(s):  
Steffen Engelbrecht ◽  
Mbengamina Terence Fondengcap ◽  
Kristina Rathsack ◽  
Marion Martienssen
Keyword(s):  
Specific Activity ◽  
Anammox Bacteria ◽  
Simple Method ◽  
Long Term Storage ◽  
Generation Times ◽  
Anammox Activity ◽  
Potential Alternative ◽  
Redox Buffer ◽  
Term Storage

The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 °C, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.

scholarly journals Effect of long-term storage on induced photon emission of boar spermatozoa

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 61-65 ◽  
Author(s):  
P. Gogol ◽  
B. Szczęśniak-Fabiańczyk
Keyword(s):  
Oxidative Damage ◽  
Sperm Motility ◽  
Photon Emission ◽  
Stress Factors ◽  
Simple Method ◽  
Long Term Storage ◽  
Boar Spermatozoa ◽  
Term Storage

Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was investigated using a luminometer during a 12-day storage at 15°C in Biosolwens extender. It was shown that the day of storage had a significant effect on some photon emission parameters. During storage, a significant increase in the Integral, Peak max. and Slope max. parameters and a decline in the T.-half (fall) parameter were observed. A significant correlation was observed between photon emission parameters and sperm motility. In conclusion, the results of the present study indicate that the measurement of induced photon emission can be an alternative, sensitive and relatively simple method for assessing the effect of preservation on oxidative damage to boar spermatozoa.  

Long-term storage of bradyrhizobia in sterile water

1993 ◽  
Vol 39 (10) ◽  
pp. 998-1001 ◽  
Author(s):  
Stephen C. Wagner ◽  
Horace D. Skipper
Keyword(s):  
Antibiotic Resistance ◽  
Bradyrhizobium Japonicum ◽  
Sterile Water ◽  
Simple Method ◽  
Antibiotic Resistant ◽  
Long Term Storage ◽  
Yeast Extract Mannitol ◽  
Resistant Strains ◽  
Term Storage

Preservation of strains of bradyrhizobia is an important aspect of legume research and development. Storage of strains of bradyrhizobia in sterile water is an inexpensive and simple method of preservation. This study was conducted to determine if bradyrhizobia strains remained viable after being stored in water for 66 months. Twenty-three bradyrhizobia strains were viable and pure after 66 months of storage in water. Nine Bradyrhizobium japonicum strains that were resistant to antibiotics remained viable and pure after 60 months of storage in water. However, three of the nine antibiotic-resistant strains lost antibiotic resistance after storage as assessed by failure to grow on yeast extract mannitol agar plates containing antibiotics. Maintenance in water should be limited to 48 months, at which time bradyrhizobia strains can be revived in media containing appropriate antibiotics and resuspended in water. These results demonstrated that bradyrhizobia strains can remain viable for at least 66 months of storage in water, but these bacteria may lose traits such as antibiotic resistance if stored too long in sterile water.Key words: bradyrhizobia, storage, water.

A simple method for long-term storage of Acanthamoeba species

2008 ◽  
Vol 104 (4) ◽  
pp. 935-937 ◽  
Author(s):  
Diana Axelsson-Olsson ◽  
Jenny Olofsson ◽  
Patrik Ellström ◽  
Jonas Waldenström ◽  
Björn Olsen
Keyword(s):  
Simple Method ◽  
Long Term Storage ◽  
Acanthamoeba Species ◽  
Term Storage

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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