Survival of wood-inhabiting fungi stored for 10 years in water and under oil

1992 ◽  
Vol 38 (8) ◽  
pp. 861-864 ◽  
Author(s):  
Gary C. Johnson ◽  
Andrew K. Martin

Subcultures of 1064 isolates of 393 species of wood-inhabiting fungi were stored both under sterile mineral oil and in sterile water for 10 years. A high proportion (98%) of the Basidiomycotina isolates survived storage under oil, whereas only 26% survived in sterile water. Survival of most isolates of Ascomycotina (91% under oil, 72% in water) and Deuteromycotina (96% under oil, 92% in water) was somewhat less in water than under oil. Key words: fungal storage, long-term storage, oil storage, water storage, Basidiomycotina.

1971 ◽  
Vol 36 (3) ◽  
pp. 322-335 ◽  
Author(s):  
Dennis E. Puleston

AbstractExperimental techniques have provided an exciting breakthrough for the functional analysis of Maya chultuns. While deep cistern-like chultuns, common at certain sites in the northern lowlands, have been shown to be functional for water storage, smaller lateral-chambered chultuns characteristic of certain parts of the southern lowlands probably had a very different function. Excavation and examination of the latter features, in light of a whole range of possibilities, suggest that they were constructed to be used for food storage. Experimental studies, however, reveal them to be unsuitable for the storage of most traditional foods, including maize. At least one local food crop, the seed of the ramon (Brosimum alicastrum, Moraceae), appears to be ideally suited for long-term storage under these conditions. Chambers constructed beneath platforms in the northern lowlands may have been used for the storage of maize. A need for more experimental work is indicated.


2012 ◽  
Vol 11 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Venessa van der Merwe ◽  
Stacey Duvenage ◽  
Lise Korsten

Rain-, ground- and municipal potable water were stored in low density polyethylene storage tanks for a period of 90 days to determine the effects of long-term storage on the deterioration in the microbial quality of the water. Total viable bacteria present in the stored water and the resultant biofilms were enumerated using heterotrophic plate counts. Polymerase chain reaction (PCR) and Colilert-18® tests were performed to determine if the faecal indicator bacteria Escherichia coli was present in the water and in the biofilm samples collected throughout the study. The municipal potable water at the start of the study was the only water source that conformed to the South African Water Quality Guidelines for Domestic Use. After 15 days of storage, this water source had deteriorated microbiologically to levels considered unfit for human consumption. E. coli was detected in the ground- and potable water and ground- and potable biofilms periodically, whereas it was detected in the rainwater and associated biofilms at every sampling point. Imperfections in the UV resistant inner lining of the tanks were shown to be ecological niches for microbial colonisation and biofilm development. The results from the current study confirmed that long-term storage can influence water quality and increase the number of microbial cells associated with biofilms on the interior surfaces of water storage tanks.


1979 ◽  
Vol 101 (2) ◽  
pp. 82-86
Author(s):  
M. A. Mahtab ◽  
D. W. Lamb ◽  
L. L. Van Sambeek ◽  
J. D. Gill

This paper, the first in a series of two, presents the results of a geotechnical evaluation of the Weeks Island dome salt mine. The purpose of the evaluation was to confirm the suitability of the underground facility for the long-term storage of crude oil under the Federal Energy Administration’s Strategic Petroleum Reserve Program. The Weeks Island mine, currently operated by the Morton Salt Company, is located in a salt dome on the Gulf Coast south of New Iberia, Louisiana. The mine has two levels of workings, at depths of approximately 185 and 245 m (600 and 800 ft) with development by the room-and-pillar mining technique. Geologic features of interest within the mine include zones of vertical banding and folding, shear zones, and blowouts. Field testing indicated that the salt mass is, for all intents and purposes, impermeable. Brine and oil leaks and gas seeps were examined and thought to be localized phenomena that did not affect mine stability and should not affect crude oil containment. The 23-m- (75-ft-) high pillars are generally intact with minor to severe spalling, generally in older areas of the mine. The mine roof exhibits no signs of distress or failure. Based on the overall results of the geotechnical and rock mechanics evaluations, the existing salt mine facility at Weeks Island was certified as suitable for the long-term storage of crude oil.


1989 ◽  
Vol 35 (11) ◽  
pp. 1055-1060 ◽  
Author(s):  
Dana L. Richter ◽  
Johann N. Bruhn

Vegetatively colonized agar cores of 135 basidiomycete isolates, representing 83 species in 38 genera, were stored at 5 °C in tubes of sterile distilled water. Viability, based on growth at room temperature, was determined after storage periods ranging from 3 to 48 months. Thirty-seven isolates of saprotrophic fungi represented 32 species of 22 genera; 98 isolates of mycorrhizal fungi represented 51 species of 16 genera. Most isolates of saprotrophic species were viable, even after the most extended periods of storage. Many isolates of mycorrhizal species demonstrated lower viability, especially over longer periods of storage. Among our isolates of mycorrhizal fungi, Laccaria bicolor (seven isolates) and Laccaria laccata (nine isolates) appeared well suited to this method of long-term storage, whereas Scleroderma species (22 isolates) did not.Key words: culture maintenance, culture viability, fungal preservation, long-term storage, vegetative cultures.


1986 ◽  
Vol 23 (4) ◽  
pp. 602-605 ◽  
Author(s):  
P. La Rochelle ◽  
S. Leroueil ◽  
F. Tavenas

This note describes a technique for sealing and storing clay samples so as to prevent aging effects during long periods of storage. It also gives the results of a study carried out on samples stored from 3 to 8 years, which prove the efficiency of that technique; the liquid limits, the liquidity indices, and the pH values remained constant during these long periods of storage. Key words: sensitive clay, storing, sealing, aging.


1986 ◽  
Vol 5 (5) ◽  
pp. 372-376 ◽  
Author(s):  
J. M. Augereau ◽  
D. Courtois ◽  
V. Petiard

1993 ◽  
Vol 39 (10) ◽  
pp. 998-1001 ◽  
Author(s):  
Stephen C. Wagner ◽  
Horace D. Skipper

Preservation of strains of bradyrhizobia is an important aspect of legume research and development. Storage of strains of bradyrhizobia in sterile water is an inexpensive and simple method of preservation. This study was conducted to determine if bradyrhizobia strains remained viable after being stored in water for 66 months. Twenty-three bradyrhizobia strains were viable and pure after 66 months of storage in water. Nine Bradyrhizobium japonicum strains that were resistant to antibiotics remained viable and pure after 60 months of storage in water. However, three of the nine antibiotic-resistant strains lost antibiotic resistance after storage as assessed by failure to grow on yeast extract mannitol agar plates containing antibiotics. Maintenance in water should be limited to 48 months, at which time bradyrhizobia strains can be revived in media containing appropriate antibiotics and resuspended in water. These results demonstrated that bradyrhizobia strains can remain viable for at least 66 months of storage in water, but these bacteria may lose traits such as antibiotic resistance if stored too long in sterile water.Key words: bradyrhizobia, storage, water.


2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.


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