scholarly journals Effect of long-term storage on induced photon emission of boar spermatozoa

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 61-65 ◽  
Author(s):  
P. Gogol ◽  
B. Szczęśniak-Fabiańczyk
Keyword(s):  
Oxidative Damage ◽  
Sperm Motility ◽  
Photon Emission ◽  
Stress Factors ◽  
Simple Method ◽  
Long Term Storage ◽  
Boar Spermatozoa ◽  
Term Storage

Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was investigated using a luminometer during a 12-day storage at 15°C in Biosolwens extender. It was shown that the day of storage had a significant effect on some photon emission parameters. During storage, a significant increase in the Integral, Peak max. and Slope max. parameters and a decline in the T.-half (fall) parameter were observed. A significant correlation was observed between photon emission parameters and sperm motility. In conclusion, the results of the present study indicate that the measurement of induced photon emission can be an alternative, sensitive and relatively simple method for assessing the effect of preservation on oxidative damage to boar spermatozoa.  

scholarly journals Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

2018 ◽  
Vol 64 (4) ◽  
pp. 351-360 ◽  
Author(s):  
Manuel ÁLVAREZ-RODRIGUEZ ◽  
Alejandro VICENTE-CARRILLO ◽  
Heriberto RODRIGUEZ-MARTINEZ
Keyword(s):  
Long Term Storage ◽  
Boar Spermatozoa ◽  
Term Storage

scholarly journals Cryopreservation of boar semen

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 115-123
Author(s):  
Marija Jovičić ◽  
Eva Chmelíková ◽  
Markéta Sedmíková
Keyword(s):  
Animal Species ◽  
Semen Quality ◽  
Egg Yolk ◽  
High Rate ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

2015 ◽  
Vol 31 ◽  
pp. 104-107 ◽  
Author(s):  
A. Fabbrocini ◽  
R. D'Adamo ◽  
S. Pelosi ◽  
L. F. J. Oliveira ◽  
F. Del Prete ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sparus Aurata ◽  
Sea Bream ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Evaluarea potenţialului de păstrare a seminţelor mostrelor din colecţia de Triticum durum L.

2021 ◽  
Author(s):  
Lolita Melian ◽  
◽  
Liudmila Corlateanu ◽  
Victoria Mihailă ◽  
Doina Cutitaru ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Durum ◽  
Accelerated Aging ◽  
Gene Bank ◽  
Stress Factors ◽  
Long Term Storage ◽  
Dry Biomass ◽  
Seedling Roots ◽  
Term Storage

The test of accelerated aging of seeds (AAS) was applied on 7 genotypes of durum wheat, and the morphophysiological parameters of seeds and seedlings, such as dynamics of seed germination, length of seedling roots, fresh and dry biomass of seedlings, were investigated. The genotypic features of collection samples of durum wheat were identified after exposure to stress factors (high temperature and humidity). Such a grouping of genotypes according to their potential ability to preserve the viability of seeds is an important complex characteristic of collection samples when they are placed for long-term storage in a plant gene bank.

scholarly journals ANALYSIS OF CRYOСONSERVED SPERM OF SIRES OF THE LEBEDINIAN BREED AND ORIGINAL BROWN CATTLE OF GERMANY

2019 ◽  
Vol 58 ◽  
pp. 95-101
Author(s):  
V. I. Ladyka ◽  
Y. I. Sklyarenko ◽  
Y. M. Pavlenko ◽  
O. V. Sherbak
Keyword(s):  
Sperm Motility ◽  
Sperm Production ◽  
Long Term Storage ◽  
Brown Cattle ◽  
Cryopreserved Sperm ◽  
Analysis System ◽  
Quantitative Indicators ◽  
Term Storage

In recent years, in Ukraine, as well as around the world, some local breeds have been on the verge of extinction, resulting in irretrievable loss of valuable genes, reduction of genetic diversity. In order to avoid these problems, it is necessary to work on the conservation of animal genetic resources. The creation of banks for long-term storage of biological material is one of the methods of preserving the gene pool of local, small and indigenous breeds of animals. Sperm quality is one of the main factors that determines the success of insemination of heifers and cows. Comprehensive analysis of sperm includes a large number of qualitative and quantitative indicators, the accuracy of which is influenced by a number of objective and subjective factors. Currently, microscopic analysis of sperm motility and survival is used in production conditions. Such approaches are easy to implement, but their results can be subjective. Research using a computer-based sperm fertility analysis system is becoming a priority today. With the help of CASA-Sperm Vision it is possible to carry out a morphological analysis already during the study of sperm motility. The purpose of our research was to analyze the quality of deep-frozen sperm of sires of Lebedinian breed and original brown cattle of Germany in order to attract such genetic material in the conservation of local Lebedinian breed cattle. Materials and methods. In the study used cryopreserved sperm doses of five sires of Lebedinian breed and three sires of the original brown cattle of Germany. Researches of qualitative, quantitative and dynamic characteristics of sperm of bulls were carried out at the laboratory of biotechnology of reproduction of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS and production laboratory "Ukrainian genetic company" using computer analysis of sperm motility Sperm Analysis System Version 12 IVOS. It was determined that the average dose of cryopreserved sperm of bulls of the Lebedinian breed was 0.25 ml, and of the sires of the original brown cattle of Germany -0.21 ml. It was found that the sires of the original brown cattle of Germany had the best indicators of total sperm motility by 21.7% compared to the sires of the Lebedinian breed (55.7%). They also noted a greater number of sperm with straight-forward movement of 16.5%. (40.2% of the Lebedinian breed sires). The average concentration of sperm in one milliliter was 10.5 times higher in animals of the Lebedinian breed (compared to the sires of the original brown cattle of Germany (331 million/ml). Comparing the indicators of the average speed of the sperm on the average trajectory (VAP), it should be noted that the minimum value was 85.3 microns/sec, the maximum value for this indicator – 141.7 microns/sec. The results of the VSL study showed that the average value of this indicator of sperm in the studied sperm doses of sires of the Lebedinian breed was 102 microns/sec, of the original brown cattle of Germany – 75. Obtained opposite to the direction coefficients of the correlation depending on the origin, between total sperm motility and average sperm speed for the average trajectory, the average speed of the sperm on the real trajectory, the average deviation of the sperm, the degree of straightness of the directed movement of sperm. Summary. 1. The complex of researches provided an objective analysis of qualitative and quantitative indicators of cryopreserved sperm production of the bulls of original brown cattle of Germany and the Lebedinian breed. It was found that the quality of the studied sperm doses met the requirements of "Instructions for Artificial Insemination of Cows and Heifers" (Order of the Ministry of Agrarian Policy of Ukraine dated August 1, 2001 № 230). 2. The comparative analysis of sperm production of the original brown cattle of Germany and the Lebedinian breed for its long-term storage was carried out. It is established that the sperm of sires of the original brown cattle of Germany exhibit higher dynamic characteristics of movement, while the sperm of sires of the Lebedinian breed were inferior to them in these indicators. 3. The possibility of insemination of females with cryopreserved sperm of the studied bulls whose sperm production has been stored for more than 30 years has been proved.

Inter- and intra-breed comparative study of sperm motility and viability in Iberian and Duroc boar semen during long-term storage in MR-A and XCell extenders

2013 ◽  
Vol 139 (1-4) ◽  
pp. 109-114 ◽  
Author(s):  
D. Martín-Hidalgo ◽  
F.J. Barón ◽  
A. Robina ◽  
M.J. Bragado ◽  
A. Hurtado de Llera ◽  
...  
Keyword(s):  
Comparative Study ◽  
Sperm Motility ◽  
Long Term Storage ◽  
Boar Semen ◽  
Term Storage

Effect of long term storage in LN2 on bacterial load and preservability of semen in Murrah bulls

2015 ◽  
Author(s):  
G. S. Meena ◽  
V. S. Raina ◽  
M. Bhakat ◽  
T. K. Mohanty ◽  
A. K. Gupta ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Incubation Period ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Bacterial Load ◽  
Storage Period ◽  
Least Square ◽  
Long Term Storage ◽  
Term Storage

Successful preservation of semen for longer period can be achieved by maintaining the viability of spermatozoa from its collection till its use for insemination. Therefore, we perceived the idea to determine the effect of long term storage of cryopreserved buffalo semen on sperm quality, bacterial load and fertility. Twenty years semen samples (1981-2000) from twenty bulls and the data on conception rates of these bulls were collected from record room at ABRC, ICAR- NDRI, Karnal. The data was analyzed using least square analysis. The differences in individual motility percent in the semen between estimated initially at the time of freezing and estimates after storage (at the time of evaluation) were 6.49, 14.09, 13.13 and 6.02 percent, respectively. Even after long term storage (up to 20 years) there were little changes in the sperm motility percent. Changes in non-eosinophilic sperm count, sperm abnormalities, HOST and acrosome status, were less up to 20 years storage of semen in liquid nitrogen and the differences were non-significant between different years of storage semen in liquid nitrogen. In similar fashion the microbial load in semen was decreased with the increased storage period of semen in liquid nitrogen, but the differences were non significant. The differences in sperm motility percent between 370C and room temperature were not significant, but the temperature and incubation period significantly (P<0.01) influenced motility percent estimates. Whereas, temperature-incubation period interaction was not found to be significant. The conception rate and sperm motility was highly and positively correlated (r=0.67 and 0.55) with sperm oocyte attachment. It can be concluded that there was little change occurred in semen quality even after twenty years of storage in liquid nitrogen without affecting fertility of semen.

scholarly journals Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

2020 ◽  
Vol 21 (18) ◽  
pp. 6474
Author(s):  
Lisa Höfner ◽  
Anne-Marie Luther ◽  
Alessandra Palladini ◽  
Thomas Fröhlich ◽  
Dagmar Waberski
Keyword(s):  
Mass Spectrometry ◽  
Seminal Plasma ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Protein Markers ◽  
Long Term Storage ◽  
Lipidome Analysis ◽  
Boar Spermatozoa ◽  
Term Storage

Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.

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