Induction of G-bands in the chromosomes of Melanoplus sanguinipes (Orthoptera, Acrididae)

1984 ◽  
Vol 26 (3) ◽  
pp. 354-359 ◽  
Author(s):  
T. S. Zhan ◽  
S. Pathak ◽  
J. C. Liang
Keyword(s):  
Banding Pattern ◽  
Actinomycin D ◽  
Centromeric Region ◽  
Constitutive Heterochromatin ◽  
Entire Length ◽  
Giemsa Staining ◽  
Human Chromosomes ◽  
Melanoplus Sanguinipes ◽  
Longitudinal Differentiation ◽  
Chromosome Ii

Pretreatment of embryos of a nondiapause strain of the migratory grasshopper (Melanoplus sanguinipes) with actinomycin D, followed by posttreatment of chromosome preparations obtained from them with NaOH and Sörenson's buffer, induced longitudinal differentiation of each chromosome after Giemsa staining. We consider such crossbands to be G-bands because of the following: (i) they were present along the entire length of each chromosome; (ii) the centromeric region of each chromosome was unstained; (iii) each pair of chromosomes showed its own distinctive banding pattern; (iv) the banding pattern was consistent from cell to cell for the same pair of chromosomes; and (v) a similar procedure is known to induce G-bands in human chromosomes. C-bands induced by the Ba(OH)2 technique showed constitutive heterochromatin to be confined to the centromeric region of each chromosome.Key words: Melanoplus, G-bands.

Late replicating bands of human chromosomes demonstrated by fluorochrome and Giemsa staining

1975 ◽  
Vol 29 (1) ◽  
pp. 41-59 ◽  
Author(s):  
K. -H. Grzeschik ◽  
My. A. Kim ◽  
Renate Johannsmann
Keyword(s):  
Giemsa Staining ◽  
Human Chromosomes

Structural basis of banding pattern of human chromosomes

1973 ◽  
Vol 12 (2) ◽  
pp. 81-86 ◽  
Author(s):  
J. Cervenka ◽  
Hattie L. Thorn ◽  
R.J. Gorlin
Keyword(s):  
Banding Pattern ◽  
Structural Basis ◽  
Human Chromosomes

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes ◽  
Nick Translation ◽  
In Situ Nick Translation ◽  
Eco Ri

Different reactivity of Z-DNA antibodies with human chromosomes modified by actinomycin D and 5-bromodeoxyuridine

1987 ◽  
Vol 75 (2) ◽  
pp. 114-119 ◽  
Author(s):  
E. Viegas-Pequignot ◽  
B. Malfoy ◽  
L. Sabatier ◽  
B. Dutrillaux
Keyword(s):  
Actinomycin D ◽  
Human Chromosomes ◽  
Dna Antibodies ◽  
Z Dna

G and/or C-bands in plant chromosomes?

1984 ◽  
Vol 71 (1) ◽  
pp. 111-120
Author(s):  
I. Schubert ◽  
R. Rieger ◽  
P. Dobel
Keyword(s):  
Constitutive Heterochromatin ◽  
Giemsa Staining ◽  
Giemsa Banding ◽  
Base Pairs ◽  
Banding Patterns ◽  
C Banding ◽  
Plant Chromosomes ◽  
Nucleolus Organizing Region ◽  
Similarities And Differences ◽  
Simple Sequence

Similarities and differences become evident from comparisons of centromeric and non-centromeric banding patterns in plant and animal chromosomes. Similar to C and G-banding in animals (at least most of the reptiles, birds and mammals), centromeric and nucleolus-organizing region bands as well as interstitially and/or terminally located non-centromeric bands may occur in plants, depending on the kind and strength of pretreatment procedures. The last group of bands may sometimes be subdivided into broad regularly occurring ‘marker’ bands and thinner bands of more variable appearance. Non-centromeric bands in plants often correspond to blocks of constitutive heterochromatin that are rich in simple sequence DNA and sometimes show polymorphism; they thus resemble C-bands. However, most of these bands contain late-replicating DNA. Also they are sometimes rich A X T base-pairs, closely adjacent to each other and positionally identical to Feulgen+ and Q+ bands, thus being comparable to mammalian G-bands. Although banding that is reverse to the non-centromeric bands after Giemsa staining is still uncertain in plants, reverse banding patterns can be obtained with Feulgen or with pairs of A X T versus G X C-specific fluorochromes. It is therefore concluded that not all of the plant Giemsa banding patterns correspond to C-banding of mammalian chromosomes. Before the degree of homology between different Giemsa banding patterns in plants and G and/or C-bands in mammals is finally elucidated, the use of the neutral term ‘Giemsa band’, specified by position (e.g. centromeric, proximal, interstitial, terminal), is suggested to avoid confusion.

Analysis of high-resolution R-bands, obtained by heat-denaturation and Giemsa staining, on human prophase chromosomes

1985 ◽  
Vol 27 (1) ◽  
pp. 83-91 ◽  
Author(s):  
R. Drouin ◽  
C. L. Richer
Keyword(s):  
High Resolution ◽  
Banding Pattern ◽  
Giemsa Staining ◽  
Heat Denaturation ◽  
Banding Patterns ◽  
International Standard

RHG-bands (heat-denatured Giemsa R-bands) of human prophase chromosomes were analyzed at high resolution, and the banding patterns at prophase and metaphase are presented. The bands were compared with those of the International Standard Cytogenetic Nomenclature idiograms and of the G-band idiograms proposed by J. J. Yunis. The number, size, and position of the RHG-bands correspond rather well with their equivalent G-negative bands, but some differences were noted in the zones of preferential stretching, the juxtacentromeric regions, and the telomeres. Variations in the centromere index and the banding pattern in heterochromatin were also discussed.Key words: human prophase chromosomes, RHG-bands, high-resolution chromosomes.

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