Analysis of high-resolution R-bands, obtained by heat-denaturation and Giemsa staining, on human prophase chromosomes

1985 ◽  
Vol 27 (1) ◽  
pp. 83-91 ◽  
Author(s):  
R. Drouin ◽  
C. L. Richer
Keyword(s):  
High Resolution ◽  
Banding Pattern ◽  
Giemsa Staining ◽  
Heat Denaturation ◽  
Banding Patterns ◽  
International Standard

RHG-bands (heat-denatured Giemsa R-bands) of human prophase chromosomes were analyzed at high resolution, and the banding patterns at prophase and metaphase are presented. The bands were compared with those of the International Standard Cytogenetic Nomenclature idiograms and of the G-band idiograms proposed by J. J. Yunis. The number, size, and position of the RHG-bands correspond rather well with their equivalent G-negative bands, but some differences were noted in the zones of preferential stretching, the juxtacentromeric regions, and the telomeres. Variations in the centromere index and the banding pattern in heterochromatin were also discussed.Key words: human prophase chromosomes, RHG-bands, high-resolution chromosomes.

High-resolution idiogram of Giemsa R-banded human prophase chromosomes

1983 ◽  
Vol 25 (6) ◽  
pp. 642-650 ◽  
Author(s):  
C. L. Richer ◽  
R. Drouin ◽  
M. Murer-Orlando ◽  
P. Jean
Keyword(s):  
Comparative Analysis ◽  
High Resolution ◽  
Relative Position ◽  
Chromosomal Rearrangements ◽  
Staining Intensity ◽  
Chromosome Condensation ◽  
Giemsa Staining ◽  
Heat Denaturation ◽  
Schematic Representation ◽  
International Standard

The schematic representation of RHG-banded chromosomes (R-banding was produced by heat denaturation followed by Giemsa staining (RHG)) in the 850-band range per haploid set, was prepared showing the relative position, the specific size, and the characteristic staining intensity for each band. To this idiogram was adapted the new International Standard Cytogenetic Nomenclature. Our aim was to produce a realisitic idiogram which could help in the preparation of R-banded prophase karyotypes and in the localization of chromosomal rearrangements. A comparative analysis of bands at prophase and metaphase revealed certain aspects of the dynamics involved in chromosome condensation and in R-band organization. The effect of chromosome elongation on the appearance of R-bands within heterochromatic regions has also been discussed.

Trisomy 21 for the region 21q223: Identification by high-resolution R-banding patterns

1981 ◽  
Vol 56 (3) ◽  
pp. 409-411 ◽  
Author(s):  
J. F. Mattei ◽  
M. G. Mattei ◽  
M. A. Baeteman ◽  
F. Giraud
Keyword(s):  
High Resolution ◽  
Trisomy 21 ◽  
Banding Patterns

scholarly journals Involement of an acrosinlike proteinase in the sulfhydryl-induced degradation of rabbit sperm nuclear protamine

1980 ◽  
Vol 85 (1) ◽  
pp. 116-121 ◽  
Author(s):  
BR Zirkin ◽  
TSK Chang ◽  
J Heaps
Keyword(s):  
Proteolytic Activity ◽  
Banding Pattern ◽  
Basic Protein ◽  
Polyacrylamide Gels ◽  
Banding Patterns ◽  
Pattern Characteristic ◽  
Sperm Nuclei ◽  
Triton X ◽  
Esterase Inhibitor

Previous studies demonstrated that proteolytic activity is associated with isolated rabbit sperm nuclei and is responsible for the degradation of nuclear protamine that occurs during thiol-induced in vitro decondensation of the nuclei (Zirkin and Chang, 1977; Chang and Zirkin, 1978). In this study, we present the results of experiments designed to characterize this proteolytic activity. Basic protein isolated from rabbit sperm nuclei incubated with 5 mM dithiothreitol (DTT) and 1 percent Triton X-100 for increasing periods of time exhibited progressively faster migrating bands on acid-urea polyacrylamide gels, reflection the progressive degradation of protamine. Ultimately, a specific and characteristic peptide banding pattern resulted. When sperm nuclei were treated with the esterase inhibitor nitrophenyl-p-guanidino benzoate (NPGB) to inhibit the nuclear-associated proteolytic activity and then incubated with one of several exogenous proteinases in addition to DTT and Triton X-100, characteristic peptide banding patterns were seen for each exogenous proteinase employed. For trypsin, chymotrypsin, pronase, and papain, the peptide banding patterns differed from one another and from the pattern characteristic of protamine degradation by the nuclear-associated proteinase. By contrast, when rabbit acrosin served as the exogenous proteinase, the peptide banding pattern seen was identical to the pattern characteristic of the nuclear-associated proteinase. These results demonstrate directly that the proteinase associated with rabbit sperm nuclei and involved in sperm nuclear decondensation in vitro is acrosinlike.

A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 215-219 ◽  
Author(s):  
A. Zacharopoulou ◽  
K. Bourtzis ◽  
Ph. Kerremans
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Banding Pattern ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes ◽  
Biological Significance ◽  
Fruit Fly ◽  
Banding Patterns ◽  
Homologous Chromosomes ◽  
Different Tissues

The banding patterns of polytene chromosomes in different tissues of the Mediterranean fruit fly, Ceratitis capitata, vary to such an extent that homologous chromosomes cannot be recognised. However, analyses of autosomal breakpoints in several translocation strains allowed chromosomes from the two tissues to be aligned despite their difference in banding pattern. These results were discussed, considering the different hypotheses of the origin and biological significance of polytene chromosome bands.Key words: polytene chromosomes, salivary gland chromosomes, orbital bristle trichogen cell chromosomes, Ceratitis capitata.

G-banding patterns of high-resolution human chromosomes 6?22, X, and Y

1979 ◽  
Vol 49 (3) ◽  
pp. 291-306 ◽  
Author(s):  
J. J. Yunis ◽  
D. W. Ball ◽  
J. R. Sawyer
Keyword(s):  
High Resolution ◽  
Human Chromosomes ◽  
Banding Patterns

High resolution G-banding patterns of Syrian hamster chromosomes

1982 ◽  
Vol 33 (4) ◽  
pp. 295-302 ◽  
Author(s):  
S. Li ◽  
S. Pathak ◽  
T.C. Hsu
Keyword(s):  
High Resolution ◽  
Syrian Hamster ◽  
Banding Patterns

scholarly journals Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018 ◽  
Vol 19 (5) ◽  
pp. 1683-1689 ◽  
Author(s):  
NUR RAHMAH HIDAYATI ◽  
SURANTO SURANTO ◽  
SAJIDAN SAJIDAN
Keyword(s):  
Banding Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Retardation Factor ◽  
Cucurbita Moschata ◽  
Esterase Isozyme ◽  
Banding Patterns ◽  
Different Altitudes

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

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