Structural basis of banding pattern of human chromosomes

1973 ◽  
Vol 12 (2) ◽  
pp. 81-86 ◽  
Author(s):  
J. Cervenka ◽  
Hattie L. Thorn ◽  
R.J. Gorlin
Keyword(s):  
Banding Pattern ◽  
Structural Basis ◽  
Human Chromosomes

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes ◽  
Nick Translation ◽  
In Situ Nick Translation ◽  
Eco Ri

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

Differential Acridine Orange Staining of Human Chromosomes

1972 ◽  
Vol 21 (4) ◽  
pp. 319-326
Author(s):  
G.L. Castoldi ◽  
G.D. Grusovin ◽  
G.L. Scapoli ◽  
R. Spanedda
Keyword(s):  
Acridine Orange ◽  
Banding Pattern ◽  
Human Chromosomes ◽  
Salt Solutions ◽  
Single Stranded Dna ◽  
Acridine Orange Staining ◽  
Double Stranded Dna ◽  
Previously Treated

SummaryThe acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

Banding Pattern of Human Chromosomes

1971 ◽  
Vol 233 (37) ◽  
pp. 93-94 ◽  
Author(s):  
W. SCHNEDL
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes

Induction of G-bands in the chromosomes of Melanoplus sanguinipes (Orthoptera, Acrididae)

1984 ◽  
Vol 26 (3) ◽  
pp. 354-359 ◽  
Author(s):  
T. S. Zhan ◽  
S. Pathak ◽  
J. C. Liang
Keyword(s):  
Banding Pattern ◽  
Actinomycin D ◽  
Centromeric Region ◽  
Constitutive Heterochromatin ◽  
Entire Length ◽  
Giemsa Staining ◽  
Human Chromosomes ◽  
Melanoplus Sanguinipes ◽  
Longitudinal Differentiation ◽  
Chromosome Ii

Pretreatment of embryos of a nondiapause strain of the migratory grasshopper (Melanoplus sanguinipes) with actinomycin D, followed by posttreatment of chromosome preparations obtained from them with NaOH and Sörenson's buffer, induced longitudinal differentiation of each chromosome after Giemsa staining. We consider such crossbands to be G-bands because of the following: (i) they were present along the entire length of each chromosome; (ii) the centromeric region of each chromosome was unstained; (iii) each pair of chromosomes showed its own distinctive banding pattern; (iv) the banding pattern was consistent from cell to cell for the same pair of chromosomes; and (v) a similar procedure is known to induce G-bands in human chromosomes. C-bands induced by the Ba(OH)2 technique showed constitutive heterochromatin to be confined to the centromeric region of each chromosome.Key words: Melanoplus, G-bands.

Organization of tight junctions in the choroid plexus epithelium

1978 ◽  
Vol 36 (2) ◽  
pp. 336-337
Author(s):  
B. Van Deurs ◽  
J. K. Koehler
Keyword(s):  
Choroid Plexus ◽  
Present Report ◽  
Freeze Fracture ◽  
Barrier Properties ◽  
Cell Junctions ◽  
Structural Basis ◽  
Apical Surface ◽  
Choroid Plexus Epithelium ◽  
Solid Nitrogen ◽  
Special Composition

The choroid plexus epithelium constitutes a blood-cerebrospinal fluid (CSF) barrier, and is involved in regulation of the special composition of the CSF. The epithelium is provided with an ouabain-sensitive Na/K-pump located at the apical surface, actively pumping ions into the CSF. The choroid plexus epithelium has been described as “leaky” with a low transepithelial resistance, and a passive transepithelial flux following a paracellular route (intercellular spaces and cell junctions) also takes place. The present report describes the structural basis for these “barrier” properties of the choroid plexus epithelium as revealed by freeze fracture.Choroid plexus from the lateral, third and fourth ventricles of rats were used. The tissue was fixed in glutaraldehyde and stored in 30% glycerol. Freezing was performed either in liquid nitrogen-cooled Freon 22, or directly in a mixture of liquid and solid nitrogen prepared in a special vacuum chamber. The latter method was always used, and considered necessary, when preparations of complementary (double) replicas were made.

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