The metaphase banding pattern produced in the chromosomes of Culiseta longiareolata (Diptera: Culicidae) by AluI restriction endonuclease or actinomycin-D treatment followed by acridine-orange staining

Genetica ◽  
1983 ◽  
Vol 62 (1) ◽  
pp. 47-50 ◽  
Author(s):  
R. Mezzanotte ◽  
L. Ferrucci
Keyword(s):  
Acridine Orange ◽  
Restriction Endonuclease ◽  
Banding Pattern ◽  
Actinomycin D ◽  
Acridine Orange Staining ◽  
Culiseta Longiareolata

Differential Acridine Orange Staining of Human Chromosomes

1972 ◽  
Vol 21 (4) ◽  
pp. 319-326
Author(s):  
G.L. Castoldi ◽  
G.D. Grusovin ◽  
G.L. Scapoli ◽  
R. Spanedda
Keyword(s):  
Acridine Orange ◽  
Banding Pattern ◽  
Human Chromosomes ◽  
Salt Solutions ◽  
Single Stranded Dna ◽  
Acridine Orange Staining ◽  
Double Stranded Dna ◽  
Previously Treated

SummaryThe acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

scholarly journals EVIDENCE FOR VARIATION IN THE QUANTITY OF DNA AMONG PLASTIDS OF ACETABULARIA

1970 ◽  
Vol 44 (2) ◽  
pp. 361-375 ◽  
Author(s):  
Christopher L. F. Woodcock ◽  
Lawrence Bogorad
Keyword(s):  
Electron Microscopy ◽  
Acridine Orange ◽  
Dna Content ◽  
Actinomycin D ◽  
Acridine Orange Staining ◽  
Tissue Sections ◽  
Unicellular Algae ◽  
Acetabularia Mediterranea

The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-3H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily detected in 20–35% of plastids, but no DNA was observed in the remaining 65–80%. The results further showed that in those plastids with detectable DNA the amount of DNA present was variable. The sensitivity and reliability of the localization methods are discussed, and the possible implications of these findings are considered.

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 535-540 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Acridine Orange ◽  
Particle Number ◽  
Amoeba Proteus ◽  
Acridine Orange Staining ◽  
Particle Population ◽  
Cell Age ◽  
Cytoplasmic Dna ◽  
Number Of Particles

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.

The fluorescent staining of a mycobacteriophage (C2) nucleic acid

1971 ◽  
Vol 17 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Jose Menezes
Keyword(s):  
Nucleic Acid ◽  
Acridine Orange ◽  
Staining Technique ◽  
Fluorescent Staining ◽  
Green Fluorescence ◽  
Acridine Orange Staining ◽  
Fluorescence Characteristic ◽  
Phage Dna ◽  
Acid Dnase

By using the acridine orange staining technique a green fluorescence, characteristic of double-stranded nucleic acid, can be observed with purified preparations of mycobacteriophage C2 and its extracted nucleic acid. DNAse-treated samples do not show this fluorescence, which leads to the conclusion that this fluorescence is associated with phage DNA. Examination of preparations of phage grown in the presence of acridine orange supported these results.

An improved acridine orange staining of DNA/RNA

2019 ◽  
Vol 121 (4) ◽  
pp. 450-454 ◽  
Author(s):  
Danilo M. Damas-Souza ◽  
Rony Nunes ◽  
Hernandes F. Carvalho
Keyword(s):  
Acridine Orange ◽  
Acridine Orange Staining
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