Late replicating bands of human chromosomes demonstrated by fluorochrome and Giemsa staining

1975 ◽  
Vol 29 (1) ◽  
pp. 41-59 ◽  
Author(s):  
K. -H. Grzeschik ◽  
My. A. Kim ◽  
Renate Johannsmann
Keyword(s):  
Giemsa Staining ◽  
Human Chromosomes

R-banding of human chromosomes by heat denaturation and Giemsa staining after amethopterin-synchronization

1983 ◽  
Vol 25 (3) ◽  
pp. 261-269 ◽  
Author(s):  
C. L. Richer ◽  
M. Murer-Orlando ◽  
R. Drouin
Keyword(s):  
Giemsa Staining ◽  
Heat Denaturation ◽  
Human Chromosomes ◽  
Metaphase Chromosomes ◽  
Late Prophase ◽  
Chromosome Anomalies ◽  
Cell Synchronization

Human late prophase to late metaphase chromosomes were prepared after amethopterin cell synchronization. R-banding was produced by heat denaturation followed by Giemsa staining (RHG). Haploid sets of prophase chromosomes contain approximately 850 bands. Sequences of chromosomes of different degrees of condensation are presented; their analysis provides helpful information to identify the elongated chromosomes and to follow band subdivision. The heat denaturation technique is free from most of the disadvantages encountered with R-banding by 5-bromodeoxyuridine incorporation. Giemsa stained R-bands produced by heat denaturation on prophase and prometaphase chromosomes are useful to analyse the numerous chromosome anomalies involving R-bands. In conjunction with G-banding, it is also important to compare adequately the positive and negative regions of each chromosome to define the anomalies with precision.

Induction of G-bands in the chromosomes of Melanoplus sanguinipes (Orthoptera, Acrididae)

1984 ◽  
Vol 26 (3) ◽  
pp. 354-359 ◽  
Author(s):  
T. S. Zhan ◽  
S. Pathak ◽  
J. C. Liang
Keyword(s):  
Banding Pattern ◽  
Actinomycin D ◽  
Centromeric Region ◽  
Constitutive Heterochromatin ◽  
Entire Length ◽  
Giemsa Staining ◽  
Human Chromosomes ◽  
Melanoplus Sanguinipes ◽  
Longitudinal Differentiation ◽  
Chromosome Ii

Pretreatment of embryos of a nondiapause strain of the migratory grasshopper (Melanoplus sanguinipes) with actinomycin D, followed by posttreatment of chromosome preparations obtained from them with NaOH and Sörenson's buffer, induced longitudinal differentiation of each chromosome after Giemsa staining. We consider such crossbands to be G-bands because of the following: (i) they were present along the entire length of each chromosome; (ii) the centromeric region of each chromosome was unstained; (iii) each pair of chromosomes showed its own distinctive banding pattern; (iv) the banding pattern was consistent from cell to cell for the same pair of chromosomes; and (v) a similar procedure is known to induce G-bands in human chromosomes. C-bands induced by the Ba(OH)2 technique showed constitutive heterochromatin to be confined to the centromeric region of each chromosome.Key words: Melanoplus, G-bands.

Automatic Karyotyping of Human Chromosomes Using Band Patterns

2012 ◽  
Vol 2 (12) ◽  
pp. 154-156 ◽  
Author(s):  
Priya Chaku ◽  
◽  
Pooja Shah
Keyword(s):  
Human Chromosomes

Linkage of genetic markers on human chromosomes 20 and 12 to NIDDM in Caucasian sib pairs with a history of diabetic nephropathy

Diabetes ◽  
1997 ◽  
Vol 46 (5) ◽  
pp. 882-886 ◽  
Author(s):  
D. W. Bowden ◽  
M. Sale ◽  
T. D. Howard ◽  
A. Qadri ◽  
B. J. Spray ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Genetic Markers ◽  
Human Chromosomes ◽  
History Of ◽  
Sib Pairs
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