Effects of pH, Temperature, and Ultraviolet Radiation on the Activity of an Optical Brightener as a Viral Enhancer for the Gypsy Moth (Lepidoptera: Lymantriidae) Baculovirus

1995 ◽  
Vol 88 (6) ◽  
pp. 1602-1606 ◽  
Author(s):  
Martin Shapiro ◽  
Robert Argauer
2012 ◽  
Vol 32 (6) ◽  
pp. 1796-1802
Author(s):  
王树娟 WANG Shujuan ◽  
段立清 DUAN Liqing ◽  
李海平 LI Haiping ◽  
马涛 MA Tao ◽  
Otvos. I.S Otvos. I.S ◽  
...  

2003 ◽  
Vol 135 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Martin Shapiro ◽  
Robert A. Argauer

AbstractThe stilbenes 4,4′-diaminostilbene-2,2′-disulfonic acid, 4-aminostilbene disulfonic acid, and dinitrostilbene-2,2′-disulfonic acid were tested as enhancers for the gypsy moth, Lymantria dispar (L.), nucleopolyhedrovirus (LdNPV). 4-Amino nitrostilbene disulfonic acid had no effect on the activity (LC50) of LdNPV, whereas both 4,4′-diaminostilbene-2,2′-disulfonic acid and 4-aminostilbene disulfonic acid were inhibitory. Diethylstilbestrol, a stilbene synthetic estrogen, and two synthetic estrogens (i.e., estradiol-17-acetate, estrone acetate) had no effects on viral activity. Two stilbene dyes (i.e., direct yellow 62, brilliant yellow 6) and a stilbene optical brightener (i.e., Tinopal LPW) significantly increased the activity of LdNPV. Activity was increased by approximately 230-fold by Tinopal LPW, 26-fold by direct yellow 62, and 36-fold by brilliant yellow 6. This study demonstrates that some stilbenes can act as enhancers, whereas others do not.


Author(s):  
William J. Baxter

In this form of electron microscopy, photoelectrons emitted from a metal by ultraviolet radiation are accelerated and imaged onto a fluorescent screen by conventional electron optics. image contrast is determined by spatial variations in the intensity of the photoemission. The dominant source of contrast is due to changes in the photoelectric work function, between surfaces of different crystalline orientation, or different chemical composition. Topographical variations produce a relatively weak contrast due to shadowing and edge effects.Since the photoelectrons originate from the surface layers (e.g. ∼5-10 nm for metals), photoelectron microscopy is surface sensitive. Thus to see the microstructure of a metal the thin layer (∼3 nm) of surface oxide must be removed, either by ion bombardment or by thermal decomposition in the vacuum of the microscope.


Author(s):  
Beverly E. Maleeff ◽  
Timothy K. Hart ◽  
Stephen J. Wood ◽  
Ronald Wetzel

Alzheimer's disease is characterized post-mortem in part by abnormal extracellular neuritic plaques found in brain tissue. There appears to be a correlation between the severity of Alzheimer's dementia in vivo and the number of plaques found in particular areas of the brain. These plaques are known to be the deposition sites of fibrils of the protein β-amyloid. It is thought that if the assembly of these plaques could be inhibited, the severity of the disease would be decreased. The peptide fragment Aβ, a precursor of the p-amyloid protein, has a 40 amino acid sequence, and has been shown to be toxic to neuronal cells in culture after an aging process of several days. This toxicity corresponds to the kinetics of in vitro amyloid fibril formation. In this study, we report the biochemical and ultrastructural effects of pH and the inhibitory agent hexadecyl-N-methylpiperidinium (HMP) bromide, one of a class of ionic micellar detergents known to be capable of solubilizing hydrophobic peptides, on the in vitro assembly of the peptide fragment Aβ.


2006 ◽  
Vol 175 (4S) ◽  
pp. 260-260
Author(s):  
Nicholas J. Rukin ◽  
Samuel J. Moon ◽  
Dhaval Bodiwala ◽  
Christopher J. Luscombe ◽  
Mark F. Saxby ◽  
...  

1992 ◽  
Vol 86 (1) ◽  
pp. 63-70 ◽  
Author(s):  
David Brauer ◽  
DeNea Conner ◽  
Shu-I Tu

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