Experimental studies of a machine for collecting the Colorado potato beetle

ional Academy of Sciences of Belarus, Minsk, the Republic of Belarus The substantiation of the design and technological scheme of the machine for the mechanical collection of the Colorado potato beetle in the production of environmentally friendly potato is carried out. The parameters and modes of rotors with flexible-elastic blades and regulators of the amplitude of their oscillations, providing high-quality shaking and collection of Colorado potato beetle individuals from the tops with minimal damage, have been experimentally substantiated. A description of the laboratory installation, an experimental sample of a combined unit is presented, and a methodology for conducting experiments is described. Experimental studies were carried out in 2004-2008 in the experimental field of the Educational institution "Grodno State Agrarian University" (UO "GGAU") and in the fields of the Agricultural production cooperative (SEC) "Zanemansky" of the Mostovsky dis-trict of the Grodno region. It was found that in order to reduce the energy intensity of the process, the interaction of the rotor with the tops in its apical part, where the maximum con-centration of Colorado potato beetle individuals takes place, is expedient. The minimum amount of beetle on the tops and the absence of visible damage to it are achieved when the diameter of the nylon threads of the blade is 1.2-1.5 mm, and the rational position of the regulator from the rotor axis is 0.14-0.18 m according to the energy intensity condition. Under the condition of minimal energy consumption of the process and without visible damage to the leaves, the circumferential speed range of the rotor should be 3-4 m/s. A regression equation of the second degree is obtained, which determines the relationship of the residual number of beetle individuals on the tops after the passage of the machine with the circumferential speed of the rotor Voc, the position of the regulator on the radius of the rotor Rr and the cross section of the blade Sbl. The optimal values were determined by solving the equation: Voc = 3.7 m/s, Rr = 0.16 m, Sbl = 1800 mm2 .

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious, widely distributed pest of potato and other crops. This pest is able to defoliate the host plant and cause severe yield loss. Moreover, the pest quickly becomes resistant to many chemical pesticides. Therefore, the development of novel biopesticides targeting this pest is urgently needed. The purpose of this study was to obtain new strains of the entomopathogenic fungus Beauveria bassiana and assess their efficacy against L. decemlineata adults under laboratory conditions. Twelve strains were isolated from cadavers of Colorado potato beetles collected in potato fields in the Czech Republic. Test beetles were treated by suspensions of conidia at the concentration of 1 × 107 spores per milliliter and their survival was recorded daily for three weeks. The results of the bioassays revealed that all new native strains were pathogenic to L. decemlineata adults and caused mortality up to 100% at the end of the trial period with an LT50 of about 7 days. These strains were more virulent than a reference strain GHA and some of them can be recommended for the development of a new mycoinsecticide against L. decemlineata. Our findings also highlight the importance of searching for perspective strains of entomopathogenic fungi among naturally infected hosts.

Low-Dose Insecticide Combinations for Colorado Potato Beetle Control

Colorado potato beetle (CPB) is the most notorious and problematic insect defoliator pest of potato. It is well known for its ability to develop resistance to chemical insecticides, so novel treatment methods must be developed. A possible solution is the application of newer groups of insecticides (biotechnical and biological insecticides) which are more selective and to which resistance has not yet been recorded. This work investigated the use of ecologically acceptable insecticide treatments: azadirachtin, spinosad and spinetoram, and their combinations against CPB larvae in laboratory and field conditions. Reduced dosing and combinational treatments were used to determine if effective protection against CPB could be achieved while also improving economic results. Our results from laboratory trials showed that combinations of low-dose azadirachtin (25%) and low-dose spinetoram (5%) are suitable for use in CPB integrated pest management. The results of the field trial showed that the use of 50% reduced dosage of spinosad and spinetoram resulted in high efficacy (89–99%) and can be recommended as a treatment method that provides effective control of CPB, improved economic results, and a positive environmental impact. Based on the advantages that these treatments offer compared to chemical insecticides, further work is recommended to determine if these combinational treatments used in other dosages or in combinations with some other ecologically acceptable insecticides can provide satisfactory control of CPB.

First Sprayable Double-Stranded RNA-Based Biopesticide Product Targets Proteasome Subunit Beta Type-5 in Colorado Potato Beetle (Leptinotarsa decemlineata)

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a sprayable double-stranded RNA biopesticide with a new mode of action that triggers the RNA interference pathway. Laboratory assays with second instar larvae fed Ledprona showed a dose–response where 25×10−6g/L of dsPSMB5 caused 90% mortality after 6days of initial exposure. We also showed that exposure to Ledprona for 6h caused larval mortality and decreased target messenger RNA (mRNA) expression. Decrease in PSMB5 protein levels was observed after 48h of larval exposure to Ledprona. Both PSMB5 mRNA and protein levels did not recover over time. Ledprona efficacy was demonstrated in a whole plant greenhouse trial and performed similarly to spinosad. Ledprona, currently pending registration at EPA, represents a new biopesticide class integrated pest management and insecticide resistance management programs directed against CPB.

Efficacy of the Applied Natural Enemies on the Survival of Colorado Potato Beetle Adults

Colorado potato beetle Leptinotarsa decemlineata is among the most destructive pests of potatoes quickly developing resistance to traditional insecticides. In the present study, we tested the effect of various species and strains of entomopathogenic nematodes on CPB adults, and subsequently, the most effective nematodes were applied alone and in combination with entomopathogenic fungus B. bassiana in pots with potato plants and in the field and their effect on the number of emerging adults was evaluated. In the experimental infections, both the nematode invasion and pathogenicity were variable, and, in several strains, the mortality reached 100%. In pot experiments, soil application of nematodes S. carpocapsae 1343 and S. feltiae Jakub and fungus significantly decreased numbers of emerging CPB adults, while, after the application on leaves, only fungal treatment was effective. The field application of fungus B. bassiana significantly decreased the number of emerging CPB adults in comparison to control sites by ca. 30% while the effect of nematodes and the nematodes–fungus combination was not significant. In conclusion, we demonstrate the necessity of thorough bioassays to select the most effective nematode strains. Entomopathogenic nematodes have the potential to effectively decrease the emergence of CPB adults, but further research is needed to improve the effectiveness in the field.