scholarly journals The Metabolism of Chromosomal Ribonucleic Acid in Drosophila Salivary Glands and its Relation to Synthesis of Desoxyribonucleic Acid

1959 ◽  
Vol 5 (3) ◽  
pp. 461-467 ◽  
Author(s):  
Rachel McMaster-Kaye ◽  
J. Herbert Taylor
Keyword(s):  
Nucleic Acids ◽  
Salivary Glands ◽  
Ribonucleic Acid ◽  
Time Course ◽  
Marked Decrease ◽  
Morphological Differentiation ◽  
Nuclear Size ◽  
Large Decrease ◽  
Desoxyribonucleic Acid ◽  
Autoradiographic Technique

Incorporation of adenine-8-C14 into chromosomal nucleic acids of Drosophila salivary glands has been observed with the autoradiographic technique. RNA-C14 and DNA-C14 were detected as the fractions extractable by ribonuclease digestion and resistant to ribonuclease, respectively. Extractions with desoxyribonuclease and acids were also used to identify the nucleic acids. Time-course curves were determined from grain counts. RNA-C14 concentration reached a maximum in 2 hours, and decreased after the 4th hour. DNA-C14 concentration reached its maximum within 8 hours, and showed no decreases during a 48-hour experiment. In the latter part of the period of observation, morphological differentiation of the gland occurred, the decrease in RNA-C14 became very rapid, and a large increase in DNA-C14 was observed. Marked decrease in RNA-C14 and increase in DNA-C14 were detectable in a few hours when isotope was administered shortly before visible differentiation of the gland. Measurements of nuclear size indicated no significant decreases in RNA-C14 amount prior to the period of differentiation. During this later period, a large decrease in RNA-C14 amount occurs suddenly, and the same amount of C14 is added simultaneously to the DNA fraction, as expected if RNA-C14 is utilized in the synthesis of DNA.

scholarly journals THE BEHAVIOR OF THE NUCLEIC ACIDS DURING THE EARLY DEVELOPMENT OF THE SEA URCHIN EGG (ARBACIA)

1948 ◽  
Vol 31 (5) ◽  
pp. 457-457
Author(s):  
Gerhard Schmidt ◽  
Liselotte Hecht ◽  
S. J. Thannhauser
Keyword(s):  
Nucleic Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
P Fractions ◽  
Desoxyribonucleic Acid ◽  
Sea Urchin Egg

The authors wish to correct an error in the paper "The behavior of the nucleic acids during the early development of the sea urchin egg (Arbacia)" (J. Gen. Physiol., 1947–48, 31, 203). Owing to an oversight, the figures for the amounts of various P fractions in a single Arbacia egg have been erroneously expressed in γ x 10–3 units (Tables I and II, page 205; the last two lines of page 206). The figures should have been expressed in γ x 10–5 units. Thus, the fertilized Arbacia egg contains an average of 20 γ x 10–5 ribonucleic acid P and 0.7 to 1 γ x 10–5 desoxyribonucleic acid P.

scholarly journals THE BEHAVIOR OF THE NUCLEIC ACIDS DURING THE EARLY DEVELOPMENT OF THE SEA URCHIN EGG (ARBACIA)

1948 ◽  
Vol 31 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Gerhard Schmidt ◽  
Liselotte Hecht ◽  
S. J. Thannhauser
Keyword(s):  
Nucleic Acids ◽  
Early Development ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Desoxyribonucleic Acid ◽  
Sea Urchin Egg

1. The unfertilized Arbacia egg contains an average of 20γ x 10–3 ribonucleic acid and 0.7 to 1γ x 10–3 desoxyribonucleic acid. 2. During the first 24 hours of development, the amount of ribonucleic acid per embryo remains practically unchanged whereas that of desoxyribonucleic acid steadily increases. At the end of this period, the amount of desoxyribonucleic acid per embryo is 10 to 15 times larger than that of the unfertilized egg.

THE EFFECT OF GONADAL HORMONES ON LIVER NUCLEIC ACIDS IN THE IMMATURE PULLET

1949 ◽  
Vol 27d (4) ◽  
pp. 200-206 ◽  
Author(s):  
D. G. Chapman ◽  
A. A. Hanson ◽  
R. H. Common ◽  
W. A. Maw
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Testosterone Propionate ◽  
Gonadal Hormones ◽  
Gonadal Hormone ◽  
Desoxyribonucleic Acid

The ribonucleic acid, desoxyribonucleic acid, and the ratio of ribonucleic acid to desoxyribonucleic acid in the liver of immature pullets treated with 4.5 mgm. testosterone propionate over a 12-day period were all increased when this treatment was supplemented by treatment with estrogen. The possible significance of these effects in relation to other effects of similar gonadal hormone treatments is discussed.

METABOLISM OF LEPTOSPIRES: II. THE ACTION OF 8-AZAGUANINE

1967 ◽  
Vol 13 (12) ◽  
pp. 1621-1629 ◽  
Author(s):  
Russell C. Johnson ◽  
Palmer Rogers
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Inhibitory Effect ◽  
Relative Increase ◽  
Purine Biosynthesis ◽  
Low Concentrations

Both the pathogen Leptospira pomona and the saprophyte L. biflexa Patoc I can convert exogenous adenine, guanine, and 8-azaguanine to the corresponding nucleotide and incorporate them into nucleic acids. L. pomona is inhibited by low concentrations of 8-azaguanine (50 μg/ml) and this inhibition is associated with less than a 5% replacement of the ribonucleic acid (RNA) guanine residues by the analogue. Guanine possessed the highest activity for antagonizing the inhibitory effect of 8-azaguanine. The biosynthetic process of L. pomona most affected by the analogue was a relative increase in RNA synthesis. The analogue-resistant L. biflexa incorporated 1/10 as much 8-azaguanine as L. pomona. The higher rate of purine biosynthesis, in addition to the lesser amount of 8-azaguanine incorporated, may account for the analogue resistance of L. biflexa.

Visualization of nutrient translocation in ectomycorrhizal symbioses

Botany ◽  
2014 ◽  
Vol 92 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Bingyun Wu
Keyword(s):  
Phosphoric Acid ◽  
Time Course ◽  
Two Dimensional ◽  
Fixed Carbon ◽  
Nutrient Translocation ◽  
Host Trees ◽  
Ecm Fungi ◽  
Autoradiographic Technique ◽  
Imaging Plates ◽  
Digital Time

Ectomycorrhizal (ECM) fungi receive photosynthetically fixed carbon from the host tree and, in return, supply nutrients such as phosphorus (P) and nitrogen (N) from the soil. An ECM symbiosis system in a two-dimensional, soil-free rhizobox was developed to visualize nutrient translocation during ECM symbioses using a digital, time-course autoradiographic technique with imaging plates. Several studies using 14C and 33P radioisotope tracing experiments are discussed to demonstrate the translocation of 33P-phosphoric acid and photosynthetically fixed carbon between fungi and host trees and between mycelia via mycelia anastomosis. Additionally, novel techniques that can visualize nutrient translocation during mycorrhizal symbioses are discussed.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

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