Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

THE INFLUENCE OF GROWTH HORMONE ON THE NUCLEIC ACID CONTENT OF RAT LIVER AND MUSCLE

1956 ◽  
Vol 13 (4) ◽  
pp. 429-432 ◽  
Author(s):  
BARBARA J. GRAY
Keyword(s):  
Skeletal Muscle ◽  
Growth Hormone ◽  
Nucleic Acid ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Female Rats ◽  
Nucleic Acid Content ◽  
Pituitary Growth Hormone

SUMMARY Adult female rats were treated with purified pituitary growth hormone for 9 days, and the nucleic acid content of the liver and skeletal muscle determined. An increase was observed in the concentration of muscle ribonucleic acid, expressed per gram of nitrogen, as a result of the treatment. There were no changes in the ribonucleic acid concentration of liver. No changes were observed in the concentration of deoxyribonucleic acid in muscle or liver.

scholarly journals Cytological and Biochemical Studies of Anucleolate Xenopus Larvae

1962 ◽  
Vol s3-103 (61) ◽  
pp. 25-35
Author(s):  
H. WALLACE
Keyword(s):  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Content ◽  
Tail Bud ◽  
Lethal Mutant ◽  
Cytoplasmic Rna ◽  
Biochemical Studies ◽  
And Function

A larval lethal mutant of Xenopus laevis lacks true nucleoli but possesses analogous intranuclear organelles, here termed blobs, which are smaller and more numerous than nucleoli. Cytochemical tests reveal that blobs (like nucleoli) contain ribonucleic acid (RNA), arginine, and alkaline phosphatase, but probably no Feulgen-positive material. Anucleolate larvae are deficient in cytoplasmic RNA. By biochemical methods the nucleic acid content of anucleolate embryos is found to be normal at the tail-bud stage, but does not increase after this. By the time they hatch, anucleolate larvae are deficient in both RNA and deoxyribonucleic acid (DNA). The implications of this and related mutations on the formation and function of the nucleolus are considered. The term ‘blob’ is justified in that it would be misleading to regard such organelles as nucleoli produced by normally latent organizers.

Nucleic Acids in Grass and Grass Silage

1988 ◽  
Vol 1988 ◽  
pp. 86-86
Author(s):  
A B McAllan ◽  
G D Braithwaite
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Content ◽  
Ribonucleic Acid ◽  
Protein Fraction ◽  
Dry Matter ◽  
Deoxyribonucleic Acid ◽  
Total N ◽  
Grass Silage ◽  
Microbial N

Little attention has been directed at defining the ‘protein’ fraction of silages. This component is normally estimated by fractionation based on solubility characteristics and under the conditions most commonly used, nucleic acids (ribonucleic acid (RNA) and deoxyribonucleic acid (DNA)) would appear in the protein fraction. Grasses and legumes can contain appreciable amounts of nucleic acids ranging from 11-29 and 19-53 g/kg dry matter respectively (McAllan, 1982). No information is available as to the effects of ensilage on these nucleic acids. Microbes also contain appreciable amounts of nucleic acids which can account for 150-200 gN/kg total-N of the cell and these amounts may vary according to the stage of growth. It has been suggested (Ullrich, 1982) that microbial-N contribution to the total-N content of silage is as much as 220-280 g/kg. Thus the total amount of silage ‘protein-N’ present in the form of nucleic acid-N (from both plant and microbes) could be appreciable leading to a considerable overestimation of the ‘protein’ content of the silage.

scholarly journals Studies on the Nucleic Acids of the Malaria Parasite, Plasmodium Berghei (Vincke & Lips)

1953 ◽  
Vol 6 (2) ◽  
pp. 234 ◽  
Author(s):  
PR Whitfeld
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Acid Content ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Nucleic Acid Content ◽  
Solid Residue ◽  
Parasite Plasmodium

Changes in� the nucleic acid content of the solid residue obtained by haemolysing the blood of mice infected with Plasmodium berghei have been examined. The residue from blood in which 25 per cent. of the red blood cells were parasitized contained 20-25 times as much ribosenucleic acid (RNA) and 12 times as much desoxyribosenucleic acid (DNA) as the residue from uninfected blood.

Some histochemical changes underlying shoot initiation in tobacco callus cultures

1970 ◽  
Vol 48 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Trevor A. Thorpe ◽  
Toshio Murashige
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Histochemical Study ◽  
Callus Cultures ◽  
Tobacco Callus ◽  
Apparent Change ◽  
Shoot Initiation ◽  
Histochemical Changes

A comparative histochemical study of changes in nucleic acids, proteins, and carbohydrates was performed on freeze-substituted samples of tobacco callus, cultured under shoot-forming and non-organ-forming conditions. The DNA (deoxyribonucleic acid) content per cell showed no apparent change, whereas denser staining for RNA (ribonucleic acid) and protein were observed in shoot-forming regions just before and during the initiation of organized structures. The most dramatic finding was the heavy accumulation of starch in shoot-forming tissue; this accumulation preceded any organ formation and was mainly in regions which ultimately gave rise to primordia.

scholarly journals THE RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID CONTENT OF MONONUCLEAR LEUKOCYTES OF DIFFERENT SIZES STUDIED BY ULTRAVIOLET CYTOPHOTOMETRY

1973 ◽  
Vol 21 (7) ◽  
pp. 628-633 ◽  
Author(s):  
ALFREDO MARIANO GARCIA ◽  
PATRICIA A. N. SULLIVAN
Keyword(s):  
Nucleic Acid ◽  
Cell Size ◽  
Dna Content ◽  
Random Error ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Content ◽  
Mononuclear Leukocytes ◽  
Total Nucleic Acid ◽  
Dna And Rna

Rat mononuclears (lymphocytes and monocytes) were studied for total nucleic acid content by means of ultraviolet cytophotometry. Another set was treated with ribonuclease, and deoxyribonucleic acid (DNA) was measured using the same technique. It was found that total nucleic acid content (DNA and RNA) increases linearly with cell size from about 20 units in lymphocytes having 5 µ in diameter up to around 30 units in cells having 12-14 µ in diameter; this is to say, an almost 50% increase for a 6-7-fold enlargement. After ribonuclease treatment, however, the value of the integrated extinction (DNA) tends to remain constant for different cell sizes. A 650% variation in area is accompanied by a DNA change of less than 6%. The differences between treated and nontreated cells are nonsignificant for populations having up to 7.0-7.5 µ in diameter, which implies that small lymphocytes either have a negligible amount of RNA or that the instrument is not sensitive enough to detect it (less than 7% of the DNA content, this figure being the random error of our technique). These differences become highly significant for mononuclears having 8 µ or more in diameter. Therefore, while DNA tends to be constant and independent from cell size, RNA content tends to be harmoniously inconstant, since it is correlated with cell (and nuclear) size and degree of chromatin diffusion.

The nucleic acid content of peripheral nerve in degeneration and activity

1956 ◽  
Vol 144 (917) ◽  
pp. 520-527 ◽  
Keyword(s):  
Nucleic Acid ◽  
Sciatic Nerve ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Contralateral Side ◽  
Small Extent ◽  
Nucleic Acid Content ◽  
Total Nucleic Acid ◽  
Sciatic Nerves ◽  
Stimulation Of

Total nucleic acid (TNA) and deoxyribonucleic acid (DNA) of degenerating and stimulated sciatic nerves of rabbits have been determined. The ratio DNA/TNA in normal nerves was 0·59. In degenerating nerve the total nucleic acid content increased during the first 96 h, though there was little increase in DNA, the increase being presumably mainly in the fraction containing ribonucleic acid (RNA). On the contralateral side the TNA content remained unchanged, but the DNA/TNA ratio decreased to a small extent. After stimulation of the sciatic nerve for 4 h TNA remained unchanged but DNA decreased markedly.

BLOAT INVESTIGATIONS. STUDIES ON SOLUBLE PROTEINS AND NUCLEIC ACIDS IN BLOATING AND NONBLOATING FORAGES

1969 ◽  
Vol 49 (1) ◽  
pp. 69-75 ◽  
Author(s):  
J. M. McArthur ◽  
J. E. Miltimore
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Total Protein ◽  
Acid Content ◽  
Soluble Proteins ◽  
Nucleic Acid Content ◽  
Total Proteins ◽  
Agar Gel ◽  
Forage Mixtures

The soluble proteins in five bloating and six nonbloating forages were separated chromatographically on agar gel columns and 18S protein, the agent causing bloat, was determined in column effluents. Bloating forages contained approximately one-third of total proteins in the form of 18S; nonbloating forages one-sixth or less. Total protein was highest in bloating forages. Nucleic acid content of the two types of forages was not significantly different. Production of nonbloating forage mixtures by mixing forages of high and low 18S protein is discussed.

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