scholarly journals Hydrazinolysis of Nucleic Acids. II. The Formation of Ribo-apyrimidinic Acid from Yeast Ribonucleic Acid

1959 ◽  
Vol 32 (9) ◽  
pp. 926-931 ◽  
Author(s):  
Shosuke Takemura ◽  
Masazumi Miyazaki
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid

METABOLISM OF LEPTOSPIRES: II. THE ACTION OF 8-AZAGUANINE

1967 ◽  
Vol 13 (12) ◽  
pp. 1621-1629 ◽  
Author(s):  
Russell C. Johnson ◽  
Palmer Rogers
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Inhibitory Effect ◽  
Relative Increase ◽  
Purine Biosynthesis ◽  
Low Concentrations

Both the pathogen Leptospira pomona and the saprophyte L. biflexa Patoc I can convert exogenous adenine, guanine, and 8-azaguanine to the corresponding nucleotide and incorporate them into nucleic acids. L. pomona is inhibited by low concentrations of 8-azaguanine (50 μg/ml) and this inhibition is associated with less than a 5% replacement of the ribonucleic acid (RNA) guanine residues by the analogue. Guanine possessed the highest activity for antagonizing the inhibitory effect of 8-azaguanine. The biosynthetic process of L. pomona most affected by the analogue was a relative increase in RNA synthesis. The analogue-resistant L. biflexa incorporated 1/10 as much 8-azaguanine as L. pomona. The higher rate of purine biosynthesis, in addition to the lesser amount of 8-azaguanine incorporated, may account for the analogue resistance of L. biflexa.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

Metabolism of Nucleic Acids and Protein in the Liver, Brain, and Kidney of Female Rats Subjected to Dietary Restriction During the Period of Gestation as well as the Period of Growth, Gestation, and Lactation

1972 ◽  
Vol 50 (8) ◽  
pp. 832-839 ◽  
Author(s):  
Uma Srivastava ◽  
My-Lien Vu ◽  
Suraj Bhargava ◽  
Tapas Goswami
Keyword(s):  
Nucleic Acids ◽  
Protein Content ◽  
Dietary Restriction ◽  
Ribonucleic Acid ◽  
Cellular Growth ◽  
Female Rats ◽  
Diet Restriction ◽  
Organ Growth ◽  
Period Of Gestation ◽  
Number Of Cells

The metabolism of nucleic acids and protein, and. cellular growth, were studied in various organs of female rats subjected to a diet restriction during the period of gestation as well as the period of growth, gestation, and lactation. In general, both body and organ growth were retarded during this restriction, with certain exceptions. For example, only the liver demonstrated a decrease in organ weight when the diet was restricted during the period of gestation only. The number of cells in the various organs decreased in the experimental groups, whereas the cell size was observed to increase. Furthermore it was found that restrictions during the period of growth, gestation, and lactation increased the accumulation of cellular ribonucleic acid and protein content. The metabolism of ribonucleic acid and protein also seemed to have increased significantly.It was concluded that although dietary restriction caused changes in cellular growth, size, and metabolism of various organs these changes were much less marked when the restriction was imposed only during the period of gestation.

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