We demonstrate an etched silicon vapor chamber integrated with a through-silicon via (TSV) for 3D packaging. The Si vapor chamber chip enables low mismatch in the thermal expansion coefficient of a Si-LSI chip and provides a new heat dissipation path for 3D-LSI inter layer cooling. For the first prototype of the vapor chamber, an outside 33-mm × 33-mm chip consisting of a 25-mm × 25-mm area for the vapor chamber, a wick structure 30-μm high, and a vapor passage 100-μm high is developed. In-situ observation of the behavior of the working fluid through the cover glass and heat transfer enhancement is successfully demonstrated. The improvement rate of thermal resistance is 7.1% compared to a test chip without working fluid. Next, the fluid flow of a second vapor chamber prototype consisting of the first prototype integrated with a TSV structure using a Si pillar of 150-μm diameter is investigated. Thermal resistance and droplet observation conducted to evaluate the influence of the TSV. The operation of the vapor chamber is confirmed when a Si pillar is arranged to a coarse pitch of more than 500 μm. A droplet is generated and the vapor passage is partially obstructed. However, the droplet eventually degenerated and the performance of the vapor chamber is maintained. When the Si pillar is arranged to a fine pitch of 200 μm, the entire vapor passage is blocked during the liquid charging process, and no improvement is observed in the thermal resistance of the chip.