High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1072-1083 ◽  
Author(s):  
Huaying Chen ◽  
Jane Sun ◽  
Ernst Wolvetang ◽  
Justin Cooper-White
Keyword(s):  
High Throughput ◽  
Clonal Growth ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Design Development ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Development And Validation

In this paper, the design, development and validation of a novel high throughput microfluidic device enabling both the robust and rapid trapping of 100's to 1000's of single cells and their in situ clonal growth is described.

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

scholarly journals Optimization of microfluidic single cell trapping for long-term on-chip culture

Lab on a Chip ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 857 ◽  
Author(s):  
Stefan Kobel ◽  
Ana Valero ◽  
Jonas Latt ◽  
Philippe Renaud ◽  
Matthias Lutolf
Keyword(s):  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
On Chip

scholarly journals A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4077-4088 ◽  
Author(s):  
Lindong Weng ◽  
Felix Ellett ◽  
Jon Edd ◽  
Keith H. K. Wong ◽  
Korkut Uygun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Permeability ◽  
Single Cells ◽  
Cell Membrane Permeability ◽  
Volumetric Change ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Passive Pumping

A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.

scholarly journals Simultaneous CUT&Tag profiling of the accessible and silenced regulome in single cells

2021 ◽  
Author(s):  
Derek H Janssens ◽  
Dominik J. Otto ◽  
Manu Setty ◽  
Kami Ahmad ◽  
Steven Henikoff
Keyword(s):  
High Resolution ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
High Throughput ◽  
Single Cells ◽  
Regulatory Elements ◽  
Small Samples ◽  
Signal Deconvolution ◽  
Chromatin Profiling

Cleavage Under Targets & Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA Polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms.

Single Cell Stimulation Assay: Microfluidic Substance Delivery to a Laterally Trapped Cell

2011 ◽  
Author(s):  
Kyohei Terao ◽  
Murat Gel ◽  
Atsuhito Okonogi ◽  
Takaaki Suzuki ◽  
Fumikazu Oohira ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Side Wall ◽  
Cell Stimulation ◽  
Cell Trapping ◽  
Cell Responses ◽  
Single Cell Trapping ◽  
Intracellular Response ◽  
Chemical Stimuli ◽  
Simple Flow

We propose a novel cell stimulation device for the analysis of cell responses to chemical stimuli. In order to deliver chemical substances to target single cells, we developed a microfluidic device having microchannels and apertures in the side wall to subject stimuli to laterally trapped cells. The channels were designed to allow simple flow control with single syringe pump. We demonstrated single cell trapping and culturing of pancreatic β cell with the device. To test its feasibility in cell stimulation assay, intracellular response of the cell to glucose stimulation was demonstrated.

High throughput profiling drug response and apoptosis of single polar cells

2020 ◽  
Vol 8 (37) ◽  
pp. 8614-8622
Author(s):  
Xixi Wu ◽  
Shuangshuang Chen ◽  
Qinghua Lu
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Tumor Heterogeneity ◽  
Drug Response ◽  
Cell Trapping ◽  
Single Cell Trapping

The drug response of single polar cells was evaluated via single cell trapping on anisotropic microwells for tumor heterogeneity.

scholarly journals Efficient analysis of a small number of cancer cells at the single-cell level using an electroactive double-well array

Lab on a Chip ◽  
2016 ◽  
Vol 16 (13) ◽  
pp. 2440-2449 ◽  
Author(s):  
Soo Hyeon Kim ◽  
Teruo Fujii
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Single Cells ◽  
Capture Efficiency ◽  
Single Cell Level ◽  
Cell Capture ◽  
Cell Level ◽  
Cell Trapping ◽  
Highly Efficient ◽  
Single Cell Trapping

The electroactive double well-array consists of trap-wells for highly efficient single-cell trapping using dielectrophoresis (cell capture efficiency of 96 ± 3%) and reaction-wells that confine cell lysates for analysis of intracellular materials from single cells.

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