A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

scholarly journals A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4077-4088 ◽  
Author(s):  
Lindong Weng ◽  
Felix Ellett ◽  
Jon Edd ◽  
Keith H. K. Wong ◽  
Korkut Uygun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Permeability ◽  
Single Cells ◽  
Cell Membrane Permeability ◽  
Volumetric Change ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Passive Pumping

A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.

Single Cell Stimulation Assay: Microfluidic Substance Delivery to a Laterally Trapped Cell

2011 ◽  
Author(s):  
Kyohei Terao ◽  
Murat Gel ◽  
Atsuhito Okonogi ◽  
Takaaki Suzuki ◽  
Fumikazu Oohira ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Side Wall ◽  
Cell Stimulation ◽  
Cell Trapping ◽  
Cell Responses ◽  
Single Cell Trapping ◽  
Intracellular Response ◽  
Chemical Stimuli ◽  
Simple Flow

We propose a novel cell stimulation device for the analysis of cell responses to chemical stimuli. In order to deliver chemical substances to target single cells, we developed a microfluidic device having microchannels and apertures in the side wall to subject stimuli to laterally trapped cells. The channels were designed to allow simple flow control with single syringe pump. We demonstrated single cell trapping and culturing of pancreatic β cell with the device. To test its feasibility in cell stimulation assay, intracellular response of the cell to glucose stimulation was demonstrated.

scholarly journals Efficient analysis of a small number of cancer cells at the single-cell level using an electroactive double-well array

Lab on a Chip ◽  
2016 ◽  
Vol 16 (13) ◽  
pp. 2440-2449 ◽  
Author(s):  
Soo Hyeon Kim ◽  
Teruo Fujii
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Single Cells ◽  
Capture Efficiency ◽  
Single Cell Level ◽  
Cell Capture ◽  
Cell Level ◽  
Cell Trapping ◽  
Highly Efficient ◽  
Single Cell Trapping

The electroactive double well-array consists of trap-wells for highly efficient single-cell trapping using dielectrophoresis (cell capture efficiency of 96 ± 3%) and reaction-wells that confine cell lysates for analysis of intracellular materials from single cells.

scholarly journals Erratum: Integrated microfluidic device for single-cell trapping and spectroscopy

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
C. Liberale ◽  
G. Cojoc ◽  
F. Bragheri ◽  
P. Minzioni ◽  
G. Perozziello ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Trapping ◽  
Single Cell Trapping

scholarly journals A microfluidic device enabling high-efficiency single cell trapping

2015 ◽  
Vol 9 (1) ◽  
pp. 014101 ◽  
Author(s):  
D. Jin ◽  
B. Deng ◽  
J. X. Li ◽  
W. Cai ◽  
L. Tu ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Efficiency ◽  
Cell Trapping ◽  
Single Cell Trapping

Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device

2014 ◽  
Vol 190 ◽  
pp. 570-577 ◽  
Author(s):  
Nai-Chin Chen ◽  
Chun-Hong Chen ◽  
Ming-Kun Chen ◽  
Ling-Sheng Jang ◽  
Min-Haw Wang
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Parallel Plate ◽  
Impedance Measurement ◽  
Cell Trapping ◽  
Single Cell Trapping
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