High throughput profiling drug response and apoptosis of single polar cells

2020 ◽  
Vol 8 (37) ◽  
pp. 8614-8622
Author(s):  
Xixi Wu ◽  
Shuangshuang Chen ◽  
Qinghua Lu
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Tumor Heterogeneity ◽  
Drug Response ◽  
Cell Trapping ◽  
Single Cell Trapping

The drug response of single polar cells was evaluated via single cell trapping on anisotropic microwells for tumor heterogeneity.

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

Single cell trapping and stretching in a femtosecond laser fabricated optofluidic chip

2011 ◽  
Author(s):  
L. Ferrara ◽  
F. Bragheri ◽  
P. Minzioni ◽  
I. Cristiani ◽  
K. C. Vishnubhatla ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Optofluidic Chip

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

scholarly journals A High Throughput Micro-Chamber Array Device for Single Cell Clonal Cultivation and Tumor Heterogeneity Analysis

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Feng-Min Shen ◽  
Lian Zhu ◽  
Heng Ye ◽  
Yu-Jun Yang ◽  
Dai-Wen Pang ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Tumor Heterogeneity ◽  
Heterogeneity Analysis
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