scholarly journals Optimization of microfluidic single cell trapping for long-term on-chip culture

Lab on a Chip ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 857 ◽  
Author(s):  
Stefan Kobel ◽  
Ana Valero ◽  
Jonas Latt ◽  
Philippe Renaud ◽  
Matthias Lutolf
Keyword(s):  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
On Chip

Lab-on-chip device for single cell trapping and analysis

2013 ◽  
Vol 16 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Pratikkumar Shah ◽  
Xuena Zhu ◽  
Chunying Chen ◽  
Ye Hu ◽  
Chen-Zhong Li
Keyword(s):  
Single Cell ◽  
Lab On Chip ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
On Chip

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

Single cell trapping and stretching in a femtosecond laser fabricated optofluidic chip

2011 ◽  
Author(s):  
L. Ferrara ◽  
F. Bragheri ◽  
P. Minzioni ◽  
I. Cristiani ◽  
K. C. Vishnubhatla ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Optofluidic Chip

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

Single-cell trapping utilizing negative dielectrophoretic quadrupole and microwell electrodes

2009 ◽  
Vol 24 (12) ◽  
pp. 3637-3644 ◽  
Author(s):  
Ling-Sheng Jang ◽  
Pao-Hua Huang ◽  
Kung-Chieh Lan
Keyword(s):  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping

Microfluidic-based single cell trapping using a combination of stagnation point flow and physical barrier

2016 ◽  
Vol 32 (3) ◽  
pp. 422-429 ◽  
Author(s):  
Miao Yu ◽  
Zongzheng Chen ◽  
Cheng Xiang ◽  
Bo Liu ◽  
Handi Xie ◽  
...  
Keyword(s):  
Single Cell ◽  
Stagnation Point ◽  
Stagnation Point Flow ◽  
Physical Barrier ◽  
Cell Trapping ◽  
Single Cell Trapping

scholarly journals A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability

Lab on a Chip ◽  
2017 ◽  
Vol 17 (23) ◽  
pp. 4077-4088 ◽  
Author(s):  
Lindong Weng ◽  
Felix Ellett ◽  
Jon Edd ◽  
Keith H. K. Wong ◽  
Korkut Uygun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Permeability ◽  
Single Cells ◽  
Cell Membrane Permeability ◽  
Volumetric Change ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Passive Pumping

A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.

High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1072-1083 ◽  
Author(s):  
Huaying Chen ◽  
Jane Sun ◽  
Ernst Wolvetang ◽  
Justin Cooper-White
Keyword(s):  
High Throughput ◽  
Clonal Growth ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Design Development ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Development And Validation

In this paper, the design, development and validation of a novel high throughput microfluidic device enabling both the robust and rapid trapping of 100's to 1000's of single cells and their in situ clonal growth is described.

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