scholarly journals On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2212 ◽  
Author(s):  
Sinwook Park ◽  
Pavithra A. L. Wijethunga ◽  
Hyejin Moon ◽  
Bumsoo Han
Keyword(s):  
Microfluidic Device ◽  
Cryoprotective Agent ◽  
On Chip ◽  
Digital Microfluidic

On-chip organic synthesis enabled using an engine-and-cargo system in an electrowetting-on-dielectric digital microfluidic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3054-3064 ◽  
Author(s):  
Matin Torabinia ◽  
Parham Asgari ◽  
Udaya Sree Dakarapu ◽  
Junha Jeon ◽  
Hyejin Moon
Keyword(s):  
Organic Synthesis ◽  
Chemical Reaction ◽  
Microfluidic Device ◽  
Electrowetting On Dielectric ◽  
On Chip ◽  
Digital Microfluidic

This paper presents a microfluidic chemical reaction using an electrowetting-on-dielectric (EWOD) digital microfluidic device.

Method for Characterization of Passive Mechanical Filtration of Particles in Digital Microfluidic Devices

2014 ◽  
Author(s):  
Peter D. Dunning ◽  
Pierre E. Sullivan ◽  
Michael J. Schertzer
Keyword(s):  
Microfluidic Device ◽  
Vision System ◽  
Low Cost ◽  
Digital Microfluidics ◽  
Microfluidic Devices ◽  
Comb Filter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reaction Site ◽  
Digital Microfluidic

The ability to remove unbound biological material from a reaction site has applications in many biological protocols, such as those used to detect pathogens and biomarkers. One specific application where washing is critical is the Enzyme-Linked ImmunoSorbent Assay (ELISA). This protocol requires multiple washing steps to remove multiple reagents from a reaction site. Previous work has suggested that a passive mechanical comb filter can be used to wash particles in digital microfluidic devices. A method for the characterization of passive mechanical filtration of particles in Digital MicroFluidic (DMF) devices is presented in this work. In recent years there has been increased development of Lab-On-A-Chip (LOAC) devices for the automation and miniaturization of biological protocols. One platform for further research is in digital microfluidics. A digital microfluidic device can control the movement of pico-to nanoliter droplets of fluid using electrical signals without the use of pumps, valves, and channels. As such, fluidic pathways are not hardwired and the path of each droplet can be easily reconfigured. This is advantageous in biological protocols requiring the use of multiple reagents. Fabrication of these devices is relatively straight forward, since fluid manipulation is possible without the use of complex components. This work presents a method to characterize the performance of a digital microfluidic device using passive mechanical supernatant dilution via image analysis using a low cost vision system. The primary metric for performance of the device is particle retention after multiple passes through the filter. Repeatability of the process will be examined by characterizing performance of multiple devices using the same filter geometry. Qualitative data on repeatability and effectiveness of the dilution technique will also be attained by observing the ease with which the droplet disengages from the filter and by measuring the quantity of fluid trapped on the filter after each filtration step.

Analytical Models to Determine the Electric Field Characteristics of a Multi-Electrode Impedimetric Immunosensor in a Digital Microfluidic Device

2014 ◽  
Author(s):  
Steffen O. P. Blume ◽  
Michael J. Schertzer ◽  
Ridha Ben Mrad ◽  
Pierre E. Sullivan
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Electrode Array ◽  
Analytical Models ◽  
Element Analysis ◽  
Numerical Simulation Methods ◽  
Mapping Techniques ◽  
On Chip ◽  
Digital Microfluidic ◽  
Impedimetric Immunosensor

The level of integration of digital microfluidics is continually increasing to include the system path from fluid manipulation and transport, on to reagent preparation, and finally reaction detection. Digital microfluidics therefore has the capability to encompass all steps of common biochemical protocols. Reported here is a set of analytical models for the design of a coplanar interdigitated multi-electrode array to be used as an impedimetric immunosensor in a digital microfluidic device for on-chip chemical reaction detection. The models are based on conformal mapping techniques, and are compared to results obtained from finite element analysis to discuss limitations of the model. The analytical models are feasible and inexpensive surrogates for numerical simulation methods.

Characterization of size-dependent mechanical properties of tip-growing cells using a lab-on-chip device

Lab on a Chip ◽  
2017 ◽  
Vol 17 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Chengzhi Hu ◽  
Gautam Munglani ◽  
Hannes Vogler ◽  
Tohnyui Ndinyanka Fabrice ◽  
Naveen Shamsudhin ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Wall ◽  
Young’S Modulus ◽  
Microfluidic Device ◽  
Young's Modulus ◽  
Turgor Pressure ◽  
Lab On Chip ◽  
Size Dependent ◽  
On Chip

A microfluidic device can trap and indent tip-growing cells for quantification of turgor pressure and cell wall Young's modulus.

scholarly journals A microfluidic perfusion approach for on-chip characterization of the transport properties of human oocytes

Lab on a Chip ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1297-1305 ◽  
Author(s):  
Gang Zhao ◽  
Zhiguo Zhang ◽  
Yuntian Zhang ◽  
Zhongrong Chen ◽  
Dan Niu ◽  
...  
Keyword(s):  
Transport Properties ◽  
Microfluidic Device ◽  
Osmotic Shock ◽  
Human Oocytes ◽  
Microfluidic Perfusion ◽  
On Chip

A novel sandwich structured microfluidic device based approach that allows for on-chip characterization of the transport properties of human oocytes under innocuous osmotic shock or injury to the cells.

scholarly journals Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes

Lab on a Chip ◽  
2014 ◽  
Vol 14 (11) ◽  
pp. 1891-1900 ◽  
Author(s):  
Shailender Singh Kanwar ◽  
Christopher James Dunlay ◽  
Diane M. Simeone ◽  
Sunitha Nagrath
Keyword(s):  
Clinical Application ◽  
Microfluidic Device ◽  
On Chip

Microfluidic all-in-one device “ExoChip” for isolation, quantification and analysis of circulatory exosomes: bridging the gap between technology and clinical application.

Direct loading of blood for plasma separation and diagnostic assays on a digital microfluidic device

Lab on a Chip ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Christopher Dixon ◽  
Julian Lamanna ◽  
Aaron R. Wheeler
Keyword(s):  
Microfluidic Device ◽  
Porous Membranes ◽  
Plasma Separation ◽  
Diagnostic Assays ◽  
Direct Loading ◽  
On Chip ◽  
Finger Stick ◽  
Digital Microfluidic ◽  
Free Plasma ◽  
Chip Analysis

The integration of 3D porous membranes in a digital microfluidic device enables the generation of cell-free plasma from finger-stick volumes of blood with in-line, on-chip analysis.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Demonstration of an integrated nanophotonic chip-scale alkali vapor magnetometer using inverse design

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Yoel Sebbag ◽  
Eliran Talker ◽  
Alex Naiman ◽  
Yefim Barash ◽  
Uriel Levy
Keyword(s):  
Spatial Resolution ◽  
Near Field ◽  
Computer Assisted ◽  
Experimental Characterization ◽  
Optical Isolation ◽  
New Concepts ◽  
On Chip ◽  
The Cost ◽  
Micrometer Scale

AbstractRecently, there has been growing interest in the miniaturization and integration of atomic-based quantum technologies. In addition to the obvious advantages brought by such integration in facilitating mass production, reducing the footprint, and reducing the cost, the flexibility offered by on-chip integration enables the development of new concepts and capabilities. In particular, recent advanced techniques based on computer-assisted optimization algorithms enable the development of newly engineered photonic structures with unconventional functionalities. Taking this concept further, we hereby demonstrate the design, fabrication, and experimental characterization of an integrated nanophotonic-atomic chip magnetometer based on alkali vapor with a micrometer-scale spatial resolution and a magnetic sensitivity of 700 pT/√Hz. The presented platform paves the way for future applications using integrated photonic–atomic chips, including high-spatial-resolution magnetometry, near-field vectorial imaging, magnetically induced switching, and optical isolation.

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