Analytical Models to Determine the Electric Field Characteristics of a Multi-Electrode Impedimetric Immunosensor in a Digital Microfluidic Device

2014 ◽  
Author(s):  
Steffen O. P. Blume ◽  
Michael J. Schertzer ◽  
Ridha Ben Mrad ◽  
Pierre E. Sullivan
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Electrode Array ◽  
Analytical Models ◽  
Element Analysis ◽  
Numerical Simulation Methods ◽  
Mapping Techniques ◽  
On Chip ◽  
Digital Microfluidic ◽  
Impedimetric Immunosensor

The level of integration of digital microfluidics is continually increasing to include the system path from fluid manipulation and transport, on to reagent preparation, and finally reaction detection. Digital microfluidics therefore has the capability to encompass all steps of common biochemical protocols. Reported here is a set of analytical models for the design of a coplanar interdigitated multi-electrode array to be used as an impedimetric immunosensor in a digital microfluidic device for on-chip chemical reaction detection. The models are based on conformal mapping techniques, and are compared to results obtained from finite element analysis to discuss limitations of the model. The analytical models are feasible and inexpensive surrogates for numerical simulation methods.

A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays

Lab on a Chip ◽  
2015 ◽  
Vol 15 (18) ◽  
pp. 3776-3784 ◽  
Author(s):  
Darius G. Rackus ◽  
Michael D. M. Dryden ◽  
Julian Lamanna ◽  
Alexandre Zaragoza ◽  
Brian Lam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Digital Microfluidic ◽  
Electrochemical Immunoassays

Nanostructured microelectrodes (NMEs) combined with digital microfluidics (DMF) for automated electroimmunoassays.

scholarly journals Interfacing Digital Microfluidics with Ambient Mass Spectrometry Using SU-8 as Dielectric Layer

Micromachines ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 649 ◽  
Author(s):  
Gowtham Sathyanarayanan ◽  
Markus Haapala ◽  
Tiina Sikanen
Keyword(s):  
Mass Spectrometry ◽  
Dielectric Layer ◽  
Digital Microfluidics ◽  
Drug Distribution ◽  
Substrate Material ◽  
Ambient Mass Spectrometry ◽  
Electrowetting On Dielectric ◽  
On Chip ◽  
Ambient Ms ◽  
Digital Microfluidic

This work describes the interfacing of electrowetting-on-dielectric based digital microfluidic (DMF) sample preparation devices with ambient mass spectrometry (MS) via desorption atmospheric pressure photoionization (DAPPI). The DMF droplet manipulation technique was adopted to facilitate drug distribution and metabolism assays in droplet scale, while ambient mass spectrometry (MS) was exploited for the analysis of dried samples directly on the surface of the DMF device. Although ambient MS is well-established for bio- and forensic analyses directly on surfaces, its interfacing with DMF is scarce and requires careful optimization of the surface-sensitive processes, such as sample precipitation and the subsequent desorption/ionization. These technical challenges were addressed and resolved in this study by making use of the high mechanical, thermal, and chemical stability of SU-8. In our assay design, SU-8 served as the dielectric layer for DMF as well as the substrate material for DAPPI-MS. The feasibility of SU-8 based DMF devices for DAPPI-MS was demonstrated in the analysis of selected pharmaceuticals following on-chip liquid-liquid extraction or an enzymatic dealkylation reaction. The lower limits of detection were in the range of 1–10 pmol per droplet (0.25–1.0 µg/mL) for all pharmaceuticals tested.

On-chip organic synthesis enabled using an engine-and-cargo system in an electrowetting-on-dielectric digital microfluidic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3054-3064 ◽  
Author(s):  
Matin Torabinia ◽  
Parham Asgari ◽  
Udaya Sree Dakarapu ◽  
Junha Jeon ◽  
Hyejin Moon
Keyword(s):  
Organic Synthesis ◽  
Chemical Reaction ◽  
Microfluidic Device ◽  
Electrowetting On Dielectric ◽  
On Chip ◽  
Digital Microfluidic

This paper presents a microfluidic chemical reaction using an electrowetting-on-dielectric (EWOD) digital microfluidic device.

A droplet-to-digital (D2D) microfluidic device for single cell assays

Lab on a Chip ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 225-236 ◽  
Author(s):  
Steve C. C. Shih ◽  
Philip C. Gach ◽  
Jess Sustarich ◽  
Blake A. Simmons ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Microfluidic Platform ◽  
Cell Assays ◽  
Digital Microfluidic

We have developed a new hybrid droplet-to-digital microfluidic platform (D2D) that integrates droplet-in-channel microfluidics with digital microfluidics for performing multi-step single cell assays.

Method for Characterization of Passive Mechanical Filtration of Particles in Digital Microfluidic Devices

2014 ◽  
Author(s):  
Peter D. Dunning ◽  
Pierre E. Sullivan ◽  
Michael J. Schertzer
Keyword(s):  
Microfluidic Device ◽  
Vision System ◽  
Low Cost ◽  
Digital Microfluidics ◽  
Microfluidic Devices ◽  
Comb Filter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reaction Site ◽  
Digital Microfluidic

The ability to remove unbound biological material from a reaction site has applications in many biological protocols, such as those used to detect pathogens and biomarkers. One specific application where washing is critical is the Enzyme-Linked ImmunoSorbent Assay (ELISA). This protocol requires multiple washing steps to remove multiple reagents from a reaction site. Previous work has suggested that a passive mechanical comb filter can be used to wash particles in digital microfluidic devices. A method for the characterization of passive mechanical filtration of particles in Digital MicroFluidic (DMF) devices is presented in this work. In recent years there has been increased development of Lab-On-A-Chip (LOAC) devices for the automation and miniaturization of biological protocols. One platform for further research is in digital microfluidics. A digital microfluidic device can control the movement of pico-to nanoliter droplets of fluid using electrical signals without the use of pumps, valves, and channels. As such, fluidic pathways are not hardwired and the path of each droplet can be easily reconfigured. This is advantageous in biological protocols requiring the use of multiple reagents. Fabrication of these devices is relatively straight forward, since fluid manipulation is possible without the use of complex components. This work presents a method to characterize the performance of a digital microfluidic device using passive mechanical supernatant dilution via image analysis using a low cost vision system. The primary metric for performance of the device is particle retention after multiple passes through the filter. Repeatability of the process will be examined by characterizing performance of multiple devices using the same filter geometry. Qualitative data on repeatability and effectiveness of the dilution technique will also be attained by observing the ease with which the droplet disengages from the filter and by measuring the quantity of fluid trapped on the filter after each filtration step.

Liquid-Liquid Extraction Based on Digital Microfluidics

2009 ◽  
Author(s):  
Hyejin Moon ◽  
Praveen Kunchala ◽  
Yasith Nanayakkara ◽  
Daniel W. Armstrong
Keyword(s):  
Room Temperature ◽  
Chemical Engineering ◽  
Digital Microfluidics ◽  
Liquid Extraction ◽  
Extraction Techniques ◽  
Immiscible Liquids ◽  
Liquid Liquid Extraction ◽  
Liquid Phases ◽  
On Chip ◽  
Digital Microfluidic

Liquid-liquid extraction techniques are one of the major tools in chemical engineering, analytical chemistry, and biology, especially in a system where two immiscible liquids have an interface solutes exchange between the two liquid phases along the interface up to a point where the concentration ratios in the two liquids reach their equilibrium values [1]. In this paper, we propose to use room temperature ionic liquid (RTIL) as a second liquid phase for extraction, which forms immiscible interface with aqueous solutions. We demonstrate liquid-liquid extraction with the EWOD digital microfluidic device, two model extraction systems were tested. One is organic dye extracted from RTIL(1-butyl-3-methylimidazolium bis(trifluoromethanesulfonylimide or BMIMNTf2) to water and the other is iodine (I2) extracted from water to BMIMNTf2. Droplets of aqueous solution and BMIMNTf2 solution were generated on chip reservoir then transported for extraction and separated by EWOD actuation successfully.

A Robust Superhydrophobic Surface for Digital Microfluidics

2011 ◽  
Author(s):  
David Barona ◽  
A. Amirfazli
Keyword(s):  
Superhydrophobic Surface ◽  
Mechanical Stability ◽  
Contact Angle Hysteresis ◽  
Digital Microfluidics ◽  
Microfluidic Devices ◽  
Contact Angles ◽  
Lab On Chip ◽  
Adverse Conditions ◽  
On Chip ◽  
Digital Microfluidic

Digital microfluidics depends on efficient movement of individual drops for a variety of tasks, e.g. reagent delivery, mixing, sampling, etc. Superhydrophobic (SH) coatings generally show high repellency and low adhesion for a variety of liquids. Therefore, SH coatings can provide for an efficient drop delivery and hence low energy requirements for a fluidic chip. However, wide application of such coatings is hampered by fragile nature of such coatings to date. A new SH coating is developed that addresses the fragility challenge of such coatings. It is based on application of nanoparticles to fluoropolymers. The mechanical stability, wear resistance and durability under prolonged liquid exposure of this new coating is discussed. It is shown that the new SH coating can maintain high contact angles, low contact angle hysteresis needed for drop mobility under adverse conditions/application of digital microfluidic devices. The developed SH coating can also be sprayed onto various surfaces, including glass used in traditional lab-on-chip (LOC) devices, or even paper for enabling novel Lap-on-paper (LOP) devices.

DNA Ligation on a Digital Microfluidic Device

2020 ◽  
Author(s):  
H. T. An ◽  
S. Houchaimi ◽  
C. T. Burkhart ◽  
M. J. Schertzer
Keyword(s):  
Large Scale ◽  
Silicone Oil ◽  
Digital Microfluidics ◽  
High Volume ◽  
Oligonucleotide Synthesis ◽  
Agricultural Improvement ◽  
Dna Ligation ◽  
Resistive Heater ◽  
On Chip ◽  
Digital Microfluidic

Abstract This investigation demonstrates that digital microfluidic platforms are suitable for automated DNA ligation. Multiple DNA ligation steps are required to create DNA products using oligonucleotide synthesis. Unfortunately, traditional methods of oligonucleotide synthesis are unable to create highly accurate, long DNA products. This leads to a supply-side bottleneck that puts a drag on innovation in drug development, organism engineering, and agricultural improvement. Here we demonstrate ligation of two DNA products into one DNA product in digital microfluidic devices that manipulate droplets in air and in oil. Results from the gel electrophoresis imaging confirmed that ligation on digital microfluidics devices was successful in all cases. Silicone oil experiments also verified that on-chip incubation of DNA ligation is possible on these devices using an external resistive heater. This suggests that large-scale DMF automation of DNA synthesis can be used to alleviate the bottleneck created by the lack of efficient, high-volume production of long change DNA products. Such an advancement would be highly valued for a wide variety of biomedical applications.

Direct loading of blood for plasma separation and diagnostic assays on a digital microfluidic device

Lab on a Chip ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Christopher Dixon ◽  
Julian Lamanna ◽  
Aaron R. Wheeler
Keyword(s):  
Microfluidic Device ◽  
Porous Membranes ◽  
Plasma Separation ◽  
Diagnostic Assays ◽  
Direct Loading ◽  
On Chip ◽  
Finger Stick ◽  
Digital Microfluidic ◽  
Free Plasma ◽  
Chip Analysis

The integration of 3D porous membranes in a digital microfluidic device enables the generation of cell-free plasma from finger-stick volumes of blood with in-line, on-chip analysis.

scholarly journals On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2212 ◽  
Author(s):  
Sinwook Park ◽  
Pavithra A. L. Wijethunga ◽  
Hyejin Moon ◽  
Bumsoo Han
Keyword(s):  
Microfluidic Device ◽  
Cryoprotective Agent ◽  
On Chip ◽  
Digital Microfluidic
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