Plackett–Burman Combined with Box–Behnken to Optimize the Medium of Fermented Tremella Polysaccharide and Compare the Characteristics before and after Optimization

The purpose of this study was to improve the yield of fermented Tremella polysaccharide (FTP) by optimizing the medium and to explore the effect of optimization on the structure. In this paper, the Plackett–Burman design and response surface method were used to optimize the fermentation medium of Tremella spore GT2# for FTP production. The results of the Plackett–Burman design showed that potato extract, glucose, and peptone were the main factors. The Box–Behnken experiment and regression analysis determined that the optimal concentrations of potato extract, glucose, and peptone were 22.01%, 29.38 g/L, and 2.61 g/L, respectively. After optimization, the fermentation period shortened from 5 days to 3 days, and the yield of FTP increased from 10.75 g/L to 12.50 g/L, which was in good agreement with the predicted value (12.36 g/L). Characterization of FTP obtained before and after optimization was carried out, and the results showed that medium optimization had significant effects on the primary structure of FTP, including molecular weight, monosaccharide composition, and proportion. After optimization, the content of acid polysaccharide increased, and the glycoside bonds changed from a-configuration to ß-configuration.

Preparation and α-Glucosidase Inhibitory Activity of Gallic Acid-Dextran Conjugate

Phenolic acid-polysaccharide conjugates, produced in plant food and medicine processing, are thought to account for the α-glucosidase inhibitory activity of the final products. However, this speculation lacks experimental support because of the complexity of the plant system and the polysaccharide structure. In this study, with dextran (average molecular weight, 1000) as the skeleton, a gallic acid-dextran conjugate was synthesized and confirmed by ultraviolet, infrared, and nuclear magnetic resonance spectroscopic analysis for the first time. Furthermore, this gallic acid-dextran conjugate showed inhibition of α-glucosidase due to galloyl groups in a mixed competitive and noncompetitive inhibition mode, whose performance was superior to that of acarbose.