THE FEED ANTIBIOTIC AVOPARCIN AND NET UPTAKE OF AMINO ACIDS FROM THE SMALL INTESTINE OF SHEEP

1984 ◽  
Vol 64 (5) ◽  
pp. 134-135 ◽  
Author(s):  
R. C. MacGREGOR ◽  
D. G. ARMSTRONG
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Key Words ◽  
Proximal Duodenum ◽  
Net Uptake

Net uptake of amino acids from the ovine small intestine appeared to be more efficient in the presence of avoparcin, whether infused directly at the proximal duodenum or infused into the rumen. Key words: Sheep, small intestine, avoparcin, amino acids

The effect of oligosaccharides and lactitol on the ileal digestibilities of amino acids, monosaccharides and bacterial populations and metabolites in the small intestine of weanling pigs

1995 ◽  
Vol 75 (1) ◽  
pp. 99-107 ◽  
Author(s):  
V. M. Gabert ◽  
W. C. Sauer ◽  
R. Mosenthin ◽  
M. Schmitz ◽  
F. Ahrens
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Key Words ◽  
Soybean Meal ◽  
Corn Starch ◽  
Period Change ◽  
Distal Ileum ◽  
Weanling Pigs ◽  
Bacterial Populations ◽  
Nutrient Digestion

An experiment was carried out to examine the effects of supplementing diets for weanling pigs with oligosaccharides and LAC on nutrient digestion and bacterial populations and metabolites in the small intestine. Twelve barrows, weaned at 28 d, were fitted with a simple T-cannula at the distal ileum. The BW of the pigs at weaning and at the conclusion of the experiment were 9.1 and 13.8 kg, respectively. The pigs were fed four diets based on barley, wheat and soybean meal according to a two-period change-over design. The diets were formulated to contain 18% CP. Transgalactosylated oligosaccharides (0.2%), GUO (0.2%) and LAC (1%, 4-O-β-D-galactopyranosyl-D-sorbitol) were included at the expense of corn starch. Supplementation with oligosaccharides or LAC had little effect on the apparent ileal digestibilities of AA and monosaccharides. Supplementation of diets with oligosaccharides or LAC did not affect (P > 0.05) the monosaccharide concentrations in ileal digesta except for galactose which was higher (P < 0.05) in digesta from pigs fed the diet supplemented with LAC. The daily ileal output of monosaccharides, pH, ammonia and VFA concentrations, bacterial populations in ileal digesta and incidence of diarrhea were not affected (P > 0.05). Therefore, the supplementation of diets for weanling pigs (9.1–13.8 kg) with oligosaccharides or LAC at these levels does not affect nutrient digestibilities and bacterial populations in the small intestine. Key words: Pigs, oligosaccharides, lactitol, digestibility, bacteria

INFLUENCE OF CORN GRAIN ON AMINO ACIDS SUPPLIED TO AND ABSORBED FROM THE SMALL INTESTINE

1984 ◽  
Vol 64 (5) ◽  
pp. 195-196 ◽  
Author(s):  
R. J. KOMAREK ◽  
R. A. JANDZINSKI
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Key Words ◽  
Essential Amino Acids ◽  
Corn Grain

Increasing corn grain from 40 to 60% of the diet (as fed) of steers increased the essential amino acids supplied to the small intestine by 30% and the absorption by 59%. Increasing corn from 60 to 80% did not change the supplies but increased the digestibility of protein entering the duodenum. Key words: Cattle, small intestine, amino acids, passage, absorption

scholarly journals Digestion and nutrient net fluxes across the rumen, and the mesenteric- and portal-drained viscera in sheep fed with fresh forage twice daily: net balance and dynamic aspects

2003 ◽  
Vol 89 (5) ◽  
pp. 649-666 ◽  
Author(s):  
Didier Rémond ◽  
Laurence Bernard ◽  
Béatrice Chauveau ◽  
Pierre Nozière ◽  
Claude Poncet
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Protein Amino Acid ◽  
Orchard Grass ◽  
Net Uptake ◽  
Flux Measurements ◽  
Net Flux ◽  
Net Fluxes ◽  
Net Release

Digestion and portal net flux of nutrients were studied in sheep fed twice daily with fresh orchard-grass. Digestive flows were measured in six fistulated sheep using the double-marker technique. Three sheep were fitted with catheters and blood-flow probes, allowing nutrient net flux measurements across the portal-drained viscera (PDV), the mesenteric-drained viscera (MDV) and the rumen. Total tract apparent digestion of N was similar to portal net appearance of N, calculated as the sum of free amino acids (FAA), peptide amino acids (PAA), NH3, and urea net fluxes. PAA accounted for 25 % of non-protein amino acid net release across the PDV. With the exception of glycine and glutamate, the small intestine was the main contributor to this PAA net release. The essential amino acid (EAA) apparent disappearance between the duodenum and the ileum was lower than the net appearance of EAA (FAA + PAA) across the MDV. The value of PDV:MDV flux of free EAA was, on average, 78 %. The rumen accounted for 30 % of the net uptake of EAA by the PDV tissues not drained by the mesenteric vein. Rumen net release of acetate, propionate, butyrate, 3-hydroxybutyrate, and lactate accounted for 70, 55, 46, 77 and 52 %, respectively, of their portal net releases. Conversely, the small intestine was a net consumer of arterial acetate and 3-hydroxybutyrate. Dynamic study of nutrient net fluxes across the PDV showed that throughout a feeding cycle, the liver faced a constant flux of amino acids (AA), whereas volatile fatty acid and NH3net fluxes varied in response to the meal. The present study specified, in forage-fed sheep, the partitioning of nutrient net fluxes across the PDV and the role of peptides in portal net release of AA.

INTESTINAL DIGESTION OF PROTEIN IN DAIRY COWS

1984 ◽  
Vol 64 (5) ◽  
pp. 193-194 ◽  
Author(s):  
T. HVELPLUND
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Dairy Cows ◽  
Key Words ◽  
Cotton Seed ◽  
Apparent Digestibility ◽  
Intestinal Digestion ◽  
Soyabean Meal ◽  
Intestinal Digestibility

Apparent digestibility of AA-N in the small intestine of dairy cows fed eight different diets varied between 0.61 and 0.75. As level of intake was increased a significant decrease in digestibility of AA-N was observed. Treating soyabean meal and cotton seed cakes with 0.5% formaldehyde reduced digestibility significantly. Key words: Cattle, intestinal digestibility, nitrogen, amino acids

Flow rates of components in digesta of pigs prepared with re-entrant cannulas in the proximal duodenum and terminal ileum, and fed semipurified, hard wheat, and soft wheat diets

1976 ◽  
Vol 54 (6) ◽  
pp. 891-897 ◽  
Author(s):  
Michael Ivan ◽  
David J. Farrell
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Terminal Ileum ◽  
Peak Flow ◽  
Essential Amino Acids ◽  
Soft Wheat ◽  
Flow Rates ◽  
Hard Wheat ◽  
Proximal Duodenum ◽  
Post Feeding

Four pigs prepared with re-entrant cannulas in the proximal duodenum and terminal ileum were used to study flow rates of total digesta, insoluble dry matter, nitrogen, and amino acids entering and leaving the small intestine. The pigs received a semipurified diet, a hard wheat diet, or a soft wheat diet. These were approximately isonitrogenous.A higher rate of passage of digesta through the proximal duodenum and terminal ileum were measured in pigs receiving the hard wheat diet. Peak flow of digesta at the duodenum of all pigs occurred at 1 h post feeding. Peak flow of digesta at the ileum occurred at 9 h post feeding on the soft wheat diet, but somewhat earlier on the hard wheat and semipurified diet. More nitrogen and essential amino acids flowed in the solid fraction of duodenal digesta during the first 2 h post feeding for the wheat diets and 4 h post feeding for the semipurified diet.It was concluded that flow rate of most nutrients from the stomach and through the small intestine of pigs is modified by the composition and texture of the food ingested. It is postulated that efficiency of mixing of digesta with digestive secretions in the stomach is a major factor influencing rate of flow.

PREDICTION OF AMINO ACID SUPPLY TO THE SMALL INTESTINE OF COWS FROM ANALYSIS OF THE FEED

1984 ◽  
Vol 64 (5) ◽  
pp. 86-88 ◽  
Author(s):  
JØRGEN MADSEN ◽  
TORBEN HVELPLUND
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Key Words ◽  
Microbial Synthesis ◽  
Lactating Cows

Thirty three different diets were fed to duodenal fistulated lactating cows and the amino acid passage at duodenum was measured. It was found that the amino acid passage could be estimated from the amount of total digested carbohydrates plus the degradability of the proteins in the single feeds. Key words: Amino acids, degradability, microbial synthesis, cattle

DIGESTION OF NUTRIENTS IN THE SMALL INTESTINE OF PIGS FED DIETS CONTAINING RAW AND AUTOCLAVED FABA BEANS

1976 ◽  
Vol 56 (3) ◽  
pp. 451-456 ◽  
Author(s):  
M. IVAN ◽  
J. P. BOWLAND
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Beneficial Effect ◽  
Dietary Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Sole Source ◽  
Intestinal Uptake ◽  
Gross Energy ◽  
Faba Beans

Four castrated pigs, each fitted with a re-entrant cannula in the terminal ileum, were used to study digestion in the small intestine. A nitrogen-free diet was used for the estimation of metabolic nitrogen and amino acids. Faba beans, as the sole source of dietary protein, were used raw or after autoclaving for 30 or 60 min. The four diets were fed to the pigs in a 4 × 4 latin square experiment. The pigs were fed each diet for 6 days prior to a 24-hr collection of total ileal contents. Autoclaving of faba beans had no significant effect on digestibility of dry matter, gross energy, nitrogen and individual amino acids except arginine, which was significantly increased. The intestinal uptake of arginine was the highest and of cystine the lowest in all faba bean diets. It was concluded that autoclaving faba beans had no beneficial effect on the digestion of nutrients in the small intestine of the pig.

Effect of Intraduodenal Starch Infusion on Net Portal Absorption of Amino Acids in Growing steers Fed Lucerne

1994 ◽  
Vol 1994 ◽  
pp. 28-28
Author(s):  
C.J. Seal ◽  
D.S. Parker ◽  
J.C. MacRae ◽  
G.E. Lobley
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Gastrointestinal Tract ◽  
Protein Turnover ◽  
Portal Blood ◽  
Intestinal Lumen ◽  
Short Term ◽  
Net Uptake ◽  
Amino Acid Requirements ◽  
Glucose Availability

Amino acid requirements for energy metabolism and protein turnover within the gastrointestinal tract are substantial and may be met from luminal and arterial pools of amino acids. Several studies have demonstrated that the quantity of amino acids appearing in the portal blood does not balance apparent disappearance from the intestinal lumen and that changing diet or the availability of energy-yielding substrates to the gut tissues may influence the uptake of amino acids into the portal blood (Seal & Reynolds, 1993). For example, increased net absorption of amino acids was observed in animals receiving exogenous intraruminal propionate (Seal & Parker, 1991) and this was accompanied by changes in glucose utilisation by the gut tissues. In contrast, there was no apparent change in net uptake of [l-13C]-leucine into the portal vein of sheep receiving short term intraduodenal infusions of glucose (Piccioli Cappelli et al, 1993). This experiment was designed to further investigate the effects on amino acid absorption of changing glucose availability to the gut with short term (seven hours) or prolonged (three days) exposure to starch infused directly into the duodenum.

Measurement of protein turnover in the small intestine of lambs. 2. Effects of feed intake

1997 ◽  
Vol 128 (2) ◽  
pp. 233-246 ◽  
Author(s):  
S. A. NEUTZE ◽  
J. M. GOODEN ◽  
V. H. ODDY
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Small Intestine ◽  
Experimental Model ◽  
Feed Intake ◽  
Protein Turnover ◽  
Jugular Vein ◽  
Specific Radioactivity ◽  
Whole Body ◽  
Body Protein

This study used an experimental model, described in a companion paper, to examine the effects of feed intake on protein turnover in the small intestine of lambs. Ten male castrate lambs (∼ 10 months old) were offered, via continuous feeders, either 400 (n = 5) or 1200 (n = 5) g/day lucerne chaff, and mean experimental liveweights were 28 and 33 kg respectively. All lambs were prepared with catheters in the cranial mesenteric vein (CMV), femoral artery (FA), jugular vein and abomasum, and a blood flow probe around the CMV. Cr-EDTA (0·139 mg Cr/ml, ∼ 0·2 ml/min) was infused abomasally for 24 h and L-[2,6-3H]phenylalanine (Phe) (420±9·35 μCi into the abomasum) and L-[U-14C]phenylalanine (49·6±3·59 μCi into the jugular vein) were also infused during the last 8 h. Blood from the CMV and FA was sampled during the isotope infusions. At the end of infusions, lambs were killed and tissue (n = 4) and digesta (n = 2) samples removed from the small intestine (SI) of each animal. Transfers of labelled and unlabelled Phe were measured between SI tissue, its lumen and blood, enabling both fractional and absolute rates of protein synthesis and gain to be estimated.Total SI mass increased significantly with feed intake (P < 0·05), although not on a liveweight basis. Fractional rates of protein gain in the SI tended to increase (P = 0·12) with feed intake; these rates were −16·2 (±13·7) and 23·3 (±15·2) % per day in lambs offered 400 and 1200 g/day respectively. Mean protein synthesis and fractional synthesis rates (FSR), calculated from the mean retention of 14C and 3H in SI tissue, were both positively affected by feed intake (0·01 < P < 0·05). The choice of free Phe pool for estimating precursor specific radioactivity (SRA) for protein synthesis had a major effect on FSR. Assuming that tissue free Phe SRA represented precursor SRA, mean FSR were 81 (±15) and 145 (±24) % per day in lambs offered 400 and 1200 g/day respectively. Corresponding estimates for free Phe SRA in the FA and CMV were 28 (±2·9) and 42 (±3·5) % per day on 400 g/day, and 61 (±2·9) and 94 (±6·0) on 1200 g/day. The correct value for protein synthesis was therefore in doubt, although indirect evidence suggested that blood SRA (either FA or CMV) may be closest to true precursor SRA. This evidence included (i) comparison with flooding dose estimates of FSR, (ii) comparison of 3H[ratio ]14C Phe SRA in free Phe pools with this ratio in SI protein, and (iii) the proportion of SI energy use associated with protein synthesis.Using the experimental model, the proportion of small intestinal protein synthesis exported was estimated as 0·13–0·27 (depending on the choice of precursor) and was unaffected by feed intake. The contribution of the small intestine to whole body protein synthesis tended to be higher in lambs offered 1200 g/day (0·21) than in those offered 400 g/day (0·13). The data obtained in this study suggested a role for the small intestine in modulating amino acid supply with changes in feed intake. At high intake (1200 g/day), the small intestine increases in mass and CMV uptake of amino acids is less than absorption from the lumen, while at low intake (400 g/day), this organ loses mass and CMV uptake of amino acids exceeds that absorbed. The implications of these findings are discussed.

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