Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

Keratinase Production by Endophytic Bacteria Aneurinibacillus aneurinilyticus VRCS-4 Isolated from Xerophytic Plant Opuntia ficus - indica (Prickly pear)

Bacterial endophytes colonize an ecological niche which is unexplored site makes them suitable to produce pharmacologically active substances with vast biotechnological potential therefore, xerophytes were chosen to isolate the endophytes. In the present study forty endophytic bacterial isolates were isolated from xerophytic plants grown near poultry farms and feather dumping sites. Of them eight isolates showed zone of hydrolysis and the maximum zone of hydrolyisis of 36mm was with VRCS-4 on skimmed milk agar. This isolate exhibited efficient feather degradation and was identified as Aneurinibacillus aneurinilyticus based on its morphological, biochemical test and molecular sequencing method. The isolate was deposited in NCBI with an accession number MW227423.The isolate showed maximum en-zyme activity of 140.24U/ml at 72h, pH 7.5 and 40º C at 140 rpm. Chicken feather 1% (w/v) used as a sole source of carbon and nitrogen. Feather deg-radation by A.aneurinilyticus VRCS-4 showed 90% degradation in feather meal broth. Ours appears to be the first report on keratinase production by endophytic bacteria from xerophytic plant (Opuntia ficus -indica).

Legal Pluralism and Science and Technology Studies: Exploring Sources of the Legal Pluriverse

In introducing the contributions to this special section, we explore the links between social and juridical concepts of normativity and science and technology. We follow the Legal Pluralism challenge to the notion of state law as the sole source of normative order and point to how technological transformation creates a pluralistic legal universe that takes on new shapes under conditions of globalization. We promote a science and technology studies (STS)-inspired reworking of Legal Pluralism and suggest expanding the portfolio of legally effective regimes of ordering to include the normativity generated by materiality and technology. This normativity is amply demonstrated in the case studies included in the papers which make up this special section. We conclude that the inclusion of approaches developed in STS research helps analytically to overcome what we view as an incomplete law project, one unable to deal with the technicized lifeworlds of a global modernity. The contributions to this special section illustrate that technomaterial change cannot be understood without recognition of the role of normative impacts, and conversely, the legal pluriverse cannot be understood without recognition of the normative role of techno-material arrangements.

Characterization of Aniline Degradation by A Previously Isolated Molybdenum-reducing Pseudomonas sp.

Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 μL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.

SEMANTIC PROSODY AND PREFERENCE OF “HEALTHY” AND “UNHEALTHY” COLLOCATIONS IN COVID-19 CORPUS

This study is conducted in order to know the collocations of ‘healthy’ and ‘unhealthy’ as well as to explore the lexical meaning of those collocations. Corpus-based approach is employed in this study since the sole source of the data is the corpus data. Qualitative research method is used in order to find the hypotheses from the corpus data which is taken from Sketch Engine. The results demonstrate that the collocations of two node words are dissimilar in the categorization. ‘healthy’ node word indicates that three major semantic preferences are associated with it - human, animal, disease. On the contrary, the semantic preferences of ‘unhealthy’ node word are diverse. Thus, the classification is based on the meaning of the collocations. The collocations with negative meaning occur more frequently than those with positive meaning. It is due to the fact that they use the prefixes –in and –un which create the opposite meaning of the original word. Therefore, the negative semantic prosody is more frequently found the two node words – ‘healthy’ and ‘unhealthy’.

An Alternate Approach to Military Rations for Optimal Health and Performance

ABSTRACT Introduction Operational ration packs are the sole source of nutrition when military personnel cannot access fresh food and field kitchens due to deployment and training in remote and hostile locations. They should be light, durable, nutrient rich, and contain sufficient energy to ensure that the personnel can carry out the expected duties. The macronutrient composition of rations has remained relatively unchanged despite escalating concerns related to the health and operational readiness of personnel globally. Currently, the New Zealand Defence Force (NZDF) provides the personnel with a 24-hour ration pack. The aims of this study were to (1) analyse the nutrient content, cost, and weight of the NZDF-supplied ration pack and (2) develop and analyse an alternate ration pack. The alternate ration pack was designed with the intention of improving overall quality and macronutrient distribution ratio, to align with optimal health and performance outcomes. Materials and Methods Nutrient and weight analyses of the NZDF and alternate ration packs were conducted using nutrition analysis software Foodworks V. 10 (Xyris software). The ration packs were costed using information from the NZDF and from commercial online shopping websites (particularly Countdown supermarket and an online shop, iHerb). Data from nutrition panels were entered into Foodworks V. 10 (Xyris software). The data underlying this article will be shared on reasonable request to the corresponding author. Results The NZDF-supplied ration pack cost 37.00 NZD and contained an excessive amount of sugar (636 g or 46% total energy) and marginally insufficient protein (118.7 g or 9% total energy) to sustain physically active military personnel. Comparatively, the alternate ration pack was more costly (63.55 NZD) and contained significantly less sugar (74.6 g or 7.2% total energy) and exceeded protein (263.1 g or 26% total energy) requirements for physically active military personnel. Furthermore, the alternate ration pack was significantly lighter (0.71 kg) than the NZDF ration pack (1.4 kg). In summary, the alternate ration was nutritionally superior and lighter when compared to the currently supplied NZDF ration, but more expensive when purchased as a one-off. Conclusions This work highlights the shortcomings of currently supplied military rations packs (i.e., excessive sugar and marginally inadequate protein) and proposes a novel alternate approach to ration pack formulation. This approach would significantly reduce sugar and increase protein and fat content in military rations. Although this work indicates that the alternate approach (which would produce lighter and nutritionally superior rations) is more costly, this cost could be reduced significantly through bulk purchasing and purpose-built rations and food items. Considering these findings, field user-testing of the alternate ration pack is recommended and subsequent reformulation of guidelines for ration pack development, as appropriate.

Enabling Year-round Cultivation in the Nordics-Agrivoltaics and Adaptive LED Lighting Control of Daily Light Integral

High efficacy LED lamps combined with adaptive lighting control and greenhouse integrated photovoltaics (PV) could enable the concept of year-round cultivation. This concept can be especially useful for increasing the production in the Nordic countries of crops like herbaceous perennials, forest seedlings, and other potted plants not native of the region, which are grown more than one season in this harsh climate. Meteorological satellite data of this region was analyzed in a parametric study to evaluate the potential of these technologies. The generated maps showed monthly average temperatures fluctuating from −20 °C to 20 °C throughout the year. The natural photoperiod and light intensity also changed drastically, resulting in monthly average daily light integral (DLI) levels ranging from 45–50 mol·m−2·d−1 in summer and contrasting with 0–5 mol·m−2·d−1 during winter. To compensate, growth room cultivation that is independent of outdoor conditions could be used in winter. Depending on the efficacy of the lamps, the electricity required for sole-source lighting at an intensity of 300 µmol·m−2·s−1 for 16 h would be between 1.4 and 2.4 kWh·m−2·d−1. Greenhouses with supplementary lighting could help start the cultivation earlier in spring and extend it further into autumn. The energy required for lighting highly depends on several factors such as the natural light transmittance, the light threshold settings, and the lighting control protocol, resulting in electric demands between 0.6 and 2.4 kWh·m−2·d−1. Integrating PV on the roof or wall structures of the greenhouse could offset some of this electricity, with specific energy yields ranging from 400 to 1120 kWh·kW−1·yr−1 depending on the region and system design.

Novel Data-Driven Methodology for Production Allocation Calculation Utilizing Physics-Based Models Coupled and Integrated Within a Digital Framework

Determining the production from each well is crucial for financial and technical purposes. Moreover, this production can be anticipated using several different techniques. This paper describes the procedures to calculate the production allocated to each well in a giant gas-producing field by utilizing physics-based models that are orchestrated in a dynamic digital platform to provide a robust and efficient solution. The cases for this study of allocating gas rates to individual wells were performed using a digital platform as the primary tool utilized to account for the main productional location factors such as well tests and events that are used to estimate actual production volumes. Subsequently, relevant data is extracted, filtered, and loaded into the system in a dynamic interaction with fewer human interventions. The methodology for calculating the production allocated followed these main steps: a) Determine production per well under existing possible measures, b) Determine well contribution factors, c) Distribute actual rates and production according to allocation factors. By using polynomial equations where the inflow performance of the gas wells was verified, the allocation rates were calculated at every desired point of the network. Having an integrated platform proved to be advantageous since it provided a seamless link between different relevant manual and real-time databases and well / network models bringing unique capabilities and benefits. While comparing this integrated and holistic approach versus the previously established one, it was highlighted that production allocation using mainly choke sizes and well test as a sole source for well production can bring significant variations. This creates production mismatches at the well level; therefore, it portrays a misrepresentation of the actual field conditions. Numerous challenges, which are usually faced while calculating the production allocation process, were overcome during the development of this study, such as frequent surface network changes, lack of databases communication, and daily variations on the on/off wells’ status. Furthermore, the data management capabilities of the framework allowed data to be quickly accessible by the users whenever needed allowing them to visualize across the different teams and departments, taking actions when and where required. This standardized methodology provided consistency, reliability, and accuracy, which can be replicated on oil-producing fields and networks; it can be enhanced and scaled in order to incorporate other business processes such as well allowable calculation and voidage monitoring.

Polyhydroxyalkanoates (PHAs) Production from Volatile Fatty Acids (VFAs) from Organic Wastes by Pseudomonas oleovorans

This study aimed to investigate the production of polyhydroxyalkanoates (PHAs), a biodegradable polymer from organic wastes by Pseudomonas oleovorans. Volatile fatty acids (VFAs) from acidogenic fermentations of chicken manure (VFAs-CM) and potato peels (VFAs-PP), rich in organic matter majorly acetic (49.9%), butyric (15%) and propionic acids (11.1%) were utilized as substrates for microbial processes. During 72 h of cultivations, samples were withdrawn at intervals and analyzed for cell growth parameters, PHAs accumulation and polymeric properties. The highest biopolymer accumulation (0.39 g PHAs/g DCW) was achieved at 48 h of cultivation from medium containing VFAs-PP as the sole source of carbon. On characterization, the produced biopolymers were shown to be semi-crystalline of carbonyl C=O group. Additionally, thermogravimetric analysis (TGA) showed that the produced biopolymers demonstrated the capability to withstand thermal degradation above prescribed temperatures at which cross-linking isomerization reaction occurs, which is a vital property denoting the thermal stability of biopolymer.