scholarly journals The organization of classical satellite DNAs in human chromosomes: an approach using AluI and TaqI restriction endonucleases

10.4081/829 ◽  
2009 ◽  
Vol 47 (3) ◽  
pp. 209
Author(s):  
M Nieddu ◽  
G Pichiri ◽  
G Diaz ◽  
R Mezzanotte
Keyword(s):  
Restriction Endonucleases ◽  
Human Chromosomes ◽  
Satellite Dnas

Patterns of digestion of human chromosomes by restriction endonucleases demonstrated byin situ nick translation

1990 ◽  
Vol 22 (12) ◽  
pp. 639-652 ◽  
Author(s):  
A. T. Sumner ◽  
M. H. Taggart ◽  
R. Mezzanotte ◽  
L. Ferrucci
Keyword(s):  
Restriction Endonucleases ◽  
Human Chromosomes ◽  
Nick Translation

Cryptic variants of acrocentric human chromosomes as analysed by restriction endonucleases

Genetica ◽  
1991 ◽  
Vol 83 (3) ◽  
pp. 203-206 ◽  
Author(s):  
S. F. De Cabo ◽  
P. Ludeña ◽  
M. Velázquez ◽  
C. Sentis ◽  
J. Fernández-Piqueras
Keyword(s):  
Restriction Endonucleases ◽  
Human Chromosomes

Characterization of human chromosomal constitutive heterochromatin

1986 ◽  
Vol 28 (5) ◽  
pp. 631-644 ◽  
Author(s):  
Arvind Babu ◽  
Ram S. Verma
Keyword(s):  
Dna Sequences ◽  
Constitutive Heterochromatin ◽  
Restriction Endonucleases ◽  
Chromosome 1 ◽  
Human Chromosomes ◽  
Selective Staining ◽  
Distamycin A ◽  
Staining Techniques ◽  
G 11

The constitutive heterochromatin of human chromosomes is evaluated by various selective staining techniques, i.e., CBG, G-11, distamycin A plus 4,6-diamidino-2-phenylindole-2-HCl (DA/DAPI), the fluorochrome D287/170, and Giemsa staining following the treatments with restriction endonucleases AluI and HaeIII. It is suggested that the constitutive heterochromatin could be arbitrarily divided into at least seven types depending on the staining profiles expressed by different regions of C-bands. The pericentromeric C-bands of chromosomes 1, 5, 7, 9, 13–18, and 20–22 consist of more than one type of chromatin, of which chromosome 1 presents the highest degree of heterogeneity. Chromosomes 3 and 4 show relatively less consistent heterogeneous fractions in their C-bands. The C-bands of chromosomes 10, 19, and the Y do not have much heterogeneity but have characteristic patterns with other methods using restriction endonucleases. Chromosomes 2, 6, 8, 11, 12, and X have homogeneous bands stained by the CBG technique only. Among the chromosomes with smaller pericentric C-bands, chromosome 18 shows frequent heteromorphic variants for the size and position (inversions) of the AluI resistant fraction of C-band. The analysis of various types of heterochromatin with respect to specific satellite and nonsatellite DNA sequences suggest that the staining profiles are probably related to sequence diversity.Key words: polymorphism, heteromorphism, heterogeneity, banding, restriction endonucleases.

The action of ultraviolet light on the patterns of banding induced by restriction endonucleases in human chromosomes

Chromosoma ◽  
1984 ◽  
Vol 90 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Nestor O. Bianchi ◽  
Martha S. Bianchi ◽  
James E. Cleaver
Keyword(s):  
Ultraviolet Light ◽  
Restriction Endonucleases ◽  
Human Chromosomes

The location of four human satellite DNAs on human chromosomes

1975 ◽  
Vol 14 (3-6) ◽  
pp. 338-339 ◽  
Author(s):  
J.R. Gosden ◽  
A.R. Mitchell ◽  
R.A. Buckland ◽  
R.P. Clayton ◽  
H.J. Evans
Keyword(s):  
Human Chromosomes ◽  
Satellite Dnas

The effect of restriction enzyme digestion of human metaphase chromosomes on C-band variants of chromosomes 1 and 9

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 652-655 ◽  
Author(s):  
Ute Hedemann ◽  
M. Schürmann ◽  
E. Schwinger
Keyword(s):  
Restriction Enzyme ◽  
Restriction Enzymes ◽  
Enzyme Digestion ◽  
Restriction Endonucleases ◽  
Restriction Enzyme Digestion ◽  
Human Chromosomes ◽  
Metaphase Chromosomes ◽  
Homologous Chromosomes

Human metaphase chromosomes, fixed on slides, have been treated with 8 different restriction endonucleases and 29 combinations of 2 restriction enzymes prior to staining with Giemsa. The endonucleases AluI and DdeI and the combinations AluI + DdeI, AluI + HaeIII, AluI + HinfI, and AluI + MboI have then been used to digest metaphase chromosomes of nine individuals with C-band variants of chromosomes 1 or 9, obtained by the CBG technique. The restriction enzyme resistant chromatin of the paracentromeric regions of chromosomes 1 and 9 has been measured and compared with the corresponding CBG-bands. The size of the enzyme resistant chromatin regions depend upon the type of enzyme(s) used. Treatment with AluI + MboI was the only digestion that acted differently on different chromosome pairs. However, within one pair of homologous chromosomes, all digestions revealed the same variations as conventional C-banding.Key words: C-band variants, heterochromatin, human chromosomes, restriction endonucleases.

TaqI digestion reveals fractions of satellite DNAs on human chromosomes

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 251-254 ◽  
Author(s):  
I. Tagarro ◽  
J. J. González-Aguilera ◽  
A. Ma Fernández-Peralta
Keyword(s):  
Restriction Endonuclease ◽  
Human Chromosomes ◽  
Experimental Conditions ◽  
Satellite Dnas ◽  
Final Reaction ◽  
Dna Loss ◽  
Target Sequences ◽  
Main Chromosome ◽  
Taqi Restriction Endonuclease

Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This digestion consists of an extensive DNA loss in heterochromatin subregions of chromosomes 1, 9, 15, 16, and Y. These regions essentially coincide with those corresponding to the main chromosome locations of satellite II DNA, whose tandem repeated units contain many TaqI target sequences, and some satellite III DNA domains enriched in TaqI sites.Key words: TaqI restriction endonuclease, heterochromatin, satellite DNAs, human chromosomes.

The location of four human satellite DNAs on human chromosomes

1975 ◽  
Vol 92 (1) ◽  
pp. 148-158 ◽  
Author(s):  
J.R. Gosden ◽  
A.R. Mitchell ◽  
R.A. Buckland ◽  
R.P. Clayton ◽  
H.J. Evans
Keyword(s):  
Human Chromosomes ◽  
Satellite Dnas
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