scholarly journals Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

10.3791/55064 ◽  
2017 ◽  
Author(s):  
Kevin L. Hockett ◽  
David A. Baltrus
Keyword(s):  
Soft Agar ◽  
Inhibitory Compounds ◽  
Overlay Technique ◽  
Agar Overlay Technique

scholarly journals Bioguided Fractionation ShowsCassia alataExtract to InhibitStaphylococcus epidermidisandPseudomonas aeruginosaGrowth and Biofilm Formation

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Samuel Takashi Saito ◽  
Danielle da Silva Trentin ◽  
Alexandre José Macedo ◽  
Cristina Pungartnik ◽  
Grace Gosmann ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
High Resolution Mass Spectrometry ◽  
Solid Phase ◽  
Folk Medicine ◽  
Bacterial Biofilm ◽  
Aqueous Leaf Extract ◽  
Overlay Technique ◽  
Agar Overlay Technique

Plant extracts have a long history to be used in folk medicine.Cassia alataextracts are known to exert antibacterial activity but details on compounds and mechanism of action remain poorly explored. We purified and concentrated the aqueous leaf extract ofC. alataby reverse phase-solid phase extraction and screened the resulting CaRP extract for antimicrobial activity. CaRP extract exhibited antimicrobial activity forPseudomonas aeruginosa,Staphylococcus epidermidis,S. aureus, andBacillus subtilis. CaRP also inhibited biofilm formation ofS. epidermidisandP. aeruginosa. Several bacterial growth-inhibiting compounds were detected when CaRP extract was fractionated by TLC chromatography coupled to bioautography agar overlay technique. HPLC chromatography of CaRP extract yielded 20 subfractions that were tested by bioautography for antimicrobial activity againstS. aureusandS. epidermidis. Five bioactive fractions were detected and chemically characterized, using high-resolution mass spectrometry (qTOF-MS/MS). Six compounds from four fractions could be characterized as kaempferol, kaempferol-O-diglucoside, kaempferol-O-glucoside, quercetin-O-glucoside, rhein, and danthron. In theSalmonella/microsome assay CaRP showed weak mutagenicity (MI<3) only in strain TA98, pointing to a frameshift mutation activity. These results indicate thatC. alataleaf extract contains a minimum of 7 compounds with antimicrobial activity and that these together or as single substance are active in preventing formation of bacterial biofilm, indicating potential for therapeutic applications.

The potential of spice oils in the control of mycotoxin producing fungi

2000 ◽  
Author(s):  
◽  
Sarla Juglal
Keyword(s):  
Fusarium Moniliforme ◽  
Food Industry ◽  
Active Component ◽  
Aspergillus Parasiticus ◽  
Clove Oil ◽  
Overlay Technique ◽  
Inhibitory Potential ◽  
Layer Chromatography ◽  
Agar Overlay Technique ◽  
Staple Diet

Spice oils are known to exhibit antifungal activity and therefore have the potential to control mycotoxin production. There is a need in the food industry to find measures to control mycotoxins that are frequently associated with grains that form the staple diet of the majority of the population in South Africa. Clove, cinnamon, oregano, tumeric, eucalyptus, neem, aniseed, mace and nutmeg oils were tested to determine their inhibitory potential against growth of Aspergillus parasiticus and Fusarium moniliforme using the agar overlay technique. Varying concentrations of the spice oils, ranging from 0.1 ppm to 2.0 ppm, were incorporated into broth cultures of A. parasiticus and maize patty cultures ofF. moniliforme. Levels of production of aflatoxins and fumonisin were determined using standard thin layer chromatography and highpressure liquid chromatography methods. In addition, the active component of the spice oils were isolated, characterised and tested. The inhibitory potential of these compounds for field use was tested by incorporating clove oil, whole cloves and ground cloves in samp

Screening for antimicrobial activity of some essential oils by the agar overlay technique

1986 ◽  
Vol 8 (6) ◽  
pp. 289-292 ◽  
Author(s):  
A. M. Janssen ◽  
N. L. J. Chin ◽  
J. J. C. Scheffer ◽  
A. Baerheim Svendsen
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Overlay Technique ◽  
Agar Overlay Technique
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