Screening for antimicrobial activity of some essential oils by the agar overlay technique

1986 ◽  
Vol 8 (6) ◽  
pp. 289-292 ◽  
Author(s):  
A. M. Janssen ◽  
N. L. J. Chin ◽  
J. J. C. Scheffer ◽  
A. Baerheim Svendsen
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Overlay Technique ◽  
Agar Overlay Technique

scholarly journals Bioguided Fractionation ShowsCassia alataExtract to InhibitStaphylococcus epidermidisandPseudomonas aeruginosaGrowth and Biofilm Formation

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Samuel Takashi Saito ◽  
Danielle da Silva Trentin ◽  
Alexandre José Macedo ◽  
Cristina Pungartnik ◽  
Grace Gosmann ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Leaf Extract ◽  
High Resolution Mass Spectrometry ◽  
Solid Phase ◽  
Folk Medicine ◽  
Bacterial Biofilm ◽  
Aqueous Leaf Extract ◽  
Overlay Technique ◽  
Agar Overlay Technique

Plant extracts have a long history to be used in folk medicine.Cassia alataextracts are known to exert antibacterial activity but details on compounds and mechanism of action remain poorly explored. We purified and concentrated the aqueous leaf extract ofC. alataby reverse phase-solid phase extraction and screened the resulting CaRP extract for antimicrobial activity. CaRP extract exhibited antimicrobial activity forPseudomonas aeruginosa,Staphylococcus epidermidis,S. aureus, andBacillus subtilis. CaRP also inhibited biofilm formation ofS. epidermidisandP. aeruginosa. Several bacterial growth-inhibiting compounds were detected when CaRP extract was fractionated by TLC chromatography coupled to bioautography agar overlay technique. HPLC chromatography of CaRP extract yielded 20 subfractions that were tested by bioautography for antimicrobial activity againstS. aureusandS. epidermidis. Five bioactive fractions were detected and chemically characterized, using high-resolution mass spectrometry (qTOF-MS/MS). Six compounds from four fractions could be characterized as kaempferol, kaempferol-O-diglucoside, kaempferol-O-glucoside, quercetin-O-glucoside, rhein, and danthron. In theSalmonella/microsome assay CaRP showed weak mutagenicity (MI<3) only in strain TA98, pointing to a frameshift mutation activity. These results indicate thatC. alataleaf extract contains a minimum of 7 compounds with antimicrobial activity and that these together or as single substance are active in preventing formation of bacterial biofilm, indicating potential for therapeutic applications.

Chemical composition and antimicrobial activity of the essential oils of flowers, leaves and stems of Cotinus coggygria

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Milošević ◽  
N Nićiforović ◽  
V Mihailović ◽  
S Solujić ◽  
N Vuković
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Cotinus Coggygria

Antimicrobial activity of essential oils onto pathogenic microorganisms of interest to dentistry

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
VR Santos ◽  
VA Noronha ◽  
JC Silva ◽  
FF Silva ◽  
TF Machado ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Microorganisms

Chemical composition and antimicrobial activity of essential oils of selected Apiaceous plants growing in Egypt

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
N Khalil ◽  
M Ashour ◽  
S Fikry ◽  
A NaserSingab ◽  
O Salama
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils

Chemical composition and antimicrobial activity of the essential oils of Micromeria thymifolia, M. dalmatica and Satureja cuneifolia

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
N Menković ◽  
G Zdunić ◽  
S Tasić ◽  
M Ristić ◽  
T Stević ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils
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