Use of specific dyes in the detection of antimicrobial compounds from crude plant extracts using a thin layer chromatography agar overlay technique

1995 ◽  
Vol 6 (3) ◽  
pp. 125-129 ◽  
Author(s):  
Geeta Saxena ◽  
Susan Farmer ◽  
G. H. N. Towers ◽  
R. E. W. Hancock
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Antimicrobial Compounds ◽  
Overlay Technique ◽  
Layer Chromatography ◽  
Agar Overlay Technique

Thin-Layer Chromatography for Chlorinated Pesticide Residue Analysis without Cleanup

1964 ◽  
Vol 47 (2) ◽  
pp. 306-310
Author(s):  
H V Morley ◽  
M Chiba
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Residue Analysis ◽  
Rapid Screening ◽  
Pesticide Residue Analysis ◽  
Cleanup Procedure ◽  
Layer Chromatography

Abstract Thin-layer chromatography on silica gel may be used for the determination of chlorinated pesticide residues in some plant extracts without prior cleanup, thus eliminating the possible loss of pesticides during the cleanup procedure. Rapid screening of a large number of samples is thus possible with consequent saving of time and labor. When necessary, thin-layer chromatography may be used as a prior cleanup for gas chromatography.

Sodium Methylate Treatment of Cleaned Up Plant Extracts in Confirmation of Some Pesticide Residues By Gas-Liquid and Thin Layer Chromatography

1968 ◽  
Vol 51 (5) ◽  
pp. 1095-1101
Author(s):  
C E Mendoza ◽  
P J Wales ◽  
H A Mcleod ◽  
W P Mckinley
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Pesticide Residues ◽  
Sodium Methylate ◽  
Heptachlor Epoxide ◽  
Rapid Procedure ◽  
Qualitative Confirmation ◽  
Layer Chromatography

Abstract A rapid procedure that involves GLC-EC and TLC analyses of plant extracts 5 min after treatment with sodium methylate is described for the qualitative confirmation of some pesticide residues. Plant extracts are cleaned up by elution through a carboncellulose column before treatment. The compounds studied were aldrin, captan, carbophenothion, p,p'-DDD, de-hydrochlorinated p,p-DDD, p,p'-DDE, o,p -DDT, p,p -DDT, Diazinon, dieldrin, disulfoton, heptachlor, heptachlor epoxide, malathion, methoxychlor, de-hydrochlorinated methoxychlor, mevinphos, and parathion. The pesticides added to beet, lettuce, pineapple, potato, and wheat eluates were reliably confirmed by GLC-EC or TLC after sodium methylate treatment. The procedure could be used to confirm only some of the pesticides added to extracts of apples, carrots, cauliflower, grapes, and peas because of components in these samples that gave interfering peaks

scholarly journals Thin-layer Chromatography of Plant Extracts. IV. Alkaloids.

1966 ◽  
Vol 20 ◽  
pp. 1588-1592 ◽  
Author(s):  
Eskil Hultin ◽  
J. Kärkkäinen ◽  
E. Haahti ◽  
Bengt von Hofsten ◽  
D. H. Williams ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Layer Chromatography

scholarly journals Thin-layer Chromatography of Plant Extracts. II. Sequential Extraction of Plant Material.

1965 ◽  
Vol 19 ◽  
pp. 588-590
Author(s):  
Eskil Hultin ◽  
Pär Holmberg ◽  
Peter Klæboe ◽  
E. Augdahl ◽  
C. Th Pederson ◽  
...  
Keyword(s):  
Thin Layer ◽  
Sequential Extraction ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Plant Material ◽  
Layer Chromatography

scholarly journals Phytochemical Screening and Thin Layer Chromatography Profiling of Various Extracts of Achyranthes aspera and Cissus quadrangularis

2021 ◽  
Vol 10 (4) ◽  
pp. 225-229
Author(s):  
V. Suresh Kumar ◽  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Solvent System ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Achyranthes Aspera ◽  
Cissus Quadrangularis ◽  
Layer Chromatography

Achyranthes aspera and Cissus quadrangularis are important medicinal plants possessing wide biological activity. The natural products obtained from plants provides an unique opportunity for the development of new drugs but due to their complex nature there is a need to isolate and purify the bioactive compounds from plant extracts by separation techniques. Our earlier findings revealed that methanol extracts of A. aspera and C. quadrangularis and aqueous extract of C. quadrangularis exhibited antibacterial activity. This prompted to take up further research to reveal these plants’ potential valuable phytochemicals and therefore an attempt has been made to separate the phytochemical constituents of methanol and aqueous extracts of A. aspera and C. quadrangularis by thin layer chromatography. Phytochemical screening of both plant extracts revealed more concentrated phytochemicals in methanol extracts than in aqueous extracts. The TLC profiling showed that methanol extract of A. aspera and C. quadrangularis are rich in flavonoids and phenolic compounds. Flavonoids were separated with n-butanol:ethyl acetate:water (1:2:3) solvent system at Rf value of 0.66 and was confirmed by post-development derivatization with 3% boric acid and 10% oxalic acid spray. After derivatization, the flavonoid spot could be further visualized under UV light at 254nm. Phenolic compounds were separated with methanol:water (2:1) solvent system at Rf value of 0.88 and confirmed with 10% ferric chloride spray. From the present study, suitable mobile phase for separation of flavonoids and phenolic compound fraction from methanol and aqueous extracts of both plants and TLC profiling have been developed

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