scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK N-HEKSAN BIJI BUAH LANGSAT (Lansium domesticum Corr) TERHADAP BAKTERI Staphylococus Aureus DAN Klebsiella Pneumoniae

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 748
Author(s):  
Anastasia P.R. Nurhamidin ◽  
Fatimawali Fatimawali ◽  
Irma Antasionasti
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Hexane Extract ◽  
Klebsiella Pneumonia ◽  
Clear Zone ◽  
Zone Diameter ◽  
Activity Inhibition ◽  
Lansium Domesticum

ABSTRACT Langsat fruit seeds (Lansium domesticum Corr) are  part of the plant that is known to be antibacterial. Therefore, exploration of langsat fruit as an antibacterial is needed. The purpose of this study was to determine the antibacterial activity of langsat fruit seeds against Staphylococus aureus and Klebsiella pneumoniae using the disc method and the well method. Langsat fruit seed powder was macerated using the maceration method with n-hexane solvent and tested for antibacterial activity. Inhibition of the n-hexane extract of langsat fruit seeds showed the largest clear zone diameter value given by the lowest concentration of 10% by the disc and sumurun method on the Staphylococus aureus and Klebsiella pneumoniaes bacteria 13.6 mm; 12.6 mm; 13 mm; and 14.6 mm. This shows that the n-hexane extract of langsat fruit seeds has strong antibacterial activity against Staphylococus aureus and Klebsiella pneumonia bacteria both by the disc method and the well method. Keywords: langsat fruit seeds, Klebsiella pneumoniae, disc, pitting method, Staphylococus aureus  ABSTRAKBiji buah langsat (Lansium domesticum Corr) merupakan salah satu bagian tanaman yang telah dikenal sebagai antibakteri. Maka dari itu, eksplorasi buah langsat sebagai antibakteri sangat diperlukan. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri biji buah langsat terhadap bakteri Staphylococus aureus  dan Klebsiella pneumoniae menggunakan metode cakram dan metode sumuran. Serbuk biji buah langsat dimaserasi menggunakan metode maserasi dengan pelarut n-Heksan dan dilakukan uji aktivitas antibakteri. Daya hambat ekstrak n-Heksan biji buah langsat menunjukkan nilai diameter zona bening terbesar diberikan oleh konsentrasi terendah 10% dengan metode cakram (13,6 mm pada bakteri Staphylococus aureus dan 12,6 mm pada bakteri Klebsiella pneumoniae) dan metode sumuran (13 mm pada bakteri Staphylococus aureus dan 14,6 mm pada bakteri Klebsiella pneumoniae). Hal ini menunjukkan bahwa ekstrak n-Heksan biji buah langsat memiliki aktivitas antibakteri yang kuat terhadap bakteri Staphylococus aureus  dan Klebsiella pneumoniae baik dengan metode cakram maupun metode sumuran. Kata Kunci: biji buah langsat,Klebsiella pneumoniae, metode cakram, sumuran, Staphylococus aureus

scholarly journals Antibacterial Effect of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 ON Klebsiella pneumoniae Producting Extended-Spectrum β-Lactamase (ESBL)

2019 ◽  
Vol 1 (1) ◽  
pp. 16-27
Author(s):  
Yuani Setiawati ◽  
Wiwin Retnowati ◽  
Achmad Basori
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Main Concern ◽  
Peak Activity ◽  
Streptomyces Sp ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Extended Spectrum

Klebsiella pneumoniae as an opportunistic pathogen can cause nosocomial infection.  The main concern on this bacterium is directed on the extended-spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae. The therapy of ESBL-producing Klebsiella pneumoniae infections is very limited because of its multidrug resistance. It had been found new local isolates Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 in mangrove East Coast of Surabaya. These isolates have potential to produce antibiotics (Retnowati, 2008). This study was aimed to prove these isolates may inhibit the growth of ESBL-producing Klebsiella pneumonia. The test of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 using the modification Agar print method against clinical isolates of ESBL-producing Klebsiella pneumoniae. The diameter of inhibition zone (mm) formed shows activity of these isolates. The profiles of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 were different in terms of periode required to reach peak activity, duration of activity and inhibition zone diameter produced during 10 days of observation. Streptomyces sp-MWS3 reached the peak activity most rapidly on day 3 with the largest inhibition zone diameter of 9 mm in ESBL-producing Klebsiella pneumoniae. There were significant differences in inhibition zone diameter between Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 against ESBL-producing Klebsiella pneumoniae.

scholarly journals UJI DAYA HAMBAT EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K.Schum) TERHADAP PERTUMBUHAN BAKTERI Klebsiella pneumoniae ISOLAT SPUTUM PADA PENDERITA PNEUMONIA RESISTEN ANTIBIOTIK SEFTRIAKSON

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Poetry Melinda Abubakar ◽  
Fatimawali Fatimawali ◽  
Paulina Yamlean
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Growth ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Alpinia Purpurata ◽  
Inhibition Ability

ABSTRACT Red galangal rhizome is a plant that has various properties including antifungal and antibacterial. Red galangal rhizomes contain a class of flavonoids, phenols and terpenoids, which can be, used as basic ingredients of modern medicines. Flavonoids and phenols are also known to inhibit microbes. Flavonoids can inhibit microbes that have been resistant to antibiotics. This study aims to determine the antibacterial activity of red galangal rhizome extract (Alpinia purpurata K.Schum) and test its inhibitory effect on the growth of Klebsiella pneumoniae bacteria in sputum isolates in patients with pneumonia resistant ceftriaxone antibiotics. Red galangal rhizome plant was extracted by maceration method using 96% ethanol. Antibacterial inhibitory tests were carried out using the disc and well method. The results showed that the red galangal extract of rhizome had a inhibitory effect on the bacterium Klebsiella pneumoniae. On the disc method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone is 9.6 mm, 8.68 mm, 8.5 mm, 8.5 mm and 7.6 mm. The inhibition ability of bacterial growth on the disc method is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Whereas in the well method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone was 25.8 mm, 20 mm, 15.6 mm, 12.7 mm and 9.7 mm, respectively. The inhibition ability of bacterial growth in the well method for 100% concentration is categorized as very strong because it has an average inhibition zone diameter of >20 mm, for concentrations of 75%, 50% and 25% it is categorized as strong because it has an average inhibition zone of 10- 20 mm and for a concentration of 12.5% it is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Keywords: Red galangal rhizome, Alpinia purpurata K.Schum, Klebsiella pneumonia  ABSTRAK Rimpang Lengkuas merah merupakan tanaman yang memiliki berbagai khasiat di antaranya sebagai antijamur dan antibakteri. Rimpang Lengkuas merah mengandung golongan senyawa flavonoid, fenol dan terpenoid yang dapat digunakan sebagai bahan dasar obat-obatan moderen. Senyawa flavonoid dan fenol juga diketahui dapat menghambat mikroba. Flavonoid dapat menghambat mikroba yang telah resisten terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak rimpang Lengkuas merah (Alpinia purpurata K.Schum) dan menguji daya hambatnya terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat sputum pada penderita pneumonia resisten antibiotik seftriakson. Tanaman rimpang Lengkuas merah di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji daya hambat antibakteri dilakukan dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak rimpang Lengkuas merah memiliki daya hambat terhadap bakteri Klebsiella pneumoniae. Pada metode cakram untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 9,6 mm, 8,68 mm, 8,5 mm, 8,5 mm dan 7,6 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode cakram di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 25,8 mm, 20 mm, 15,6 mm, 12,7 mm dan 9,7 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode sumuran untuk konsentrasi 100% di kategorikan sangat kuat karena memiliki diameter zona hambat rata-rata >20 mm, untuk konsentrasi 75%, 50% dan 25% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm dan untuk konsentrasi 12,5% di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Kata Kunci:Rimpang Lengkuas merah,  Alpinia purpurata K.Schum, Klebsiella pneumonia

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI Klebsiella pneumonia ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 982
Author(s):  
Novanda S. Puasa ◽  
Fatimawali Fatimawali ◽  
Weny Wiyono
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Average Diameter ◽  
Modern Medicine ◽  
Klebsiella Pneumonia ◽  
Clear Zone ◽  
Activity Test ◽  
Tract Infections ◽  
Antibacterial And Antifungal ◽  
Alpinia Purpurata

ABSTRACTRed galangal rhizome is a plant that has various properties including as an antibacterial and antifungal. Red galangal rhizome in addition to containing essential oils also contains flavonoid, phenol and terpenoid compounds. Red galangal is also used as a basic ingredient of modern medicine. The content of flavonoid compounds which are thought to inhibit the growth of bacteria. This study aims to determine the antibacterial activity of red galangal rhizome extract (Alpinia purpurata K.Schum) against the growth of Klebsiella pneumoniae bacteria of urine isolates in urinary tract infections patients. Red galangal rhizome plants were extracted by maceration method using 96% ethanol solvent. Antibacterial activity test was carried out using the disc and wells method. The results showed that the red galangal rhizome extract has antibacterial activity against the bacteria Klebsiella pneumoniae. In the disc method for concentrations of 80%, 60%, 40%, 20% and 10% the average diameter of the clear zone is 13 mm, 12.3 mm, 10.6 mm, 10.5 mm and 9.8 mm. The ability of antibacterial activity is included in the strong category because it has an inhibition of 10-20 mm. In the method of wells the average diameter of the clear zone is 15.6 mm, 14.6 mm, 13.6 mm, 13 mm and 11.3 mm. The ability of antibacterial activity in the well method of concentrations of 80%, 60%, 40%, 20% and 10% is included in the strong category because the average diameter of inhibition is 10-20 mm.Keywords: Alpinia purpurata K. Schum, Klebsiella pneumoniae, Red Galangal Rhizome ABSTRAKRimpang Lengkuas merah merupakan tanaman yang memiliki berbagai khasiat di antaranya sebagai antibakteri dan antijamur. Rimpang Lengkuas merah selain mengandung minyak atsiri juga mengandung golongan senyawa flavonoid, fenol dan terpenoid. Lengkuas Merah juga digunakan sebagai bahan dasar obat-obatan moderen. Kandungan senyawa flavonoid yang diduga dapat menghambat pertumbuhan bakteri yang telah resisten antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak rimpang Lengkuas merah (Alpinia purpurata K.Schum) terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin pada penderita infeksi saluran kemih. Tanaman rimpang Lengkuas merah di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antibakteri dilakukan dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak rimpang Lengkuas merah memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae. Pada metode cakram untuk konsentrasi 80%, 60%, 40%, 20% dan 10% rata-rata diameter zona beningnya 13 mm, 12,3 mm, 10,6 mm, 10,5 mm dan 9,8 mm. Kemampuan aktivitas antibakteri termasuk dalam kategori kuat karena memiliki daya hambat 10 – 20 mm. Pada metode sumuran rata-rata diameter zona beningnya ialah 15,6 mm, 14,6 mm, 13,6 mm, 13 mm dan 11,3 mm.  Kemampuan aktivitas antibakteri pada metode sumuran konsentrasi 80%, 60%, 40%, 20% dan 10% termasuk dalam kategori kuat karena rata-rata diameter daya hambatnya 10 – 20 mm.Kata kunci : Rimpang Lengkuas merah, Alpinia purpurata K.Schum, Klebsiella pneumoniae

scholarly journals ANTIBACTERIAL ACTIVITY Streptomyces spp. ENDHOPHYTIC TO Staphylococcus aureus AND Eschericia coli

2021 ◽  
Vol 4 (1) ◽  
pp. 19-25
Author(s):  
Siti Nur Azizah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Clear Zone ◽  
Zone Diameter ◽  
Streptomyces Spp ◽  
Agar Plug ◽  
Streptomyces Griseoruber

The objective of this research was to known antibacterial activity of 4 endhophytic Streptomyces namely Streptomyces griseorubiginosus,   Streptomyces vellosus, Streptomyces diastaticus and Streptomyces griseoruber against Staphylococcus aureus and Eschericia coli and measure the biggest antibacterial activity which is indicate by clear zone diameter. Antibacterial activity by using agar plug diffusion method showed that Streptomyces vellosus has capable to inhibits growth of Staphylococcus aureus but not with Eschericia coli and Streptomyces diastaticus has capable to inhibits growth of Eschericia coli but not with Staphylococcus aureus. The clear zone of  Streptomyces vellosus and          Streptomyces diastaticus are 5,18 mm and 7 mm respectively.                Streptomyces griseoruber and Streptomyces griseorubiginosus can’t grow during isolation.  Keyword: Streptomyces vellosus, Streptomyces diastaticus, agar plug diffusion method

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 7 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITIES LEAVES EXTRACTS OF CERBERA MANGHAS AND LEAVES EXTRACTS OF AZADIRACHTA INDICA AGAINST KLEBSIELLA PNEUMONIAE

2018 ◽  
Vol 11 (15) ◽  
pp. 51
Author(s):  
Muhammad Yanis Musdja ◽  
Markhamatul Aeni ◽  
Ira Djajanegara
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Hexane Extract ◽  
Klebsiella Pneumonia ◽  
Methanol Extracts ◽  
Neem Leaves ◽  
Mango Leaves ◽  
Cerbera Manghas

Objective: This study aims to determine the potential of neem (Azadirachta indica, Juss) leaves and sea mango (Cerbera manghas, Linn.) leaves against Klebsiella pneumonia. Methods: Hexane and methanol extracts of neem leaves (A. indica, Juss) and hexane and butanol extracts of sea mango leaves (C. manghas, Linn.) were tested antibacterial against K. pneumonia using paper disc and dilution methods, measured the inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC), as a comparison used ampicillin.Results: The MIC values for the butanol extract and hexane extract of sea mango leaves against K. pneumoniae were 1.124276 and 1.45958 mg/mL, respectively. While the MBC value of both of them had the same value, i.e. 2 mg/ml. The hexane and methanol extracts of neem leaves did not have potentially against the growth of K. pneumonia. Conclusion: Based on the results of this study have been obtained, that extracts butanol and hexane extract of leaves of sea mango (C. manghas, Linn.) have antibacterial activity strong enough against K. pneumonia, where, extracts butanol leaves of sea mango has antibacterial activity that is more powerful than the extract hexane leaf sea mango, while the methanol extract and hexane extracts of neem (A. indica, Juss) leaves have weak activity against K. pneumonia.

scholarly journals PENGARUH PEMBENTUK FILM PVA PADA SEDIAAN MASKER GEL PEEL-OFF EKSTRAK ETANOL BAWANG DAYAK (Eleutherina palmifolia (L.) Merr) TERHADAP AKTIVITAS ANTIBAKTERI Staphylococcus aureus PENYEBAB JERAWAT

2018 ◽  
Vol 15 (2) ◽  
pp. 24
Author(s):  
Husnani Husnani ◽  
Fitri Sri Rizki
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Onion Extract ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Inhibitory Power

ABSTRACTDayak Onion (Eleutherine palmifolia) is one of the plants that can inhibit Staphylococcus aureus bacteria that causes acne. This study aims to determine the effect of polyvinyl alcohol on peel-off gel mask preparations from onion dayak ethanol extract on the antibacterial activity of Staphylococcus aureus that causes acne. Extraction using maceration method with 96% ethanol. The concentration of dayak onion extract was made at 5%, 7.5%, and 10% and the peel-off gel mask formula from dayak onion extract with variations in extract concentration were 5%, 7.5%, and 10% based on Polyvinyl Alcohol (PVA). Antibacterial activity is carried out using the disk disc method. In testing the inhibition of dayak onion extract at a concentration of 5% resulted in a clear zone of 15.5 mm, a concentration of 7.5% and 10% producing the same clear zone diameter of 17.1 mm. The peel-off gel mask in formulation I obtained a clear zone diameter of 8.7 mm, formulation II the diameter of the clear zone was 12.2 mm, and formula III the diameter of the clear zone was 15.3 mm. The results of this study indicate that on dayak onion extract has a greater inhibitory power compared to the dayak onion extract peel-off gel mask preparation. It can be concluded that the PVA in the preparation affects the amount of inhibitory power on the bacteria forming acne, namely Staphylococcus aureus.Keywords: Dayak onion extract, Staphylococcus aureus, disk discs, antibacterial inhibitory test, acne-causing bacteria, peel-off gel mask.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANGLENGKUAS PUTIH (Alpinia galanga L. Willd) TERHADAPBAKTERI Klebsiella pneumoniae ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 975
Author(s):  
Arini Shintia ◽  
Fatimawali Fatimawali ◽  
Jainer P. Siampa
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Alpinia Galanga ◽  
Inhibitory Ability ◽  
Activity Test ◽  
Ethanol Extracts ◽  
Tract Infections

ABSTRACTWhite galangal rhizome is a plant that has properties such as antifungal and antibacterial. White galangal rhizome contains of active compounds, namely flavonoid, phenol and terpenoid compounds which can inhibit microbes. This study aimed to determine the antibacterial activity of ethanol extracts of white galangal rhizome against Klebsiella pneumoniae urine isolate in patients with urinary tract infections. White galangal rhizome plants were extracted using 96% ethanol solvent. Antibacterial activity test was performed using the disc and wells method. The result showed that ethanol extracts of white galangal rhizome has antibacterial activity against the bacteria Klebsiella pneumoniae. In the disc method with concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone formed are 9,8 mm; 9 mm; 7,8 mm; 7,9 mm and 7,7 mm. Inhibition of the disc method is categorized as medium because the diameter of the zone formed is only around an average of 5-10 mm. Whereas in the wells method for concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone are 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm and 2,6 mm. The inhibitory ability at concentrations of 80% and 60% is categorized as strong because it has an average of 10-20 mm, for concentrations of 40% and 20% are categorized as medium, because it has an average inhibition zone diameter of 5-10 mm, and for concentration of 10% is categorized as weak because it has a inhibition diameter <5 mm.Keywords: White Galangal Rhizome (Alpinia galanga L. Willd), Klebsiella pneumonia ABSTRAKRimpang lengkuas putih merupakan tanaman yang memiliki khasiat di antaranya sebagai antifungi dan antibakteri. Rimpang Lengkuas putih mengandung golongan senyawa aktif yaitu golongan senyawa flavonoid, fenol dan terpenoid yang dapat menghambat mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol rimpang Lengkuas putih terhadap bakteri Klebisella pneumoniae isolat urin pada penderita infeksi saluran kemih. Tanaman rimpang Lengkuas putih di ekstraksi dengan menggunakan pelarut etanol 96%. Uji aktivitas antibakteri dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak etanol rimpang Lengkuas putih memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae. Pada metode cakram dengan konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambat yang terbentuk ialah 9,8 mm; 9 mm; 7,8 mm; 7,9 mm dan 7,7 mm. Penghambatan pada metode cakram dikategorikan sedang karena diameter zona yang terbentuk hanya berkisar pada rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambatnya ialah 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm dan 2,6 mm. Kemampuan penghambatan pada konsentrasi 80% dan 60% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm, untuk konsentrasi 40% dan 20% di kategorikan sedang, karena memiliki diameter zona hambat rata-rata 5-10 mm, dan untuk konsentrasi 10% di kategorikan lemah karena memiliki diameter zona hambat <5 mm. Kata Kunci: Rimpang lengkuas putih (Alpinia galanga L. Willd), Klebsiella pneumoniae

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 1 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals UJI DAYA HAMBAT NANOPARTIKEL EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP PERTUMBUHAN BAKTERI Klebsiella pneumoniae ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH RESISTEN ANTIBIOTIK CIPROFLOXACIN

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 870
Author(s):  
Misella R. C. Lasut ◽  
Fatimawali Fatimawali ◽  
Irma Antasionasti
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Klebsiella Pneumonia ◽  
Antibiotic Resistant ◽  
Phenol Compounds ◽  
Extract Concentration ◽  
Treatment Dose ◽  
Tract Infections ◽  
Alpinia Purpurata ◽  
Growth Of Bacteria

ABSTRACTNanoparticles can increase the solubility of compounds, reduce the treatment dose and increase absorption. Flavonoid and phenol compounds in red galangal can inhibit the growth of bacteria that have been resistant to antibiotics. The bioactivity of red galangal compounds is expected to increase when the size of nanoparticles increases antibacterial activity. This study aims to determine the inhibition of the content of Red Galangal nanoparticles (Alpinia purpurata K.Schum) using chitosan on the growth of Klebsiella pneumoniae bacterial urine isolates of UTI antibiotic-resistant Ciprofloxacin. Nanoparticles extracts of red galangal rhizome was made using ionic gelation method, using extract concentration of 0.4%, tested for antibacterial activity using the wells method with a comparison of chitosan 0.4%, acetic acid 5%, red galangal extract 25%. Red galangal rhizome nanoparticles gave antibacterial activity value to the bacteria Klebsiella pneumoniae of 13.5 mm. Therefore, red galangal extracts nanoparticles can inhibit the growth of Klebsiella pneumoniae urine isolate in patients with urinary tract infections with strong inhibitory categories based on Davis and Stout categories.Keywords: Alpinia purpurata K. Schum, Klebsiella pneumonia, Nanoparticles. ABSTRAK Nanopartikel dapat meningkatkan kelarutan senyawa, mengurangi dosis pengobatan dan meningkatkan absorbsi. Senyawa flavonoid dan fenol dalam lengkuas merah mampu menghambat pertumbuhan bakteri yang telah resisten terhadap antibiotik. Sifat bioaktivitas senyawa lengkuas merah diharapkan akan meningkat ketika berukuran nanopartikel untuk meningkatkan aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat kandungan nanopartikel rimpang Lengkuas Merah (Alpinia purpurata K.Schum) menggunakan kitosan terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin penderita ISK yang resisten antibiotik Ciprofloxacin. Nanopartikel ekstrak rimpang lengkuas merah dibuat dengan menggunakan metode gelasi ionik, dengan menggunakan konsentrasi esktrak sebesar 0,4 %, dilakukan pengujian aktivitas antibakteri mengunakan metode sumuran dengan pembanding kitosan 0,4%, asam asetat 5%, ekstrak lengkuas merah 25%. Nanopartikel rimpang lengkuas merah memberikan nilai aktifitas antibakteri terhadap bakteri Klebsiella pneumoniae sebesar 13,5 mm. Oleh karena itu nanopartikel ekstrak rimpang lengkuas merah dapat menghambat pertumbuhan bakteri Klebsiella pneumoniae isolate urin penderita infeksi saluran kemih dengan kategori daya hambat kuat berdasrkan kategori Davis dan Stout.Kata Kunci: Alpinia purpurata K. Schum, Nanopartikel, Klebsiella pneumoniae

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