scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI Klebsiella pneumonia ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 982
Author(s):  
Novanda S. Puasa ◽  
Fatimawali Fatimawali ◽  
Weny Wiyono
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Average Diameter ◽  
Modern Medicine ◽  
Klebsiella Pneumonia ◽  
Clear Zone ◽  
Activity Test ◽  
Tract Infections ◽  
Antibacterial And Antifungal ◽  
Alpinia Purpurata

ABSTRACTRed galangal rhizome is a plant that has various properties including as an antibacterial and antifungal. Red galangal rhizome in addition to containing essential oils also contains flavonoid, phenol and terpenoid compounds. Red galangal is also used as a basic ingredient of modern medicine. The content of flavonoid compounds which are thought to inhibit the growth of bacteria. This study aims to determine the antibacterial activity of red galangal rhizome extract (Alpinia purpurata K.Schum) against the growth of Klebsiella pneumoniae bacteria of urine isolates in urinary tract infections patients. Red galangal rhizome plants were extracted by maceration method using 96% ethanol solvent. Antibacterial activity test was carried out using the disc and wells method. The results showed that the red galangal rhizome extract has antibacterial activity against the bacteria Klebsiella pneumoniae. In the disc method for concentrations of 80%, 60%, 40%, 20% and 10% the average diameter of the clear zone is 13 mm, 12.3 mm, 10.6 mm, 10.5 mm and 9.8 mm. The ability of antibacterial activity is included in the strong category because it has an inhibition of 10-20 mm. In the method of wells the average diameter of the clear zone is 15.6 mm, 14.6 mm, 13.6 mm, 13 mm and 11.3 mm. The ability of antibacterial activity in the well method of concentrations of 80%, 60%, 40%, 20% and 10% is included in the strong category because the average diameter of inhibition is 10-20 mm.Keywords: Alpinia purpurata K. Schum, Klebsiella pneumoniae, Red Galangal Rhizome ABSTRAKRimpang Lengkuas merah merupakan tanaman yang memiliki berbagai khasiat di antaranya sebagai antibakteri dan antijamur. Rimpang Lengkuas merah selain mengandung minyak atsiri juga mengandung golongan senyawa flavonoid, fenol dan terpenoid. Lengkuas Merah juga digunakan sebagai bahan dasar obat-obatan moderen. Kandungan senyawa flavonoid yang diduga dapat menghambat pertumbuhan bakteri yang telah resisten antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak rimpang Lengkuas merah (Alpinia purpurata K.Schum) terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin pada penderita infeksi saluran kemih. Tanaman rimpang Lengkuas merah di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antibakteri dilakukan dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak rimpang Lengkuas merah memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae. Pada metode cakram untuk konsentrasi 80%, 60%, 40%, 20% dan 10% rata-rata diameter zona beningnya 13 mm, 12,3 mm, 10,6 mm, 10,5 mm dan 9,8 mm. Kemampuan aktivitas antibakteri termasuk dalam kategori kuat karena memiliki daya hambat 10 – 20 mm. Pada metode sumuran rata-rata diameter zona beningnya ialah 15,6 mm, 14,6 mm, 13,6 mm, 13 mm dan 11,3 mm.  Kemampuan aktivitas antibakteri pada metode sumuran konsentrasi 80%, 60%, 40%, 20% dan 10% termasuk dalam kategori kuat karena rata-rata diameter daya hambatnya 10 – 20 mm.Kata kunci : Rimpang Lengkuas merah, Alpinia purpurata K.Schum, Klebsiella pneumoniae

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANGLENGKUAS PUTIH (Alpinia galanga L. Willd) TERHADAPBAKTERI Klebsiella pneumoniae ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 975
Author(s):  
Arini Shintia ◽  
Fatimawali Fatimawali ◽  
Jainer P. Siampa
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Alpinia Galanga ◽  
Inhibitory Ability ◽  
Activity Test ◽  
Ethanol Extracts ◽  
Tract Infections

ABSTRACTWhite galangal rhizome is a plant that has properties such as antifungal and antibacterial. White galangal rhizome contains of active compounds, namely flavonoid, phenol and terpenoid compounds which can inhibit microbes. This study aimed to determine the antibacterial activity of ethanol extracts of white galangal rhizome against Klebsiella pneumoniae urine isolate in patients with urinary tract infections. White galangal rhizome plants were extracted using 96% ethanol solvent. Antibacterial activity test was performed using the disc and wells method. The result showed that ethanol extracts of white galangal rhizome has antibacterial activity against the bacteria Klebsiella pneumoniae. In the disc method with concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone formed are 9,8 mm; 9 mm; 7,8 mm; 7,9 mm and 7,7 mm. Inhibition of the disc method is categorized as medium because the diameter of the zone formed is only around an average of 5-10 mm. Whereas in the wells method for concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone are 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm and 2,6 mm. The inhibitory ability at concentrations of 80% and 60% is categorized as strong because it has an average of 10-20 mm, for concentrations of 40% and 20% are categorized as medium, because it has an average inhibition zone diameter of 5-10 mm, and for concentration of 10% is categorized as weak because it has a inhibition diameter <5 mm.Keywords: White Galangal Rhizome (Alpinia galanga L. Willd), Klebsiella pneumonia ABSTRAKRimpang lengkuas putih merupakan tanaman yang memiliki khasiat di antaranya sebagai antifungi dan antibakteri. Rimpang Lengkuas putih mengandung golongan senyawa aktif yaitu golongan senyawa flavonoid, fenol dan terpenoid yang dapat menghambat mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol rimpang Lengkuas putih terhadap bakteri Klebisella pneumoniae isolat urin pada penderita infeksi saluran kemih. Tanaman rimpang Lengkuas putih di ekstraksi dengan menggunakan pelarut etanol 96%. Uji aktivitas antibakteri dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak etanol rimpang Lengkuas putih memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae. Pada metode cakram dengan konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambat yang terbentuk ialah 9,8 mm; 9 mm; 7,8 mm; 7,9 mm dan 7,7 mm. Penghambatan pada metode cakram dikategorikan sedang karena diameter zona yang terbentuk hanya berkisar pada rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambatnya ialah 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm dan 2,6 mm. Kemampuan penghambatan pada konsentrasi 80% dan 60% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm, untuk konsentrasi 40% dan 20% di kategorikan sedang, karena memiliki diameter zona hambat rata-rata 5-10 mm, dan untuk konsentrasi 10% di kategorikan lemah karena memiliki diameter zona hambat <5 mm. Kata Kunci: Rimpang lengkuas putih (Alpinia galanga L. Willd), Klebsiella pneumoniae

scholarly journals UJI DAYA HAMBAT NANOPARTIKEL EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP PERTUMBUHAN BAKTERI Klebsiella pneumoniae ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH RESISTEN ANTIBIOTIK CIPROFLOXACIN

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 870
Author(s):  
Misella R. C. Lasut ◽  
Fatimawali Fatimawali ◽  
Irma Antasionasti
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Klebsiella Pneumonia ◽  
Antibiotic Resistant ◽  
Phenol Compounds ◽  
Extract Concentration ◽  
Treatment Dose ◽  
Tract Infections ◽  
Alpinia Purpurata ◽  
Growth Of Bacteria

ABSTRACTNanoparticles can increase the solubility of compounds, reduce the treatment dose and increase absorption. Flavonoid and phenol compounds in red galangal can inhibit the growth of bacteria that have been resistant to antibiotics. The bioactivity of red galangal compounds is expected to increase when the size of nanoparticles increases antibacterial activity. This study aims to determine the inhibition of the content of Red Galangal nanoparticles (Alpinia purpurata K.Schum) using chitosan on the growth of Klebsiella pneumoniae bacterial urine isolates of UTI antibiotic-resistant Ciprofloxacin. Nanoparticles extracts of red galangal rhizome was made using ionic gelation method, using extract concentration of 0.4%, tested for antibacterial activity using the wells method with a comparison of chitosan 0.4%, acetic acid 5%, red galangal extract 25%. Red galangal rhizome nanoparticles gave antibacterial activity value to the bacteria Klebsiella pneumoniae of 13.5 mm. Therefore, red galangal extracts nanoparticles can inhibit the growth of Klebsiella pneumoniae urine isolate in patients with urinary tract infections with strong inhibitory categories based on Davis and Stout categories.Keywords: Alpinia purpurata K. Schum, Klebsiella pneumonia, Nanoparticles. ABSTRAK Nanopartikel dapat meningkatkan kelarutan senyawa, mengurangi dosis pengobatan dan meningkatkan absorbsi. Senyawa flavonoid dan fenol dalam lengkuas merah mampu menghambat pertumbuhan bakteri yang telah resisten terhadap antibiotik. Sifat bioaktivitas senyawa lengkuas merah diharapkan akan meningkat ketika berukuran nanopartikel untuk meningkatkan aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat kandungan nanopartikel rimpang Lengkuas Merah (Alpinia purpurata K.Schum) menggunakan kitosan terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin penderita ISK yang resisten antibiotik Ciprofloxacin. Nanopartikel ekstrak rimpang lengkuas merah dibuat dengan menggunakan metode gelasi ionik, dengan menggunakan konsentrasi esktrak sebesar 0,4 %, dilakukan pengujian aktivitas antibakteri mengunakan metode sumuran dengan pembanding kitosan 0,4%, asam asetat 5%, ekstrak lengkuas merah 25%. Nanopartikel rimpang lengkuas merah memberikan nilai aktifitas antibakteri terhadap bakteri Klebsiella pneumoniae sebesar 13,5 mm. Oleh karena itu nanopartikel ekstrak rimpang lengkuas merah dapat menghambat pertumbuhan bakteri Klebsiella pneumoniae isolate urin penderita infeksi saluran kemih dengan kategori daya hambat kuat berdasrkan kategori Davis dan Stout.Kata Kunci: Alpinia purpurata K. Schum, Nanopartikel, Klebsiella pneumoniae

scholarly journals UJI DAYA HAMBAT EKSTRAK HEKSANA RIMPANG LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI Klebsiella pneumoniae ISOLAT URIN PADA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 47
Author(s):  
Firdaus Alamri ◽  
Fatimawali Fatimawali ◽  
Imam Jayanto
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Average Diameter ◽  
Diffusion Method ◽  
Polar Extracts ◽  
Tract Infections ◽  
Alpinia Purpurata ◽  
Paper Disc

ABSTRACT Galangal rhizome traditionally used to treat diseases. Red galangal rhizome contains flavonoid compounds, which are thought to be able to inhibit the growth of bacteria. Urinary tract infections are infections that occur due to the proliferation of microorganisms in the urinary tract. This study aims to determine the antibacterial activity of non-polar extracts of red galangal (Alpinia purpurata K.Schum) against the growth of Klebsiella pneumoniae bacterial urine isolates in urinary tract infections. Extraction was carried out by maceration method using n-hexane solvent. The antibacterial activity was testing using the paper disc diffusion method (Kirby and Bauer diffusion). The results showed that the inhibitory activity of extracts with concentrations of 80%, 40%, 20%, and 10% of the average diameter of the clear zone were 11.03 mm, 8.75 mm, 7.1 mm and 6.03 mm. Shows that the inhibition at 80% concentration is strong, while at 40% concentration, 20% is moderate and at 10% concentration is moderate. Based on this study it can be concluded that the non-polar extract of red galangal can inhibit the bacteria Klebsiella pneumoniae urine isolate in urinary tract infections. Keywords: Red Galangal Rhizome, Antibacterial, Inhibition  ABSTRAK Rimpang lengkuas secara tradisional digunakan untuk mengobati penyakit. Rimpang Lengkuas merah mengandung senyawa golongan flavonoid yang diduga mampu menghambat pertumbuhan bakteri. Infeksi saluran kencing merupakan infeksi yang terjadi akibat berkembangbiaknya mikroorganisme di dalam saluran kemih. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak non-polar rimpang lengkuas merah (Alpinia purpurata K.Schum) terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin pada infeksi saluran kemih. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut n-heksan. Pengujian aktivitas antibakteri menggunakan metode difusi dengan cakram kertas (difusi Kirby dan Bauer). Hasil penelitian menunjukkan bahwa daya hambat ekstrak dengan konsentrasi 80%, 40%, 20%, dan 10% rata-rata diameter  zona beningnya 11,03 mm, 8,75 mm, 7,1 mm, dan 6,03 mm. Menunjukkan bahwa daya hambat pada konsentrasi 80% termasuk kuat, sedangkan pada  konsentrasi  40%,20% termasuk sedang dan pada konsentrasi 10% termasuk  sedang. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak non-polar lengkuas merah dapat menghambat bakteri Klebsiella pneumoniae isolat urin pada infeksi saluran kemih. Kata Kunci: Rimpang Lengkuas Merah, Antibakteri, Daya Hambat

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK N-HEKSAN BIJI BUAH LANGSAT (Lansium domesticum Corr) TERHADAP BAKTERI Staphylococus Aureus DAN Klebsiella Pneumoniae

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 748
Author(s):  
Anastasia P.R. Nurhamidin ◽  
Fatimawali Fatimawali ◽  
Irma Antasionasti
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Hexane Extract ◽  
Klebsiella Pneumonia ◽  
Clear Zone ◽  
Zone Diameter ◽  
Activity Inhibition ◽  
Lansium Domesticum

ABSTRACT Langsat fruit seeds (Lansium domesticum Corr) are  part of the plant that is known to be antibacterial. Therefore, exploration of langsat fruit as an antibacterial is needed. The purpose of this study was to determine the antibacterial activity of langsat fruit seeds against Staphylococus aureus and Klebsiella pneumoniae using the disc method and the well method. Langsat fruit seed powder was macerated using the maceration method with n-hexane solvent and tested for antibacterial activity. Inhibition of the n-hexane extract of langsat fruit seeds showed the largest clear zone diameter value given by the lowest concentration of 10% by the disc and sumurun method on the Staphylococus aureus and Klebsiella pneumoniaes bacteria 13.6 mm; 12.6 mm; 13 mm; and 14.6 mm. This shows that the n-hexane extract of langsat fruit seeds has strong antibacterial activity against Staphylococus aureus and Klebsiella pneumonia bacteria both by the disc method and the well method. Keywords: langsat fruit seeds, Klebsiella pneumoniae, disc, pitting method, Staphylococus aureus  ABSTRAKBiji buah langsat (Lansium domesticum Corr) merupakan salah satu bagian tanaman yang telah dikenal sebagai antibakteri. Maka dari itu, eksplorasi buah langsat sebagai antibakteri sangat diperlukan. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri biji buah langsat terhadap bakteri Staphylococus aureus  dan Klebsiella pneumoniae menggunakan metode cakram dan metode sumuran. Serbuk biji buah langsat dimaserasi menggunakan metode maserasi dengan pelarut n-Heksan dan dilakukan uji aktivitas antibakteri. Daya hambat ekstrak n-Heksan biji buah langsat menunjukkan nilai diameter zona bening terbesar diberikan oleh konsentrasi terendah 10% dengan metode cakram (13,6 mm pada bakteri Staphylococus aureus dan 12,6 mm pada bakteri Klebsiella pneumoniae) dan metode sumuran (13 mm pada bakteri Staphylococus aureus dan 14,6 mm pada bakteri Klebsiella pneumoniae). Hal ini menunjukkan bahwa ekstrak n-Heksan biji buah langsat memiliki aktivitas antibakteri yang kuat terhadap bakteri Staphylococus aureus  dan Klebsiella pneumoniae baik dengan metode cakram maupun metode sumuran. Kata Kunci: biji buah langsat,Klebsiella pneumoniae, metode cakram, sumuran, Staphylococus aureus

scholarly journals UJI DAYA HAMBAT EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K.Schum) TERHADAP PERTUMBUHAN BAKTERI Klebsiella pneumoniae ISOLAT SPUTUM PADA PENDERITA PNEUMONIA RESISTEN ANTIBIOTIK SEFTRIAKSON

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Poetry Melinda Abubakar ◽  
Fatimawali Fatimawali ◽  
Paulina Yamlean
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Growth ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Alpinia Purpurata ◽  
Inhibition Ability

ABSTRACT Red galangal rhizome is a plant that has various properties including antifungal and antibacterial. Red galangal rhizomes contain a class of flavonoids, phenols and terpenoids, which can be, used as basic ingredients of modern medicines. Flavonoids and phenols are also known to inhibit microbes. Flavonoids can inhibit microbes that have been resistant to antibiotics. This study aims to determine the antibacterial activity of red galangal rhizome extract (Alpinia purpurata K.Schum) and test its inhibitory effect on the growth of Klebsiella pneumoniae bacteria in sputum isolates in patients with pneumonia resistant ceftriaxone antibiotics. Red galangal rhizome plant was extracted by maceration method using 96% ethanol. Antibacterial inhibitory tests were carried out using the disc and well method. The results showed that the red galangal extract of rhizome had a inhibitory effect on the bacterium Klebsiella pneumoniae. On the disc method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone is 9.6 mm, 8.68 mm, 8.5 mm, 8.5 mm and 7.6 mm. The inhibition ability of bacterial growth on the disc method is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Whereas in the well method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone was 25.8 mm, 20 mm, 15.6 mm, 12.7 mm and 9.7 mm, respectively. The inhibition ability of bacterial growth in the well method for 100% concentration is categorized as very strong because it has an average inhibition zone diameter of >20 mm, for concentrations of 75%, 50% and 25% it is categorized as strong because it has an average inhibition zone of 10- 20 mm and for a concentration of 12.5% it is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Keywords: Red galangal rhizome, Alpinia purpurata K.Schum, Klebsiella pneumonia  ABSTRAK Rimpang Lengkuas merah merupakan tanaman yang memiliki berbagai khasiat di antaranya sebagai antijamur dan antibakteri. Rimpang Lengkuas merah mengandung golongan senyawa flavonoid, fenol dan terpenoid yang dapat digunakan sebagai bahan dasar obat-obatan moderen. Senyawa flavonoid dan fenol juga diketahui dapat menghambat mikroba. Flavonoid dapat menghambat mikroba yang telah resisten terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak rimpang Lengkuas merah (Alpinia purpurata K.Schum) dan menguji daya hambatnya terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat sputum pada penderita pneumonia resisten antibiotik seftriakson. Tanaman rimpang Lengkuas merah di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji daya hambat antibakteri dilakukan dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak rimpang Lengkuas merah memiliki daya hambat terhadap bakteri Klebsiella pneumoniae. Pada metode cakram untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 9,6 mm, 8,68 mm, 8,5 mm, 8,5 mm dan 7,6 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode cakram di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 25,8 mm, 20 mm, 15,6 mm, 12,7 mm dan 9,7 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode sumuran untuk konsentrasi 100% di kategorikan sangat kuat karena memiliki diameter zona hambat rata-rata >20 mm, untuk konsentrasi 75%, 50% dan 25% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm dan untuk konsentrasi 12,5% di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Kata Kunci:Rimpang Lengkuas merah,  Alpinia purpurata K.Schum, Klebsiella pneumonia

scholarly journals ANTIBACTERIAL ACTIVITY OF FRUIT BANANA STONE AND MECHANISM

2018 ◽  
Vol 11 (13) ◽  
pp. 167
Author(s):  
Panal Sitorus ◽  
Dwi Suryanto, Hepni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Clear Zone ◽  
Activity Test ◽  
Cellular Metabolite ◽  
Cell Leakage

Objective: The purpose of this study was to determine the strength of antibacterial activity of the fraction of banana stone and its mechanism.Methods: The antibacterial activity test was performed using the diffusion method by measuring the diameter of the clear zone around the disc paper.Results: The results of antibacterial activity test showed that ethyl acetate fraction was more effective against bacterium Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes compared to n-hexane fraction.Conclusion: The resulting cellular metabolite leak showed that cell leakage in the three bacteria due to ethyl acetate fraction had leaked more protein than nucleic acid, while the leakage of more dissolved K+ ion than Ca2 + ions.

scholarly journals Antibacterial Activity Test of Endophytic Fungus from Mangrove Plant (Rhizophora apiculata L.) and (Bruguiera gymnorrizha (L.) Lamk.) Against Klebsiella pneumoniae ATCC 700603

2017 ◽  
Vol 2 (6) ◽  
pp. 146
Author(s):  
Rossiana Nia ◽  
Miranti Mia ◽  
Kosmita Oktapiana
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Drug Candidate ◽  
Mangrove Plant ◽  
Fungal Extract ◽  
Rhizophora Apiculata ◽  
Activity Test

Pathogenic bacteria Klebsiella pneumoniae is the cause of pneumonia disease in infants and adults. Antibiotics treatment is usually used to treat this disease. However, synthetic antibiotic use could lead to bacterial resistance. Therefore, novel antibiotics from natural resources are required to overcome this problem. The aim of present study is to determine the antibacterial activity of endophytic fungal extracts from mangrove plants Rhizophora apiculata and Bruguiera gymnorrhiza against K. pneumonia ATCC 700 603. Isolation, cultivation, extraction and antibacterial activity test were conducted. Fourteen endophytic fungi were derived from the two mangrove species, where the two fungal isolates: Penicilium sp.2 and Penicilium sp.4 showed the highest antibacterial activity. The minimum inhibitory concentration of K. pneumoniae for both fungal extract is 3.6 mg mL-1, where bactericidal effect was observed. Additionally, antibacterial activity from the extract of Penncillium sp.4 is higher compared to Penicilium sp.2 extract, which is signified by larger average diameter of inhibition zone. However, at concentration more than 3.6 mg mL-1, both extract effectively inhibit the growth of K. pneumoniae. Our study reveals the potential of fungal extract from mangrove plant as prospective drug candidate to overcome the pneumonia disease. Keywords: antibacterial, pneumonia, endophytic fungi, mangrove

scholarly journals Antibacterial Activity of Some Nanoparticles Against Some Pathogenic Bacteria That Isolated From Urinary Tract Infections Patient

2019 ◽  
Vol 9 (04) ◽  
pp. 682-685
Author(s):  
Taha H. Alnasrawi ◽  
Zahraa A. Althabet ◽  
Ghufran S. Salih ◽  
Mohammad J. Al-Jassani
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
High Concentration ◽  
Disk Diffusion Assay ◽  
Tract Infections ◽  
Diffusion Assay ◽  
Economical Alternative

Nanoparticles include multiple metal oxides such as yttrium, copper, nickel, zinc, iron, and silver that have antimicrobial activity. The present work evaluates the antibacterial activity of some nanoparticles (NPs)against various pathogenic bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus). Antimicrobial activity of NPs was accomplished by the mean of disk diffusion assay using dilutions of (200, 100, 50, 25, and 12.5) and the MIC and MBC of each isolate is determined. NPs shows no antibacterial activity against tested bacteria. It is highly recommended using NPs as an economical alternative antibacterial agent, especially in treating ectopic infections but with high concentration since low concentration gives no result without taking the risk of developing resistant bacterial strains as with antibiotics.

scholarly journals Antibacterial Activity of LCB10-0200 against Klebsiella pneumoniae

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1185
Author(s):  
Sang-Hun Oh ◽  
Young-Rok Kim ◽  
Hee-Soo Park ◽  
Kyu-Man Oh ◽  
Young-Lag Cho ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Antibacterial Agent ◽  
In Vitro Susceptibility ◽  
In Vivo Models ◽  
Tract Infections

Klebsiella pneumoniae is one of the important clinical organisms that causes various infectious diseases, including urinary tract infections, necrotizing pneumonia, and surgical wound infections. The increase in the incidence of multidrug-resistance K. pneumoniae is a major problem in public healthcare. Therefore, a novel antibacterial agent is needed to treat this pathogen. Here, we studied the in vitro and in vivo activities of a novel antibiotic LCB10-0200, a siderophore-conjugated cephalosporin, against clinical isolates of K. pneumoniae. In vitro susceptibility study found that LCB10-0200 showed potent antibacterial activity against K. pneumoniae, including the beta-lactamase producing strains. The in vivo efficacy of LCB10-0200 was examined in three different mouse infection models, including systemic, thigh, and urinary tract infections. LCB10-0200 showed more potent in vivo activity than ceftazidime in the three in vivo models against the drug-susceptible and drug-resistant K. pneumoniae strains. Taken together, these results show that LCB10-0200 is a potential antibacterial agent to treat infection caused by K. pneumoniae.

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