scholarly journals Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 299 ◽  
Author(s):  
Jinho Yoon ◽  
Minkyu Shin ◽  
Taek Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Nucleic Acids ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Transition Metal Dichalcogenide ◽  
The Novel ◽  
Functional Nanomaterials ◽  
Complementary Sequences ◽  
Highly Sensitive ◽  
Target Molecules

Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

scholarly journals Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 95 ◽  
Author(s):  
Shuangjiao Xu ◽  
Kehai Zhou ◽  
Dan Fang ◽  
Lei Ma
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Hplc Method ◽  
Fluorescent Detection ◽  
Copper Nanoclusters ◽  
Highly Sensitive ◽  
The Us ◽  
Fluorescence Quenching Mechanism ◽  
First Time

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

scholarly journals Development, characterization and clinical validation of new sensitive immunofluorometric assay for the measurement of serum thyroglobulin

2012 ◽  
Vol 56 (9) ◽  
pp. 658-665 ◽  
Author(s):  
Cláudia C. D. Nakabashi ◽  
Rosa Paula M. Biscolla ◽  
Teresa S. Kasamatsu ◽  
Teresinha T. Tachibana ◽  
Rafaela N. Barcelos ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
High Sensitivity ◽  
Differentiated Thyroid Carcinoma ◽  
Serum Thyroglobulin ◽  
Additional Advantage ◽  
Highly Sensitive ◽  
Long Term Follow Up

OBJECTIVE: In the last decade, data published stressed the role of highly-sensitive thyroglobulin (Tg) assays in the follow-up of differentiated thyroid carcinoma (DTC) patients. The present study describes a new, highly-sensitive Tg assay, compares it with an available commercial assay, and validates it in the follow-up of DTC patients. SUBJECTS AND METHODS: The immunofluorometric high-sensitivity Tg assay is based on monoclonal and polyclonal antibodies produced at our laboratories. It was validated in 100 samples of 87 patients with DTC submitted to total thyroidectomy, 87% of whom also received radioiodine. For correlation, all samples were also tested using a commercial Tg assay (Beckman Access) with functional sensitivity (FS) of 0.1 ng/mL. RESULTS: The new method showed FS of 0.3 ng/mL. The correlation between the two methods was good (r = 0.74; p < 0.0001). The diagnostic sensitivity was 88.9%, and it was increased to 100% when combined with neck US. CONCLUSION: This new, high-sensitivity Tg assay presented a good correlation with Beckman Access assay and with the clinical outcome of the patients. The continuous availability of a validated assay is an additional advantage for long term follow-up of DTC patients. Arq Bras Endocrinol Metab. 2012;56(9):658-65

scholarly journals Sequence-complementarity dependent co-assembly of phosphodiester-linked aromatic donor-acceptor trimers

2021 ◽  
Author(s):  
Nadeema Appukutti ◽  
Alex de Vries ◽  
Prashant Gudeangadi ◽  
Bini Claringbold ◽  
Michelle Garrett ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Nucleic Acids ◽  
Supramolecular Chemistry ◽  
Self Assembly ◽  
Functional Nanomaterials ◽  
Monomer Sequence ◽  
Complementary Sequences ◽  
Sequence Complementarity ◽  
Aromatic Donor ◽  
Donor Acceptor

Development of the interplay between monomer sequence and supramolecular chemistry is critical if chemistry is to recapitulate the properties of proteins and nucleic acids in the synthetic world. We have created sequenced trimers of aromatic donor/acceptor units which participate in charge-transfer interactions, linked by phosphodiesters. Each sequence displays its own characteristic self-assembly, and moreover complementary sequences interact with each other to produce new nanostructures and emergent thermochromism. This finding paves the way towards new functional nanomaterials which make bio-analogous use of sequence to tune structure.

Facile fabrication of highly sensitive and durable cotton fabric-based pressure sensors for motion and pulse monitoring

2021 ◽  
Author(s):  
Yinan Zhao ◽  
Lin Liu ◽  
zhen Li ◽  
Feifei Wang ◽  
Xinxin Chen ◽  
...  
Keyword(s):  
Cotton Fabric ◽  
Pressure Sensors ◽  
High Sensitivity ◽  
Wearable Electronics ◽  
Term Stability ◽  
Long Term Stability ◽  
Highly Sensitive ◽  
Facile Fabrication ◽  
Flexible Pressure Sensors

Design and development of flexible pressure sensors with high sensitivity, long-term stability and simple fabrication processes is a key procedure to fulfill the applications in wearable electronics, e-skin and medical...

scholarly journals Functionalized Carbon Nanotubes with Gold Nanoparticles to Fabricate a Sensor for Hydrogen Peroxide Determination

2012 ◽  
Vol 9 (4) ◽  
pp. 2540-2549 ◽  
Author(s):  
Halimeh Rajabzade ◽  
Parandis Daneshgar ◽  
Elham Tazikeh ◽  
Ramin Zafar Mehrabian
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Ionic Liquid ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Applied Potential ◽  
Carbon Paste ◽  
Experimental Conditions ◽  
Highly Sensitive ◽  
The Matrix

A highly sensitive electrode was prepared based on gold nanoparticles/nanotubes/ionic liquid for measurement of Hydrogen peroxide. Gold nanoparticles of 20–25 nm were synthesized on a nanotube carbon paste electrode by cyclic voltammetry technique while the coverage was controlled by applied potential and time. The gold nanoparticles were modified to form a monolayer on CNT, followed by decoration with ionic liquid for determination of hydrogen peroxide. The experimental conditions, applied potential and pH, for hydrogen peroxide monitoring were optimized, and hydrogen peroxide was determined amperometrically at 0.3 V vs. SCE at pH 7.0. Electrocatalytic effects of gold deposited CNT were observed with respect to unmodified one. The sensitivity obtained was 5 times higher for modified one. The presence of Au particles in the matrix of CNTs provides an environment for the enhanced electrocatalytic activities. The sensor has a high sensitivity, quickly response to H2O2and good stability. The synergistic influence of MWNT, Au particles and IL contributes to the excellent performance for the sensor. The sensor responds to H2O2in the linear range from 0.02 µM to 0.3 mM. The detection limit was down to 0.4 µM when the signal to noise ratio is 3.

Highly sensitive non-enzymatic electrochemical glucose sensor surpassing water oxidation interference

2021 ◽  
Author(s):  
Neha Thakur ◽  
Debaprasad Mandal ◽  
Tharamani Chikka Nagaiah
Keyword(s):  
Water Oxidation ◽  
Glucose Sensor ◽  
High Sensitivity ◽  
The Novel ◽  
Enzymatic Sensor ◽  
Highly Sensitive ◽  
Sensitive Determination

An electrochemical non-enzymatic sensor based on NiVP/Pi material was developed for the selective and sensitive determination of glucose. The novel sensor shows ultra-high sensitivity of 6.04 mA μM-1 cm-2 with...

scholarly journals Визначення чутливості виробничого штаму Bacillus anthracis UA–07 до антибіотиків

2017 ◽  
Vol 19 (73) ◽  
pp. 84-88
Author(s):  
I. Rublenko ◽  
V. Skripnik
Keyword(s):  
Bacillus Anthracis ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Growth Delay ◽  
Genetic Changes ◽  
Stunted Growth ◽  
Highly Sensitive ◽  
And Control ◽  
Zone Growth

Long-term use of antibiotics leads to antibiotic resistance in pathogens. In addition, there is a possibility of permanent genetic changes in strains due to environmental influences. Therefore there is a need for periodic study vaccine strains for sensitivity to antibiotics in order to study and control of their passport characteristics. The aim of our research was necessary to determine the sensitivity of the strain of Bacillus anthracis UA-07 to antibiotics. The results of studies of sensitivity of strain Bacillus anthracis UA–07 to 50 antibiotics were determined by the size of the diameter of the zones of growth retardation. Zone growth delay of 15 mm was considered a sign of a weak antibiotic sensitivity, zone 15–24 mm – a sign of sensitivity and area of more than 24 mm - a sign of the high sensitivity of the strain to this antibiotic. Lack of stunted growth areas points to the insensitivity of the strain to this antibiotic. The research results show that the strain of Bacillus anthracis UA–07 is highly sensitive to ofloxacin (Of5), ampicillin / sulbactam (A/S10/10), linezolid (LZ30), amoxicillin (AMX30), norfloxacin (NX10), cefalotin (CEP30), tetracycline (TE30). Not affect the development of the studied microorganism antibiotics, cefepime (SPM30), nystatin (NS100U) tsefuroksyn (CXM30) tsefeksym (CFM5), sulfadiazine (SZ300), metronidazole (MT4), cefazolin (Cz30).

scholarly journals Enhancement of amperometric response of glucose biosensor by electrodeposition of silver nanoparticles onto chitosan-modified electrode

2017 ◽  
Author(s):  
Hossein Zare ◽  
Ghasem Najafpour ◽  
Mohsen Jahanshahi ◽  
Mostafa Rahimnejad ◽  
Mohsen Rezvani
Keyword(s):  
Silver Nanoparticles ◽  
Modified Electrode ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Single Pulse ◽  
Glucose Biosensor ◽  
Amperometric Response ◽  
Highly Sensitive ◽  
Menten Constant ◽  
Electrodeposition Of Silver

A highly sensitive biosensor based on silver nanoparticles (AgNPs) was fabricated for glucose detection in aqueous phase. Firstly, a platinum (Pt) electrode was modified with the mixture of glucose oxidase and chitosan. AgNPs were electrodeposited into the modified electrode by single pulse potentiostatic method at –0.4 V. The electrochemical performance of the modified electrode was evaluated by cyclic voltammetry and amperometry. The fabricated biosensor had a high sensitivity of 58.6 μA mM−1 cm−2 and detection limit of 4.4 μM glucose at a signal to noise ratio of 3. In addition, the biosensor showed a short response time less than 5 s and a wide linear range of 0.05-11.5 mM. The apparent Michaelis–Menten constant (KM) was found to be 9.14 mM. In addition, thermal stability and anti-interference ability of the biosensor were investigated. The results demonstrated that AgNPs enhanced the analytical performance of the biosensor.

scholarly journals Remote Temperature Sensor Based on Tamm Resonance

2021 ◽  
Author(s):  
Zaky A. Zaky ◽  
Ashour M. Ahmed ◽  
Arafa Aly
Keyword(s):  
Photonic Crystal ◽  
Temperature Sensor ◽  
Signal To Noise Ratio ◽  
Incident Angle ◽  
High Sensitivity ◽  
Incident Radiation ◽  
High Signal ◽  
Crystal Unit Cell ◽  
Silicon Photonic ◽  
Highly Sensitive

Abstract A highly-sensitive remote temperature sensor based on Tamm resonance is proposed using a one-dimensional photonic crystal. The proposed structure is prism/Ag/Toluene/SiO2 /(PSi1/PSi2)N/Si. The transfer matrix method is used to discuss the interaction between the structure and the S-polarization of the incident radiation waves. We optimized the structure by studying the effect of the incident angle, the thickness of the first and second layers of the photonic crystal unit cell, the porosity of them, and the thickness of the toluene layer. High sensitivity, high signal-to-noise ratio, and very low resolution are achieved due to the coupling between the porous silicon photonic crystal properties and Tamm resonance that makes it very distinguished compared to previous works.

Sign in / Sign up

Export Citation Format

Share Document