scholarly journals Визначення чутливості виробничого штаму Bacillus anthracis UA–07 до антибіотиків

2017 ◽  
Vol 19 (73) ◽  
pp. 84-88
Author(s):  
I. Rublenko ◽  
V. Skripnik
Keyword(s):  
Bacillus Anthracis ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Growth Delay ◽  
Genetic Changes ◽  
Stunted Growth ◽  
Highly Sensitive ◽  
And Control ◽  
Zone Growth

Long-term use of antibiotics leads to antibiotic resistance in pathogens. In addition, there is a possibility of permanent genetic changes in strains due to environmental influences. Therefore there is a need for periodic study vaccine strains for sensitivity to antibiotics in order to study and control of their passport characteristics. The aim of our research was necessary to determine the sensitivity of the strain of Bacillus anthracis UA-07 to antibiotics. The results of studies of sensitivity of strain Bacillus anthracis UA–07 to 50 antibiotics were determined by the size of the diameter of the zones of growth retardation. Zone growth delay of 15 mm was considered a sign of a weak antibiotic sensitivity, zone 15–24 mm – a sign of sensitivity and area of more than 24 mm - a sign of the high sensitivity of the strain to this antibiotic. Lack of stunted growth areas points to the insensitivity of the strain to this antibiotic. The research results show that the strain of Bacillus anthracis UA–07 is highly sensitive to ofloxacin (Of5), ampicillin / sulbactam (A/S10/10), linezolid (LZ30), amoxicillin (AMX30), norfloxacin (NX10), cefalotin (CEP30), tetracycline (TE30). Not affect the development of the studied microorganism antibiotics, cefepime (SPM30), nystatin (NS100U) tsefuroksyn (CXM30) tsefeksym (CFM5), sulfadiazine (SZ300), metronidazole (MT4), cefazolin (Cz30).

scholarly journals Chronic Alcohol Use Induces Molecular Genetic Changes in the Dorsomedial Thalamus of People with Alcohol-Related Disorders

2021 ◽  
Vol 11 (4) ◽  
pp. 435
Author(s):  
Andreas-Christian Hade ◽  
Mari-Anne Philips ◽  
Ene Reimann ◽  
Toomas Jagomäe ◽  
Kattri-Liis Eskla ◽  
...  
Keyword(s):  
Alcohol Use ◽  
Molecular Genetic ◽  
Brain Structures ◽  
Thalamic Nuclei ◽  
Genetic Changes ◽  
Molecular Changes ◽  
Gabaergic Neurotransmission ◽  
And Control ◽  
Upregulated Genes

The Mediodorsal (MD) thalamus that represents a fundamental subcortical relay has been underrepresented in the studies focusing on the molecular changes in the brains of subjects with alcohol use disorder (AUD). In the current study, MD thalamic regions from AUD subjects and controls were analyzed with Affymetrix Clariom S human microarray. Long-term alcohol use induced a significant (FDR ≤ 0.05) upregulation of 2802 transcripts and downregulation of 1893 genes in the MD thalamus of AUD subjects. A significant upregulation of GRIN1 (glutamate receptor NMDA type 1) and FTO (alpha-ketoglutarate dependent dioxygenase) was confirmed in western blot analysis. Immunohistochemical staining revealed similar heterogenous distribution of GRIN1 in the thalamic nuclei of both AUD and control subjects. The most prevalent functional categories of upregulated genes were related to glutamatergic and GABAergic neurotransmission, cellular metabolism, and neurodevelopment. The prevalent gene cluster among down-regulated genes was immune system mediators. Forty-two differentially expressed genes, including FTO, ADH1B, DRD2, CADM2, TCF4, GCKR, DPP6, MAPT and CHRH1, have been shown to have strong associations (FDR p < 10−8) with AUD or/and alcohol use phenotypes in recent GWA studies. Despite a small number of subjects, we were able to detect robust molecular changes in the mediodorsal thalamus caused by alcohol emphasizing the importance of deeper brain structures such as diencephalon, in the development of AUD-related dysregulation of neurocircuitry.

scholarly journals Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 299 ◽  
Author(s):  
Jinho Yoon ◽  
Minkyu Shin ◽  
Taek Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Nucleic Acids ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Transition Metal Dichalcogenide ◽  
The Novel ◽  
Functional Nanomaterials ◽  
Complementary Sequences ◽  
Highly Sensitive ◽  
Target Molecules

Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

scholarly journals Development, characterization and clinical validation of new sensitive immunofluorometric assay for the measurement of serum thyroglobulin

2012 ◽  
Vol 56 (9) ◽  
pp. 658-665 ◽  
Author(s):  
Cláudia C. D. Nakabashi ◽  
Rosa Paula M. Biscolla ◽  
Teresa S. Kasamatsu ◽  
Teresinha T. Tachibana ◽  
Rafaela N. Barcelos ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
High Sensitivity ◽  
Differentiated Thyroid Carcinoma ◽  
Serum Thyroglobulin ◽  
Additional Advantage ◽  
Highly Sensitive ◽  
Long Term Follow Up

OBJECTIVE: In the last decade, data published stressed the role of highly-sensitive thyroglobulin (Tg) assays in the follow-up of differentiated thyroid carcinoma (DTC) patients. The present study describes a new, highly-sensitive Tg assay, compares it with an available commercial assay, and validates it in the follow-up of DTC patients. SUBJECTS AND METHODS: The immunofluorometric high-sensitivity Tg assay is based on monoclonal and polyclonal antibodies produced at our laboratories. It was validated in 100 samples of 87 patients with DTC submitted to total thyroidectomy, 87% of whom also received radioiodine. For correlation, all samples were also tested using a commercial Tg assay (Beckman Access) with functional sensitivity (FS) of 0.1 ng/mL. RESULTS: The new method showed FS of 0.3 ng/mL. The correlation between the two methods was good (r = 0.74; p < 0.0001). The diagnostic sensitivity was 88.9%, and it was increased to 100% when combined with neck US. CONCLUSION: This new, high-sensitivity Tg assay presented a good correlation with Beckman Access assay and with the clinical outcome of the patients. The continuous availability of a validated assay is an additional advantage for long term follow-up of DTC patients. Arq Bras Endocrinol Metab. 2012;56(9):658-65

scholarly journals High sensitivity for tuberculosis in a national integrated surveillance system in Finland

2005 ◽  
Vol 10 (6) ◽  
pp. 3-4 ◽  
Author(s):  
M Kokki ◽  
P Holmström ◽  
P Ruutu
Keyword(s):  
Infectious Disease ◽  
Surveillance System ◽  
Disease Surveillance ◽  
High Sensitivity ◽  
Infectious Disease Surveillance ◽  
Notification System ◽  
Disease Surveillance System ◽  
Highly Sensitive ◽  
And Control ◽  
Culture Positive

Little is known about the sensitivity of surveillance for tuberculosis after integration of formerly dedicated tuberculosis surveillance and control into the general health care system, an integration which took place in Finland in 1987. We compared routine laboratory notifications to the National Infectious Disease Register (NIDR) for Mycobacterium tuberculosis from January 1, 1995, to December 31, 1996, with data collected independently from all laboratories offering M. tuberculosis culture, and with data from patient records. 1059 culture-positive cases were found. The overall sensitivity of the NIDR was 93 % (984/1059). The positive predictive value of a culture-positive case in the NIDR to be a true culture-confirmed case was 99%. For the culture-confirmed cases in the NIDR, one or more physician notification forms had been submitted for 89%. A highly sensitive notification system for culture-positive tuberculosis can be achieved in an integrated national infectious disease surveillance system based on laboratory notification.

scholarly journals Differences in Bacteriological and Antibiotic Sensitivity Patterns in UTI among Hospitalized Diabetic and Nondiabetic Patients

1970 ◽  
Vol 8 (1) ◽  
pp. 10-13
Author(s):  
Mohammed Kamrul Hasan ◽  
Khwaja Nazimuddin ◽  
AKM Shaheen Ahmed ◽  
Rene Suzan Claude Sarker ◽  
Muzammel Haque ◽  
...  
Keyword(s):  
Internal Medicine ◽  
Diabetes Mellitus ◽  
Retrospective Study ◽  
Urine Culture ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Generation Cephalosporin ◽  
Third Generation ◽  
Highly Sensitive

Aim of this study was to determine bacteriological pattern and there antibiotic sensitivity in UTI. The retrospective study carried out in department of internal medicine of BIRDEM on 300 consecutive patients with or without Diabetes Mellitus and whose urine culture shows growth of >105 organism/ml of urine, during the period from January 2006 to July 2007.A total of 300 patients were included in the study. The highest number of isolates was E.coli (62%,184) followed by Klebsiella (17%,50),Pseudomonas(6%,19), Enterococcus(8%, 23), Staphylococcus (3%, 10), Proteus (1%, 4) respectively in patient with DM(N-261) or without DM(N-39).All of these isolates were highly sensitive to Imipenem(96%) while some were high to moderate sensitive to other antibiotics. E.coli isolates showed high sensitivity to Aminoglycosides (72%, 133), Nitrofurantoin (70%, 129), and third generation Cephalosporin (61%, 112). Klebsiella was observed highly sensitive to third generation Cephalosporin (29%, 58) AND Nitrofurantoin (30%, 60).   DOI = 10.3329/jom.v8i1.1373 J MEDICINE 2007; 8 : 10-13

Facile fabrication of highly sensitive and durable cotton fabric-based pressure sensors for motion and pulse monitoring

2021 ◽  
Author(s):  
Yinan Zhao ◽  
Lin Liu ◽  
zhen Li ◽  
Feifei Wang ◽  
Xinxin Chen ◽  
...  
Keyword(s):  
Cotton Fabric ◽  
Pressure Sensors ◽  
High Sensitivity ◽  
Wearable Electronics ◽  
Term Stability ◽  
Long Term Stability ◽  
Highly Sensitive ◽  
Facile Fabrication ◽  
Flexible Pressure Sensors

Design and development of flexible pressure sensors with high sensitivity, long-term stability and simple fabrication processes is a key procedure to fulfill the applications in wearable electronics, e-skin and medical...

scholarly journals ДІАГНОСТИКА ТА ЗАХОДИ БОРОТЬБИ З ПАСТЕРЕЛЬОЗОМ ГУСЕЙ В ПРИВАТНОМУ ЗООПАРКУ ПОЛТАВСЬКОЇ ОБЛАСТІ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 152-157
Author(s):  
O.O. Peredera ◽  
I.V. Lavrinenko ◽  
I.A. Zhernosik
Keyword(s):  
High Efficiency ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Petri Dish ◽  
Growth Zone ◽  
Diffusion Method ◽  
Growth Delay ◽  
Stunted Growth ◽  
Therapeutic Measures ◽  
Moderately Resistant

The results of the study geese pasteurellosis outbreaks in private zoo in the Poltava region. Diagnosis for pasteurellosis set comprehensively, taking into account epizootological features, clinical signs and pathological changes.The diagnosis was carried out comprehensively, taking into account the characteristics of epizootic, clinical signs and postmortem autopsy results. In determining the diagnosis were the results of microscopic and bacteriological research. To study the morphology of microorganisms strokes, the selected cultures were stained with alcoholic–aqueous methylene blue. We studied the sensitivity of selected microorganisms to certain antibiotics. After 24 hours of incubation in nutrient medium grew rounded, smooth, «wax» colonies of microorganisms.In smears of colonies that grew were found to IPA Pasteurella – typical bipolar coli. After obtaining a pure culture was carried determine the sensitivity of the selected pathogen to various antibacterial agents. For this we used disco–diffusion method. After the incubation, the Petri dish looked and measured in millimeters zone delay line growth of microorganisms.According to the results obtained, isolated Pasteurella culture revealed high sensitivity to tilozyn. This antibacterial agent caused stunted growth zone microflora 14–21 mm; High sensitivity also highlighted the culture was determined to enrofloxacin (zone of stunted growth – 16–24 mm) and gentamicin (zone of stunted growth – 18–24 mm) Moderately resistant culture was to kanamycin and chloramphenicol (zone growth retardation – 8–14 mm in accordance). To the rest of the drugs was highlighted microflora resistant, zone microflora growth delay ranged from 0 to 4 mm.The drug Enroksil 10% solution, based on enrofloxacin used orally in drinking water at the rate of 5 ml per 10 liters of water for three days. During treatment birds received only water containing the drug. The high sensitivity of microflora selected a number of antibacterial drugs and high efficiency of therapeutic measures can be explained by timely treatment zoo owner for help with diagnosis.

scholarly journals DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2017
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Lujian Zhou ◽  
Jingjing Zhang ◽  
Jie Du
Keyword(s):  
Signal Amplification ◽  
Tumor Suppressor Genes ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
And Control ◽  
High Sensitivity Detection

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

scholarly journals (52) Postharvest Alleviation of Chilling Injury and Diseases of Cantaloupe Melons

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1088B-1088
Author(s):  
Christian Krarup ◽  
Oscar Núñez
Keyword(s):  
Chilling Injury ◽  
Disease Response ◽  
Long Term Storage ◽  
Heat Treated ◽  
Highly Sensitive ◽  
Postharvest Life ◽  
And Control ◽  
Term Storage ◽  
Main Factor

Chilling injury of cantaloupe melons (Cucumismelo, Cantalupensis group), although variable among cultivars, precludes the use of temperatures close to 0 °C for long term storage or transport. Diseases, either associated with chilling or independent from it, are usually the main factor terminating postharvest life of these melons. `Colima', a Western Shipper cultivar highly sensitive to chilling, was used to evaluate chilling and disease response to the following treatments: immersion in water at 60 °C for 1 minute (with or without 150 ppm chlorine), individual packaging (PVC, whole or perforated), exposure to air at 38 °C for 12 hours (with or without individual packaging), and control. After storage for 18 days at 0 °C and a simulated retail period of 3 days at 20 °C, there were significant differences among treatments: chilling was alleviated in heat-treated and especially in plastic-wrapped fruit, and the presence of diseases caused by pathogens such as Alternaria, Cladosporium, Fusarium, and Rhizopus was markedly reduced by heat treatments. Overall, visual quality was high and superior in fruit immersed in water at 60 °C with 150 ppm chlorine due to almost complete supression of diseases. Project financed by FONDECYT 1020882.

scholarly journals Mismatch between bird species sensitivity and the protection of intact habitats across the Americas

2021 ◽  
Author(s):  
Victor Cazalis ◽  
Megan D. Barnes ◽  
Alison Johnston ◽  
James E.M. Watson ◽  
Cagan H. Şekercioğlu ◽  
...  
Keyword(s):  
Protected Areas ◽  
Species Conservation ◽  
Bird Species ◽  
High Sensitivity ◽  
Species Sensitivity ◽  
Human Pressure ◽  
International Policy ◽  
Highly Sensitive ◽  
Low Coverage

AbstractProtected areas, the most prevalent international policy mechanism for biodiversity conservation, are highly heterogeneous in their effectiveness at buffering ecosystems and species’ habitats from human pressure. Protected areas with intense human pressure cannot protect species that are highly sensitive to human activities. Here, we use 60 million bird observations from the eBird citizen science platform to estimate the sensitivity to human pressure of each bird species breeding in the Americas (Nearctic and Neotropical regions). We find that high-sensitivity species, while found in all ecoregions, are concentrated in the tropical biomes. Ecoregions with large proportions of high-sensitivity species do not have more intact protected habitat, resulting in a low coverage of intact protected habitat for many high-sensitivity species. What is more, 139 high-sensitivity species have little or no intact protected habitat within their distributions while being threatened with extinction. Finally, we show that protected area intactness is decreasing faster in ecoregions with many high-sensitivity species. Our results highlight a major mismatch between species conservation needs and the coverage of intact protected habitats, and will likely hamper the long-term effectiveness of protected areas at retaining species. We highlight ecoregions where the protection and management of intact habitats, complemented by the restoration of degraded ones, is urgently needed to avoid extinctions.

Sign in / Sign up

Export Citation Format

Share Document