scholarly journals Enhancement of amperometric response of glucose biosensor by electrodeposition of silver nanoparticles onto chitosan-modified electrode

2017 ◽  
Author(s):  
Hossein Zare ◽  
Ghasem Najafpour ◽  
Mohsen Jahanshahi ◽  
Mostafa Rahimnejad ◽  
Mohsen Rezvani
Keyword(s):  
Silver Nanoparticles ◽  
Modified Electrode ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Single Pulse ◽  
Glucose Biosensor ◽  
Amperometric Response ◽  
Highly Sensitive ◽  
Menten Constant ◽  
Electrodeposition Of Silver

A highly sensitive biosensor based on silver nanoparticles (AgNPs) was fabricated for glucose detection in aqueous phase. Firstly, a platinum (Pt) electrode was modified with the mixture of glucose oxidase and chitosan. AgNPs were electrodeposited into the modified electrode by single pulse potentiostatic method at –0.4 V. The electrochemical performance of the modified electrode was evaluated by cyclic voltammetry and amperometry. The fabricated biosensor had a high sensitivity of 58.6 μA mM−1 cm−2 and detection limit of 4.4 μM glucose at a signal to noise ratio of 3. In addition, the biosensor showed a short response time less than 5 s and a wide linear range of 0.05-11.5 mM. The apparent Michaelis–Menten constant (KM) was found to be 9.14 mM. In addition, thermal stability and anti-interference ability of the biosensor were investigated. The results demonstrated that AgNPs enhanced the analytical performance of the biosensor.

The Electrochemical Biosensor Based on the Immobilization of Horseradish Peroxidase on TiO2 Nanoneedles Modified Electrodes

2011 ◽  
Vol 239-242 ◽  
pp. 2499-2502
Author(s):  
Nan Nan Wei ◽  
Jiang Yan Du
Keyword(s):  
Horseradish Peroxidase ◽  
Modified Electrode ◽  
Electrochemical Biosensor ◽  
Signal To Noise Ratio ◽  
Direct Electron Transfer ◽  
Modified Electrodes ◽  
Signal To Noise ◽  
Formal Potential ◽  
Amperometric Response ◽  
Transfer Study

A new electrochemical biosensor based on the immobilization of horseradish peroxidase(HRP) on TiO2 nanoneedles modified electrodes has been fabricated. The direct electrochemical response of HRP immobilized on the modified electrode was dramatically enhanced. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of -0.379 V (vs. SCE). The HRP/TiO2 nanoneedles modified electrode exhibited a remarkable electrocatalytic activity toward the oxidation of H2O2. The amperometric response to H2O2 showed a linear range of 4–700μmol/L, with the calculated detection limit of 0.78 μmol/L at a signal-to-noise ratio of three. The modified electrode displayed an acceptable reproducibility and good stability. The new HRP/TiO2 nanoneedles matrix is expected to have wide applications for enzymes and proteins immobilization and direct electron transfer study and opened a way for low conductivity electrode biosensors.

scholarly journals Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 95 ◽  
Author(s):  
Shuangjiao Xu ◽  
Kehai Zhou ◽  
Dan Fang ◽  
Lei Ma
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Hplc Method ◽  
Fluorescent Detection ◽  
Copper Nanoclusters ◽  
Highly Sensitive ◽  
The Us ◽  
Fluorescence Quenching Mechanism ◽  
First Time

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

scholarly journals In-Situ Grown Silver Nanoparticles on Nonwoven Fabrics Based on Mussel-Inspired Polydopamine for Highly Sensitive SERS Carbaryl Pesticides Detection

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 384 ◽  
Author(s):  
Zhiliang Zhang ◽  
Tiantian Si ◽  
Jun Liu ◽  
Guowei Zhou
Keyword(s):  
Silver Nanoparticles ◽  
Collection Efficiency ◽  
High Sensitivity ◽  
Sers Substrate ◽  
Analytical Sensitivity ◽  
Sers Substrates ◽  
Rapid Sampling ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

The rapid sampling and efficient collection of target molecules from a real-world surface is fairly crucial for surface-enhanced Raman scattering (SERS) to detect trace pesticide residues in the environment and in agriculture fields. In this work, a versatile approach was exploited to fabricate a flexible SERS substrate for highly sensitive detection of carbaryl pesticides, using in-situ grown silver nanoparticles (AgNPs)on non-woven (NW) fabric surfaces based on mussel-inspired polydopamine (PDA) molecules. The obtained NW@PDA@AgNPs fabrics showed extremely sensitive and reproducible SERS signals toward crystal violet (CV) molecules, and the detection limit was as low as 1.0 × 10−12 M. More importantly, these NW@PDA@AgNPs fabrics could be directly utilized as flexible SERS substrates for the rapid extraction and detection of trace carbaryl pesticides from various fruit surfaces through a simple swabbing approach. It was identified that the detection limits of carbaryl residues from apple, orange, and banana surfaces were approximately decreased to 4.02 × 10−12, 6.04 × 10−12, and 5.03 × 10−12 g, respectively, demonstrating high sensitivity and superior reliability. These flexible substrates could not only drastically increase the collection efficiency from multifarious irregular-shaped matrices, but also greatly enhance analytical sensitivity and reliability for carbaryl pesticides. The fabricated flexible and multifunctional SERS substrates would have great potential to trace pesticide residue detection in the environment and bioscience fields.

scholarly journals Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 299 ◽  
Author(s):  
Jinho Yoon ◽  
Minkyu Shin ◽  
Taek Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Nucleic Acids ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Transition Metal Dichalcogenide ◽  
The Novel ◽  
Functional Nanomaterials ◽  
Complementary Sequences ◽  
Highly Sensitive ◽  
Target Molecules

Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

scholarly journals Novel amperometric glucose biosensor based on MXene nanocomposite

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
R. B. Rakhi ◽  
Pranati Nayak ◽  
Chuan Xia ◽  
Husam N. Alshareef
Keyword(s):  
Au Nanoparticles ◽  
Synergistic Effects ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Carbon Electrode ◽  
Potential Candidate ◽  
Amperometric Response ◽  
Excellent Stability ◽  
Electrochemical Transducer

Abstract A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

scholarly journals Gold Nanoparticle-Polyaniline Composite Films for Glucose Sensing

2008 ◽  
Vol 8 (6) ◽  
pp. 3158-3163 ◽  
Author(s):  
Pratibha Pandey ◽  
S. P. Singh ◽  
Sunil K. Arya ◽  
Anju Sharma ◽  
Monika Datta ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Indium Tin Oxide ◽  
Composite Films ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Covalent Immobilization ◽  
Amperometric Response ◽  
Electrochemically Deposited ◽  
Response Current ◽  
Menten Constant

Gold nanoparticles (AuNPs) have been self-assembled onto electrochemically deposited polyaniline (PANI) films on indium-tin-oxide (ITO) coated glass plates to fabricate glucose biosensor. The covalent immobilization of glucose oxidase (GOx) in the near vicinity of gold nanoparticles has been obtained using N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS), chemistry between amino groups of PANI and COOH groups of GOx. These AuNPs-PANI/ITO and GOx/AuNPs-PANI/ITO composite films have been characterized using Fourier transform infra red (FTIR) and cyclic voltammetry (CV) techniques, respectively. The fast electron transfer from the modified PANI surface to electrode is indicated by the observed increase in amperometric response current of these GOx/AuNPs-PANI/ITO bioelectrodes. These GOx/AuNPs-PANI/ITO bioelectrodes exhibit response time of 10 s, linearity from 50 to 300 mg/dl and show value of apparent Michaelis-Menten constant (Kmapp) of 2.2 mM.

AgAuPt nanocages for highly sensitive detection of hydrogen peroxide

RSC Advances ◽  
2015 ◽  
Vol 5 (11) ◽  
pp. 7854-7859 ◽  
Author(s):  
Yang Peng ◽  
Ziren Yan ◽  
Ying Wu ◽  
Junwei Di
Keyword(s):  
Hydrogen Peroxide ◽  
Modified Electrode ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

AgAuPt hybrid nanocages modified electrode showed high sensitivity for the detection of hydrogen peroxide.

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