scholarly journals Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

2018 ◽  
Vol 20 (2) ◽  
pp. 432-438 ◽  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Sole Source ◽  
Bacterial Consortium ◽  
Soil Samples ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Biochemical Tests ◽  
Polluted Sites ◽  
Achromobacter Sp

In this study, actual soil samples, taken from Kharg Island, Iran, with high levels of total petroleum hydrocarbons (TPHs) were bioremediated by a bacterial consortium. The strains were: Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., Brevibacillus sp. and Staphylococcus sp., which were isolated from two highly polluted sites: Kharg Island and Kermanshah Oil Refinery. The bacteria were identified and confirmed by biochemical tests and the PCR assay. In order to isolate the bacteria, the enrichment method, in which crude oil (2%) was utilized as the sole source of carbon and energy, was used. The results showed that the adapted bacterial consortium could degrade nearly 60% of the pollution. Also, it was found that after 100 days of bioremediation only Brevibacillus sp. could survive in the environment.

scholarly journals Potential of Biosurfactants’ Production on Degrading Heavy Oil by Bacterial Consortia Obtained from Tsunami-Induced Oil-Spilled Beach Areas in Miyagi, Japan

2020 ◽  
Vol 8 (8) ◽  
pp. 577
Author(s):  
Sandia Primeia ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Heavy Oil ◽  
Petroleum Hydrocarbons ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Treatment Plant ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Potential Candidate ◽  
Bacterial Consortia ◽  
Achromobacter Sp ◽  
Degradation Ability

Bioremediation is one of the promising environment-friendly approaches to eliminate oil contamination. However, heavy oil is known to degrade slowly due to its hydrophobicity. Therefore, microorganisms capable of producing biosurfactants are gaining substantial interest because of their potential to alter hydrocarbon properties and thereby speed up the degradation process. In this study, six bacterial consortia were obtained from the oil-spilled beach areas in Miyagi, Japan, and all of which exhibited high potential in degrading heavy oil measured by gas chromatography with flame ionization detector (GC-FID). The polymerase chain reaction—denaturing gradient gel electrophoresis (PCR-DGGE) and next-generation sequencing (NGS) revealed that the diverse microbial community in each consortium changed with subculture and became stable with a few effective microorganisms after 15 generations. The total petroleum hydrocarbons (TPH) degradation ability of the consortia obtained from a former gas station (C1: 81%) and oil refinery company (C6: 79%) was higher than that of the consortia obtained from wastewater treatment plant (WWTP) (C3: 67%, and C5: 73%), indicating that bacteria present in C1 and C6 were historically exposed to petroleum hydrocarbons. Moreover, it was intriguing that the consortium C4, also obtained from WWTP, exhibited high TPH degradation ability (77%). The NGS results revealed that two bacteria, Achromobacter sp. and Ochrobactrum sp., occupied more than 99% of the consortium C4, while no Pseudomonas sp. was found in C4, though this bacterium was observed in other consortia and is also known to be a potential candidate for TPH degradation as reported by previous studies. In addition, the consortium C4 showed high biosurfactant-producing ability among the studied consortia. To date, no study has reported the TPH degradation by the combination of Achromobacter sp. and Ochrobactrum sp.; therefore, the consortium C4 provided an excellent opportunity to study the interaction of and biosurfactant production by these two bacteria during TPH degradation.

Mobility of total petroleum hydrocarbons in Shambat soil in Sudan

2014 ◽  
Vol 11 (2) ◽  
pp. 134-143
Author(s):  
Nuhad M. Ali ◽  
Sarra A.M. Saad ◽  
Elamin A. Elamin
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Soil Column ◽  
Total Petroleum Hydrocarbons ◽  
Downward Mobility ◽  
Content Type ◽  
Oil Concentration ◽  
Polluted Sites ◽  
Different Depths ◽  
Oil Company

Purpose – A laboratory experiment was conducted in the soil laboratory in the Department of Environment and Environmental Pollution, Environment and Natural Resources Research Institute, National Centre for Research. The purpose of this paper is to study the movement of crude oil through soil column. Design/methodology/approach – Polyvinyl chloride columns were filled with Shambat soil, amended with three concentrations of light crude oil (0.16, 0.32, and 1.28 ppm), obtained from Petrodar Oil Company. Soil samples were taken from 5 and 10 cm depths after two, 14 and 28 days from crude oil addition. At each sampling date, the recovery of petroleum hydrocarbons measured. Findings – The results obtained indicated that high by significant differences among the different concentrations, different depths and different sampling durations. Moreover, the downward mobility of petroleum hydrocarbons decreased with increasing crude oil concentration. Originality/value – Results of the experiment revealed the importance of studying the fate and mobility of total petroleum hydrocarbons (TPH) in soils in order to facilitate a proper reclamation practice specially in oil polluted sites.

Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Tween 80 ◽  
Arable Soil ◽  
Total Petroleum Hydrocarbons ◽  
Kinetics Of ◽  
Palm Bunch

Total Petroleum Hydrocarbons Categories Concentration in Soils Within the Vicinity Housing Heavy-Duty Diesel Generators in Three Universities in Port Harcourt South-South Nigeria

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Health Hazard ◽  
Soxhlet Extraction ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbons ◽  
Heavy Duty ◽  
Flame Ionization ◽  
Port Harcourt ◽  
Diesel Generators ◽  
Heavy Duty Diesel

Total petroleum hydrocarbons pollution of soil samples randomly collected from three Nigeria Universities in Port Harcourt due to the use of heavy-duty diesel generators was studied to ascertains the level of concentration of the different hydrocarbons’ categories. The soil samples were collected at two different depths of 0.00-0.50m and 0.50-1.00m. The Universities were Ignatius Ajuru University of Education (IAUE), Rivers State University (RSU) and University of Port Harcourt (UNIPORT). The different total petroleum hydrocarbons categories were Gasoline Range Organics (GRO), Diesel Range Organics (DRO) and Lube Oil Range. Soxhlet extraction method was used in extracting the samples and due column clean-up was performed for chromatographic analysis. Gas Chromatography-Flame Ionization Detector was used to determine the level of concentrations of the different categories of total petroleum hydrocarbons. The results showed that at 0.00-0.50m depth, IAUE was 4.42145, 945.4784, and 525.66919 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 494.44799 and 458.6715 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 4.40920, 501.2246 and 467.71426 mg/Kg for GRO, DRO and lube oil range respectively. At 0.50-1.00m depth IAUE was 2.75132, 596.35126, and 311.84451 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 298.06899 and 270.61619 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 2.77780, 301.74701 and 276.88684 mg/Kg for GRO, DRO and lube oil range respectively. The level of soil contamination Showed that GRO > DRO > lube oil range. The observation showed that hydrocarbon pollution decreased with increase in depth. The level of DRO and lube oil range in the studied areas exceeded the limit acceptable and therefore adequate steps should be taken to remedy the situation so that it will not pose any health hazard to the workers operating the heavy-duty generators.

scholarly journals Production of CO2 in crude oil bioremediation in clay soil

2005 ◽  
Vol 48 (spe) ◽  
pp. 249-255 ◽  
Author(s):  
Sandro José Baptista ◽  
Magali Christe Cammarota ◽  
Denize Dias de Carvalho Freire
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Clay Soil ◽  
Fixed Bed ◽  
Bacterial Count ◽  
Fixed Bed Reactor ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Oil And Grease ◽  
Before And After

The aim of the present work was to evaluate the biodegradation of petroleum hydrocarbons in clay soil a 45-days experiment. The experiment was conducted using an aerobic fixed bed reactor, containing 300g of contaminated soil at room temperature with an air rate of 6 L/h. The growth medium was supplemented with 2.5% (w/w) (NH4)2SO4 and 0.035% (w/w) KH2PO4. Biodegradation of the crude oil in the contaminated clay soil was monitored by measuring CO2 production and removal of organic matter (OM), oil and grease (OandG), and total petroleum hydrocarbons (TPH), measured before and after the 45-days experiment, together with total heterotrophic and hydrocarbon-degrading bacterial count. The best removals of OM (50%), OandG (37%) and TPH (45%) were obtained in the bioreactors in which the highest CO2 production was achieved.

scholarly journals Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

2020 ◽  
Vol 7 (2) ◽  
pp. 127-133
Author(s):  
Yalda Basim ◽  
Ghasemali Mohebali ◽  
Sahand Jorfi ◽  
Ramin Nabizadeh ◽  
Mehdi Ahmadi Moghadam ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Environmental Concern ◽  
Bacterial Consortium ◽  
Oil Field ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Flame Ionization ◽  
And Control ◽  
Similar Texture

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.

Efficient biodegradation of naphthalene by a newly characterized indigenous Achromobacter sp. FBHYA2 isolated from Tehran Oil Refinery Complex

2012 ◽  
Vol 66 (3) ◽  
pp. 594-602 ◽  
Author(s):  
Sima Farjadfard ◽  
Seyyed Mehdi Borghei ◽  
Amir Hessam Hassani ◽  
Bagher Yakhchali ◽  
Mehdi Ardjmand ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
Optimal Growth ◽  
Sole Source ◽  
Growth Conditions ◽  
American Petroleum Institute ◽  
Oil Refinery ◽  
Rrna Gene ◽  
Significant Difference ◽  
Aliphatic And Aromatic Hydrocarbons ◽  
Achromobacter Sp

A bacterial strain, FBHYA2, capable of degrading naphthalene, was isolated from the American Petroleum Institute (API) separator of the Tehran Oil Refinery Complex (TORC). Strain FBHYA2 was identified as Achromobacter sp. based on physiological and biochemical characteristics and also phylogenetic similarity of 16S rRNA gene sequence. The optimal growth conditions for strain FBHYA2 were pH 6.0, 30 °C and 1.0% NaCl. Strain FBHYA2 can utilize naphthalene as the sole source of carbon and energy and was able to degrade naphthalene aerobically very fast, 48 h for 96% removal at 500 mg/L concentration. The physiological response of Achromobacter sp., FBHYA2 to several hydrophobic chemicals (aliphatic and aromatic hydrocarbons) was also investigated. No biosurfactant was detected during bacterial growth on any aliphatic/aromatic hydrocarbons. The results of hydrophobicity measurements showed no significant difference between naphthalene- and LB-grown cells. The capability of the strain FBHYA2 to degrade naphthalene completely and rapidly without the need to secrete biosurfactant may make it an ideal candidate to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated sites.

Removal of total petroleum hydrocarbons from oil refinery waste using granulated NaA zeolite nanoparticles modified with hexadecyltrimethylammonium bromide

2016 ◽  
Vol 94 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Betsabe Saremnia ◽  
Akbar Esmaeili ◽  
Mahmoud-Reza Sohrabi
Keyword(s):  
Removal Efficiency ◽  
Flow Rate ◽  
Petroleum Hydrocarbons ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Column Height ◽  
Hexadecyltrimethylammonium Bromide ◽  
Adsorbent Dosage ◽  
Oil Refineries ◽  
Refinery Waste

Petroleum pollution is a common problem in industrial areas to such an extent that it poses a global threat. The wastes of oil refineries contain a wide variety of hydrocarbons. The aim of this study was to investigate the possibility to removal of total petroleum hydrocarbons (TPH) from the Behregan oil refinery, Bushehr, Iran, by granulated nanozeolite NaA particles (NaA-ZNPs) modified with cationic surfactants. Synthesized NaA-ZNPs with a silica source extracted from Hordeum vulgare were granulated by an alginate granulation method and were modified by the cationic surfactant hexadecyltrimethylammonium bromide (CTAB). The CTAB-modified granulated NaA-ZNPs were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and the Brunauer–Emmett–Teller theory. Adsorption of TPH was studied using both a batch process and a continuous-flow process in a fixed-bed column. The effect of various parameters, including time, pH, adsorbent dosage, flow rate, and column height, were investigated and the results were analyzed by gas chromatography – mass spectroscopy. The efficiency of the CTAB-modified granulated NaA-ZNPs was evaluated with one-way analysis of variance software SPSS 21. The highest TPH removal efficiency for batch processing (92.3%) was achieved at an adsorbent dosage 0.5 mg, pH = 7, and 20 min of contact time; the highest TPH removal efficiency for continuous processing (87.4%) was achieved at 15 cm column height and a 17.26 mL/min flow rate. The results indicated the potential of CTAB-modified granulated NaA-ZNPs for absorbing TPH in treating oil refinery waste.

Bioremediation of Soil Contaminated by Crude Oil in Daqing, China

2008 ◽  
Author(s):  
Yiling Zhang ◽  
Dehong Kong ◽  
Sheng Sun ◽  
Zhiguo Luo ◽  
Peng Luo
Keyword(s):  
Moisture Content ◽  
Crude Oil ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Degradation Rate ◽  
Total Petroleum Hydrocarbons ◽  
Optimum Conditions ◽  
Optimum Ratio ◽  
Treatment Unit ◽  
Nutrient Ratio

Bioremediation of soil contaminated by pipeline spills in the Daquin region of China is presented in this paper. The objective of the study was to determine the nutrient ratio required for the bioremediation of soil contaminated by Daqing crude oil. Several experiments were conducted using different proportions of C:N:P:K to choose the optimum ratio of nutrients to be applied for bioremediation, C is supplied from the crude oil in the contaminated soil. The moisture content, porosity, PH and temperature of the contaminated soil were adjusted for optimum conditions. The experimental bioremediation technology was conducted in a treatment unit that was 0.5 m in length, 0.7 m in width, and 1.5 m in height. The results showed that the degradation rate of Total Petroleum Hydrocarbons (TPH) increased with increased concentrations of crude oil in the soil. At the beginning of the bioremediation experiments, the degradation rate of THP is high, but it gradually slowed over the course of the experiment. The degradation rate of TPH averaged 98% over 8 months.

Sign in / Sign up

Export Citation Format

Share Document