scholarly journals Potential of Biosurfactants’ Production on Degrading Heavy Oil by Bacterial Consortia Obtained from Tsunami-Induced Oil-Spilled Beach Areas in Miyagi, Japan

2020 ◽  
Vol 8 (8) ◽  
pp. 577
Author(s):  
Sandia Primeia ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Heavy Oil ◽  
Petroleum Hydrocarbons ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Treatment Plant ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Potential Candidate ◽  
Bacterial Consortia ◽  
Achromobacter Sp ◽  
Degradation Ability

Bioremediation is one of the promising environment-friendly approaches to eliminate oil contamination. However, heavy oil is known to degrade slowly due to its hydrophobicity. Therefore, microorganisms capable of producing biosurfactants are gaining substantial interest because of their potential to alter hydrocarbon properties and thereby speed up the degradation process. In this study, six bacterial consortia were obtained from the oil-spilled beach areas in Miyagi, Japan, and all of which exhibited high potential in degrading heavy oil measured by gas chromatography with flame ionization detector (GC-FID). The polymerase chain reaction—denaturing gradient gel electrophoresis (PCR-DGGE) and next-generation sequencing (NGS) revealed that the diverse microbial community in each consortium changed with subculture and became stable with a few effective microorganisms after 15 generations. The total petroleum hydrocarbons (TPH) degradation ability of the consortia obtained from a former gas station (C1: 81%) and oil refinery company (C6: 79%) was higher than that of the consortia obtained from wastewater treatment plant (WWTP) (C3: 67%, and C5: 73%), indicating that bacteria present in C1 and C6 were historically exposed to petroleum hydrocarbons. Moreover, it was intriguing that the consortium C4, also obtained from WWTP, exhibited high TPH degradation ability (77%). The NGS results revealed that two bacteria, Achromobacter sp. and Ochrobactrum sp., occupied more than 99% of the consortium C4, while no Pseudomonas sp. was found in C4, though this bacterium was observed in other consortia and is also known to be a potential candidate for TPH degradation as reported by previous studies. In addition, the consortium C4 showed high biosurfactant-producing ability among the studied consortia. To date, no study has reported the TPH degradation by the combination of Achromobacter sp. and Ochrobactrum sp.; therefore, the consortium C4 provided an excellent opportunity to study the interaction of and biosurfactant production by these two bacteria during TPH degradation.

scholarly journals Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

2018 ◽  
Vol 20 (2) ◽  
pp. 432-438 ◽  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Sole Source ◽  
Bacterial Consortium ◽  
Soil Samples ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Biochemical Tests ◽  
Polluted Sites ◽  
Achromobacter Sp

In this study, actual soil samples, taken from Kharg Island, Iran, with high levels of total petroleum hydrocarbons (TPHs) were bioremediated by a bacterial consortium. The strains were: Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., Brevibacillus sp. and Staphylococcus sp., which were isolated from two highly polluted sites: Kharg Island and Kermanshah Oil Refinery. The bacteria were identified and confirmed by biochemical tests and the PCR assay. In order to isolate the bacteria, the enrichment method, in which crude oil (2%) was utilized as the sole source of carbon and energy, was used. The results showed that the adapted bacterial consortium could degrade nearly 60% of the pollution. Also, it was found that after 100 days of bioremediation only Brevibacillus sp. could survive in the environment.

Removal of total petroleum hydrocarbons from oil refinery waste using granulated NaA zeolite nanoparticles modified with hexadecyltrimethylammonium bromide

2016 ◽  
Vol 94 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Betsabe Saremnia ◽  
Akbar Esmaeili ◽  
Mahmoud-Reza Sohrabi
Keyword(s):  
Removal Efficiency ◽  
Flow Rate ◽  
Petroleum Hydrocarbons ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Column Height ◽  
Hexadecyltrimethylammonium Bromide ◽  
Adsorbent Dosage ◽  
Oil Refineries ◽  
Refinery Waste

Petroleum pollution is a common problem in industrial areas to such an extent that it poses a global threat. The wastes of oil refineries contain a wide variety of hydrocarbons. The aim of this study was to investigate the possibility to removal of total petroleum hydrocarbons (TPH) from the Behregan oil refinery, Bushehr, Iran, by granulated nanozeolite NaA particles (NaA-ZNPs) modified with cationic surfactants. Synthesized NaA-ZNPs with a silica source extracted from Hordeum vulgare were granulated by an alginate granulation method and were modified by the cationic surfactant hexadecyltrimethylammonium bromide (CTAB). The CTAB-modified granulated NaA-ZNPs were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and the Brunauer–Emmett–Teller theory. Adsorption of TPH was studied using both a batch process and a continuous-flow process in a fixed-bed column. The effect of various parameters, including time, pH, adsorbent dosage, flow rate, and column height, were investigated and the results were analyzed by gas chromatography – mass spectroscopy. The efficiency of the CTAB-modified granulated NaA-ZNPs was evaluated with one-way analysis of variance software SPSS 21. The highest TPH removal efficiency for batch processing (92.3%) was achieved at an adsorbent dosage 0.5 mg, pH = 7, and 20 min of contact time; the highest TPH removal efficiency for continuous processing (87.4%) was achieved at 15 cm column height and a 17.26 mL/min flow rate. The results indicated the potential of CTAB-modified granulated NaA-ZNPs for absorbing TPH in treating oil refinery waste.

Innovative process for total petroleum hydrocarbons reduction on oil refinery sludge through microbubble ozonation

2020 ◽  
Vol 256 ◽  
pp. 120337 ◽  
Author(s):  
Zhiyi Sun ◽  
Fujun Xia ◽  
Ziyang Lou ◽  
Xiaoliang Chen ◽  
Nanwen Zhu ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Oil Refinery ◽  
Total Petroleum Hydrocarbons ◽  
Innovative Process

scholarly journals A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons

2021 ◽  
Vol 12 ◽  
Author(s):  
Simone Becarelli ◽  
Ilaria Chicca ◽  
Salvatore La China ◽  
Giovanna Siracusa ◽  
Alessandra Bardi ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Sole Carbon Source ◽  
Bacterial Species ◽  
Diesel Oil ◽  
Polluted Soil ◽  
Fungal Strain ◽  
Oil Refinery ◽  
Fungal Communities ◽  
Total Petroleum Hydrocarbons ◽  
Fresh Weight

A Ciboria sp. strain (Phylum Ascomycota) was isolated from hydrocarbon-polluted soil of an abandoned oil refinery in Italy. The strain was able to utilize diesel oil as a sole carbon source for growth. Laboratory-scale experiments were designed to evaluate the use of this fungal strain for treatment of the polluted soil. The concentration of total petroleum hydrocarbons (TPH) in the soil was 8,538 mg/kg. Mesocosms containing the contaminated soil were inoculated with the fungal strain at 1 or 7%, on a fresh weight base ratio. After 90 days of incubation, the depletion of TPH contamination was of 78% with the 1% inoculant, and 99% with the 7% inoculant. 16S rDNA and ITS metabarcoding of the bacterial and fungal communities was performed in order to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction indicated Arthrobacter, Dietzia, Brachybacerium, Brevibacterium, Gordonia, Leucobacter, Lysobacter, and Agrobacterium spp. as generalist saprophytes, essential for the onset of hydrocarbonoclastic specialist bacterial species, identified as Streptomyces, Nocardoides, Pseudonocardia, Solirubrobacter, Parvibaculum, Rhodanobacter, Luteiomonas, Planomicrobium, and Bacillus spp., involved in the TPH depletion. The fungal metabolism accelerated the onset of specialist over generalist bacteria. The capacity of the Ciboria sp. to deplete TPH in the soil in treatment was also ascertained.

Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Tween 80 ◽  
Arable Soil ◽  
Total Petroleum Hydrocarbons ◽  
Kinetics Of ◽  
Palm Bunch

Total Petroleum Hydrocarbons Categories Concentration in Soils Within the Vicinity Housing Heavy-Duty Diesel Generators in Three Universities in Port Harcourt South-South Nigeria

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Health Hazard ◽  
Soxhlet Extraction ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbons ◽  
Heavy Duty ◽  
Flame Ionization ◽  
Port Harcourt ◽  
Diesel Generators ◽  
Heavy Duty Diesel

Total petroleum hydrocarbons pollution of soil samples randomly collected from three Nigeria Universities in Port Harcourt due to the use of heavy-duty diesel generators was studied to ascertains the level of concentration of the different hydrocarbons’ categories. The soil samples were collected at two different depths of 0.00-0.50m and 0.50-1.00m. The Universities were Ignatius Ajuru University of Education (IAUE), Rivers State University (RSU) and University of Port Harcourt (UNIPORT). The different total petroleum hydrocarbons categories were Gasoline Range Organics (GRO), Diesel Range Organics (DRO) and Lube Oil Range. Soxhlet extraction method was used in extracting the samples and due column clean-up was performed for chromatographic analysis. Gas Chromatography-Flame Ionization Detector was used to determine the level of concentrations of the different categories of total petroleum hydrocarbons. The results showed that at 0.00-0.50m depth, IAUE was 4.42145, 945.4784, and 525.66919 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 494.44799 and 458.6715 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 4.40920, 501.2246 and 467.71426 mg/Kg for GRO, DRO and lube oil range respectively. At 0.50-1.00m depth IAUE was 2.75132, 596.35126, and 311.84451 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 298.06899 and 270.61619 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 2.77780, 301.74701 and 276.88684 mg/Kg for GRO, DRO and lube oil range respectively. The level of soil contamination Showed that GRO > DRO > lube oil range. The observation showed that hydrocarbon pollution decreased with increase in depth. The level of DRO and lube oil range in the studied areas exceeded the limit acceptable and therefore adequate steps should be taken to remedy the situation so that it will not pose any health hazard to the workers operating the heavy-duty generators.

Fate of Total Petroleum Hydrocarbons in the Environment

2019 ◽  
pp. 57-77 ◽  
Author(s):  
Saranya Kuppusamy ◽  
Naga Raju Maddela ◽  
Mallavarapu Megharaj ◽  
Kadiyala Venkateswarlu
Keyword(s):  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons

An Overview of Total Petroleum Hydrocarbons

2019 ◽  
pp. 1-27 ◽  
Author(s):  
Saranya Kuppusamy ◽  
Naga Raju Maddela ◽  
Mallavarapu Megharaj ◽  
Kadiyala Venkateswarlu
Keyword(s):  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons

Effect of compost in phytoremediation of diesel-contaminated soils

2001 ◽  
Vol 43 (2) ◽  
pp. 291-295 ◽  
Author(s):  
J. Vouillamoz ◽  
M. W. Milke
Keyword(s):  
Moisture Content ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Dilution Effect ◽  
Screening Tests ◽  
Total Petroleum Hydrocarbons ◽  
Controlled Environment ◽  
Grass Growth ◽  
The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

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