Characterization of PIR 32, a candida albicans cell wall protein. (c2009)

2009 ◽  
Author(s):  
Wael G. Bahnan
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein

scholarly journals Characterization of Hwp2, a Candida albicans putative GPI-anchored cell wall protein necessary for invasive growth

2010 ◽  
Vol 165 (3) ◽  
pp. 250-258 ◽  
Author(s):  
Peter Hayek ◽  
Leila Dib ◽  
Pascal Yazbeck ◽  
Berna Beyrouthy ◽  
Roy A. Khalaf
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein ◽  
Invasive Growth

Characterization of a disulphide-bound Pir-cell wall protein (Pir-CWP) ofYarrowia lipolytica

Yeast ◽  
2003 ◽  
Vol 20 (5) ◽  
pp. 417-426 ◽  
Author(s):  
Lahcen Jaafar ◽  
Isma�l Moukadiri ◽  
Jes�s Zueco
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein

scholarly journals Isolation and Characterization of a Proline-Rich Cell Wall Protein from Soybean Seedlings

1990 ◽  
Vol 94 (4) ◽  
pp. 1897-1902 ◽  
Author(s):  
Susan M. Kleis-San Francisco ◽  
Mary L. Tierney
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein ◽  
Soybean Seedlings ◽  
Isolation And Characterization

Characterization of a major cell wall antigen and potential adhesin in three strains of Candida albicans

1994 ◽  
Vol 162 (1-2) ◽  
pp. 33-40
Author(s):  
W. Jenq ◽  
Chi Lan Chen ◽  
Chun Chang ◽  
Richard F. E. Crang
Keyword(s):  
Cell Wall ◽  
Candida Albicans

scholarly journals Kinetic and thermodynamic characterization of the interactions between the components of human plasma kinin-forming system and isolated and purified cell wall proteins of Candida albicans

2015 ◽  
Vol 62 (4) ◽  
pp. 825-835 ◽  
Author(s):  
Karolina Seweryn ◽  
Justyna Karkowska-Kuleta ◽  
Natalia Wolak ◽  
Oliwia Bochenska ◽  
Sylwia Kedracka-Krok ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Human Plasma ◽  
Cell Wall Proteins ◽  
Thermodynamic Characterization ◽  
Plasma Kinin

Characterization of the recombinant Candida albicans β-1,2-mannosyltransferase that initiates the β-mannosylation of cell wall phosphopeptidomannan

2013 ◽  
Vol 457 (2) ◽  
pp. 347-360 ◽  
Author(s):  
Emeline Fabre ◽  
Ghenima Sfihi-Loualia ◽  
Marilyne Pourcelot ◽  
Bernadette Coddeville ◽  
Frédéric Krzewinski ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Immunocompromised Patients ◽  
Serious Infections ◽  
Antigenic Factor

Candida albicans is a yeast responsible for serious infections in immunocompromised patients. In the present paper, we report the identification of an enzyme that initiates the synthesis of a bioactive cell wall carbohydrate antigenic factor, the β-mannans.

Production and characterization of monoclonal antibodies to the sapstaining fungus Ophiostoma piceae

1994 ◽  
Vol 40 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Srabani Banerjee ◽  
Judy Little ◽  
Maria Chan ◽  
Brian T. Luck ◽  
Colette Breuil ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Light And Electron Microscopy ◽  
Cross Reactivity ◽  
Cell Wall Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzymatic Modification ◽  
Ophiostoma Piceae

A sensitive immunological tool has been developed to detect the sapstaining fungus Ophiostoma piceae 3871, which plagues the wood industry. Monoclonal antibodies (1F3(1), 4G3(14), 4G2(4), and 2B6(24)) produced against cell wall protein extracts of this fungus were specific. Specificity was estimated by enzyme linked immunosorbent assay, western blotting, and light and electron microscopy using the immunogold technique. Electron microscopy revealed gold particles localized on the outer surface of the cell wall. When screened against 24 biological control fungi the antibodies showed pratically no cross-reactivity (< 4%). When tested against 19 other staining fungi, the antibodies recognized three strains of Ophiostoma piceae, 1F3(1) recognized Phialophora botulispora, and the antibodies showed less than 5% reactivity with the other fungi. Chemical and enzymatic modification of the antigen revealed that the epitopes recognized by the monoclonal antibodies were glycospecific. Although the antibodies were produced against the cell wall protein extracts of the fungus grown in liquid culture, they also recognized the fungus growing in wood and therefore can be employed to investigate wood colonization by this fungus.Key words: Ophiostoma piceae, monoclonal antibodies, glycoprotein.

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