Characterization of a disulphide-bound Pir-cell wall protein (Pir-CWP) ofYarrowia lipolytica

Yeast ◽  
2003 ◽  
Vol 20 (5) ◽  
pp. 417-426 ◽  
Author(s):  
Lahcen Jaafar ◽  
Isma�l Moukadiri ◽  
Jes�s Zueco
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein

scholarly journals Isolation and Characterization of a Proline-Rich Cell Wall Protein from Soybean Seedlings

1990 ◽  
Vol 94 (4) ◽  
pp. 1897-1902 ◽  
Author(s):  
Susan M. Kleis-San Francisco ◽  
Mary L. Tierney
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein ◽  
Soybean Seedlings ◽  
Isolation And Characterization

Production and characterization of monoclonal antibodies to the sapstaining fungus Ophiostoma piceae

1994 ◽  
Vol 40 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Srabani Banerjee ◽  
Judy Little ◽  
Maria Chan ◽  
Brian T. Luck ◽  
Colette Breuil ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Light And Electron Microscopy ◽  
Cross Reactivity ◽  
Cell Wall Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzymatic Modification ◽  
Ophiostoma Piceae

A sensitive immunological tool has been developed to detect the sapstaining fungus Ophiostoma piceae 3871, which plagues the wood industry. Monoclonal antibodies (1F3(1), 4G3(14), 4G2(4), and 2B6(24)) produced against cell wall protein extracts of this fungus were specific. Specificity was estimated by enzyme linked immunosorbent assay, western blotting, and light and electron microscopy using the immunogold technique. Electron microscopy revealed gold particles localized on the outer surface of the cell wall. When screened against 24 biological control fungi the antibodies showed pratically no cross-reactivity (< 4%). When tested against 19 other staining fungi, the antibodies recognized three strains of Ophiostoma piceae, 1F3(1) recognized Phialophora botulispora, and the antibodies showed less than 5% reactivity with the other fungi. Chemical and enzymatic modification of the antigen revealed that the epitopes recognized by the monoclonal antibodies were glycospecific. Although the antibodies were produced against the cell wall protein extracts of the fungus grown in liquid culture, they also recognized the fungus growing in wood and therefore can be employed to investigate wood colonization by this fungus.Key words: Ophiostoma piceae, monoclonal antibodies, glycoprotein.

scholarly journals Characterization of Hwp2, a Candida albicans putative GPI-anchored cell wall protein necessary for invasive growth

2010 ◽  
Vol 165 (3) ◽  
pp. 250-258 ◽  
Author(s):  
Peter Hayek ◽  
Leila Dib ◽  
Pascal Yazbeck ◽  
Berna Beyrouthy ◽  
Roy A. Khalaf
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Wall Protein ◽  
Invasive Growth

scholarly journals Characterization of Carrot Cell Wall Protein

1981 ◽  
Vol 68 (4) ◽  
pp. 956-963 ◽  
Author(s):  
Macky A. Smith
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein ◽  
Carrot Cell

scholarly journals Cloning and characterization of the 5' region of the cell wall protein gene operon in Bacillus brevis 47.

1987 ◽  
Vol 169 (3) ◽  
pp. 1239-1245 ◽  
Author(s):  
H Yamagata ◽  
T Adachi ◽  
A Tsuboi ◽  
M Takao ◽  
T Sasaki ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Gene ◽  
Cell Wall Protein ◽  
Bacillus Brevis

scholarly journals Characterization of Carrot Cell Wall Protein

1981 ◽  
Vol 68 (4) ◽  
pp. 964-968 ◽  
Author(s):  
Macky A. Smith
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein ◽  
Carrot Cell
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