scholarly journals Isolation and Characterization of a Proline-Rich Cell Wall Protein from Soybean Seedlings

1990 ◽  
Vol 94 (4) ◽  
pp. 1897-1902 ◽  
Author(s):  
Susan M. Kleis-San Francisco ◽  
Mary L. Tierney
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein ◽  
Soybean Seedlings ◽  
Isolation And Characterization

scholarly journals 586 Cell Wall Protein Differences in Fruits of Two Tabasco Pepper Lines

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 497D-497
Author(s):  
Ramon A. Arancibia ◽  
Carl E. Motsenbocker
Keyword(s):  
Cell Wall ◽  
Protein Profile ◽  
Cell Wall Protein ◽  
Abscission Zone ◽  
Capsicum Frutescens ◽  
Factors Affecting ◽  
Fruit Abscission ◽  
Detachment Force ◽  
Isolation And Characterization

`McIlhenny Select' (easy detachment) and `Hard Pick' are two lines of tabasco pepper (Capsicum frutescens L.) that differ in the fruit detachment characteristics. Cellulase (Cx) and polygalacturonase (PG) activity, extracted from the fruit abscission zone, correlated inversely with the force needed to separate the fruit from the pedicel. A trend of higher Cx and PG is associated with the lower detachment force in the McIlhenny Select line. Differences in the fruit cell wall protein profile between both lines occurred during ripening. Two bands of 23 kDa and 40 kDa were higher in `McIlhenny Select'. A band of approximately 30 kDa was higher in `Hard Pick', while a band of ≈70 kDa increased in both lines. Isolation and characterization of these bands as well as Cx and PG is needed to understand the factors affecting fruit detachment in tabasco pepper.

Isolation and Characterization of Plant Cell Walls and Cell Wall Components

1988 ◽  
pp. 3-40 ◽  
Author(s):  
WILLIAM S. YORK ◽  
ALAN G. DARVILL ◽  
MICHAEL MCNEIL ◽  
THOMAS T. STEVENSON ◽  
PETER ALBERSHEIM
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Cell Wall Components ◽  
Isolation And Characterization ◽  
Wall Components

Isolation and characterization of plant cell walls and cell wall components

1986 ◽  
pp. 3-40 ◽  
Author(s):  
William S. York ◽  
Alan G. Darvill ◽  
Michael McNeil ◽  
Thomas T. Stevenson ◽  
Peter Albersheim
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Cell Wall Components ◽  
Isolation And Characterization ◽  
Wall Components

The role of Golgi bodies in polysaccharide sulphation in Fucuszygotes

1978 ◽  
Vol 32 (1) ◽  
pp. 337-356
Author(s):  
M.E. Callow ◽  
S.J. Coughlan ◽  
L.V. Evans
Keyword(s):  
Cell Wall ◽  
Alginic Acid ◽  
Soluble Fraction ◽  
Amorphous Layer ◽  
Acceptor Molecule ◽  
Golgi Bodies ◽  
Isolation And Characterization ◽  
Fucus Serratus

The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers—an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pole. Light- and electron-microscope autoradiographic pulse-chase experiments on 22-h zygotes using 35SO2-(4) show the Golgi bodies to be the sites of fucan sulphation. The isolation and characterization of isolated Golgi-rich fractions from 22-h zygotes shows that the first detectable labelled macromolecule is associated with these fractions 2 min after addition of 35SO2-(4). The sulphate acceptor molecule has been partially characterized. 35S-APS and 35S-paps are detectable in the soluble fraction 0.5 min after addition of 35SO2-(4). The results are discussed in relation to other published work on the differentiation of Fucus embryos and on polysaccharide sulphation.

Characterization of a disulphide-bound Pir-cell wall protein (Pir-CWP) ofYarrowia lipolytica

Yeast ◽  
2003 ◽  
Vol 20 (5) ◽  
pp. 417-426 ◽  
Author(s):  
Lahcen Jaafar ◽  
Isma�l Moukadiri ◽  
Jes�s Zueco
Keyword(s):  
Cell Wall ◽  
Cell Wall Protein

Production and characterization of monoclonal antibodies to the sapstaining fungus Ophiostoma piceae

1994 ◽  
Vol 40 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Srabani Banerjee ◽  
Judy Little ◽  
Maria Chan ◽  
Brian T. Luck ◽  
Colette Breuil ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Light And Electron Microscopy ◽  
Cross Reactivity ◽  
Cell Wall Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzymatic Modification ◽  
Ophiostoma Piceae

A sensitive immunological tool has been developed to detect the sapstaining fungus Ophiostoma piceae 3871, which plagues the wood industry. Monoclonal antibodies (1F3(1), 4G3(14), 4G2(4), and 2B6(24)) produced against cell wall protein extracts of this fungus were specific. Specificity was estimated by enzyme linked immunosorbent assay, western blotting, and light and electron microscopy using the immunogold technique. Electron microscopy revealed gold particles localized on the outer surface of the cell wall. When screened against 24 biological control fungi the antibodies showed pratically no cross-reactivity (< 4%). When tested against 19 other staining fungi, the antibodies recognized three strains of Ophiostoma piceae, 1F3(1) recognized Phialophora botulispora, and the antibodies showed less than 5% reactivity with the other fungi. Chemical and enzymatic modification of the antigen revealed that the epitopes recognized by the monoclonal antibodies were glycospecific. Although the antibodies were produced against the cell wall protein extracts of the fungus grown in liquid culture, they also recognized the fungus growing in wood and therefore can be employed to investigate wood colonization by this fungus.Key words: Ophiostoma piceae, monoclonal antibodies, glycoprotein.

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