scholarly journals Microsatellite DNA polymorphism in selectively controlled Apis mellifera carnica and Apis mellifera caucasica populations from Poland

2015 ◽  
Vol 67 (3) ◽  
pp. 889-894 ◽  
Author(s):  
Stanimila Nikolova ◽  
Malgorzata Bienkowska ◽  
Dariusz Gerula ◽  
Evgeniya Ivanova
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Dna Polymorphism ◽  
Dna Analysis ◽  
Apis Mellifera Carnica ◽  
Expected Heterozygosity ◽  
Apis Mellifera Caucasica ◽  
The Mean ◽  
Private Alleles

Genetic polymorphism in selectively controlled honeybee populations of A. m. carnica and A. m. caucasica in Poland, was characterized by microsatellite DNA analysis. All honeybee samples were analyzed for nine microsatellite loci: Ac011; A024; A043; A088; Ap226; Ap238; Ap243; Ap249 and Ap256, which were found to be polymorphic in both populations. The mean number of alleles per locus was 6.222 for A. m. carnica and 4.556 for A. m. caucasica. Average observed and expected heterozygosity values were calculated as 0.976 and 0.734 in A. m. carnica and as 0.933 and 0.603 in A. m. caucasica, respectively. For the nine microsatellite loci, a total of 76 alleles were found in both populations. Thirty-five private alleles were observed in A. m. carnica and 20 in A. m. caucasica. Information about allele frequencies, FST values and genotypic differentiation is given. Nei?s genetic distance between studied populations of A. m. carnica and A. m. caucasica was calculated as 0.384.

Genetic structure of Apis mellifera carnica in Slovakia based on microsatellite DNA polymorphism

Biologia ◽  
2017 ◽  
Vol 72 (11) ◽  
Author(s):  
Matúš Šťastný ◽  
Jaroslav Gasper ◽  
Miroslav Bauer
Keyword(s):  
Apis Mellifera ◽  
Genetic Structure ◽  
Microsatellite Dna ◽  
Dna Polymorphism ◽  
Apis Mellifera Carnica

AbstractCarniolan honeybee (

scholarly journals Observation by microsatellite DNA analysis of sperm usage in naturally mated honeybee queens (Apis mellifera ligustica) over a period of two years

2008 ◽  
Vol 7 (4) ◽  
pp. 465-478
Author(s):  
Cristina Previtali ◽  
Graziella Bongioni ◽  
Cecilia Costa ◽  
Marco Lodesani ◽  
Andrea Galli
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Dna ◽  
Dna Analysis ◽  
Apis Mellifera Ligustica

scholarly journals Susceptibility of Bee Larvae to Chalkbrood in Relation to Hygienic Behaviour of Worker Bees in Colonies of Chosen Races of Honeybee (Apis Mellifera )

2014 ◽  
Vol 58 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Beata Panasiuk ◽  
Małgorzata Bieńkowska ◽  
Dariusz Gerula ◽  
Paweł Węgrzynowicz
Keyword(s):  
Apis Mellifera ◽  
Correlation Coefficient ◽  
Field Conditions ◽  
Ascosphaera Apis ◽  
Hygienic Behaviour ◽  
Apis Mellifera Carnica ◽  
Apis Mellifera Mellifera ◽  
Apis Mellifera Caucasica ◽  
P Colonies ◽  
Bee Colonies

Abstract The susceptibility of bee larvae to Ascosphaera apis infestation and the hygienic behaviour of worker bees in relation to A. apis infected and freeze-killed brood were evaluated in three races of bees: Apis mellifera carnica, Apis mellifera caucasica, and Apis mellifera mellifera. Experimental bee colonies were evaluated in field conditions during the three beekeeping seasons. The lowest percentage of infected larvae was observed in car GR1 and mel A colonies (8.5% and 15%, respectively) and the highest in car Mr and cau P colonies (21% and 24.3%, respectively). Bees in the car GR1 and mel A colonies removed mummified brood in a shorter period of time (6.5 and 7.1 days on average, respectively) than car Mr and cau P colonies (above 8 days). Bees in the mel A and car GR1 colonies cleaned significantly more cells with freeze-killed brood within 24 and 48 hours (above 70% and 80% on average, respectively) than car Mr and cau P colonies (on average 10 - 20% lower cleaning rate). A low correlation coefficient was found for the susceptibility of larvae to A. apis infection and hygienic behaviour.

scholarly journals  Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 345-352 ◽  
Author(s):  
D. Kaczmarczyk ◽  
M. Luczynski ◽  
P. Brzuzan
Keyword(s):  
Genetic Variation ◽  
Mississippi River ◽  
Microsatellite Dna ◽  
Dna Analysis ◽  
Adult Fish ◽  
Native Population ◽  
Distance Measurements ◽  
Polyodon Spathula ◽  
The Usa ◽  
The Mean

There are two stocks of American paddlefish Polyodon spathula in Poland, one in Pogorze and the other in Wasosze. These stocks were established from small quantities of eggs imported from the USA in 1995. In this study, we examined genetic variation at seven microsatellite loci in adult fish from the two Polish farms and one stock farmed in Gorny Tykich in Ukraine. Our data were compared with those reported for one native population from the Mississippi River (USA). The polymorphism of examined loci varied in the Polish and Ukrainian stocks, showing 25&minus;30 alleles across each stock (3.6&ndash;4.3 alleles per locus), though they were less polymorphic than those in the Mississippi (49 across the population, 7.0 per locus). The mean observed heterozygosity per locus estimated in Polish stocks (0.59&minus;0.60) was comparable to that found in the Mississippi (0.68). The Garza-Williamson index and values of the heterozygosity excesses revealed a reduction of genetic variation in all the three European stocks (probably resulting from bottleneck or founder effect). Finally, genetic distance measurements confirmed a closer relationship between Pogorze and Wasosze <br />(F<sub>ST </sub>= 0.007) stocks than between these two Polish stocks and that from Ukraine (F<sub>ST</sub> = 0.096 and F<sub>ST </sub>= 0.054). &nbsp;

scholarly journals Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

2018 ◽  
Vol 19 (4) ◽  
pp. 1593-1600
Author(s):  
SUTARNO . ◽  
NINA KURNIANINGRUM ◽  
ELISA HERAWATI ◽  
AHMAD DWI SETYAWAN
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Dna Markers ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Polymorphic Information Content ◽  
Entire Genome ◽  
Genetic Characteristics ◽  
Microsatellite Dna Markers ◽  
The Mean

Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Gradecattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensivelyemployed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markersare short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. Thepurpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattleusing microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to WizardGenomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824,ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENEprogram version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Maduracattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Maduracattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) ofOngole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogethersuggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value>0.5 especially for intraspecies of Madura cattle.

scholarly journals Genetic diversity of different breeds of Kazakh sheep using microsatellite analysis

2019 ◽  
Vol 62 (1) ◽  
pp. 305-312
Author(s):  
Kairat Dossybayev ◽  
Zarina Orazymbetova ◽  
Aizhan Mussayeva ◽  
Naruya Saitou ◽  
Rakhymbek Zhapbasov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Microsatellite Loci ◽  
Allelic Diversity ◽  
Genetic Distances ◽  
Sheep Breed ◽  
Expected Heterozygosity ◽  
The Mean ◽  
High Level ◽  
Short Tandem

Abstract. A total of 75 individuals from five sheep populations in Kazakhstan were investigated based on 12 STR (short tandem repeat, also known as microsatellite) markers in order to study their genetic structure and phylogenetic relationship based on genetic distances. These sheep had a high level of genetic diversity. In total, 163 alleles were found in all the populations using 12 microsatellite loci. The mean number of alleles, effective number of alleles, and polymorphism information content (PIC) values per loci were 13.4, 5.9, and 0.78, respectively. Comparing the allelic diversity between the populations, the highest genetic diversity was observed in the Edilbay-1 sheep breed (8.333±0.644), and the lowest parameter was for Kazakh Arkhar-Merino (7.083±0.633). In all populations, there is a deficiency of heterozygosity. The largest genetic diversity was found in loci INRA023 and CSRD247 with 16 alleles, and the smallest polymorphism was noted for the locus D5S2 with 8 alleles. The level of observed heterozygosity was in the range 0.678±0.051 for Kazakh Arkhar-Merino and 0.767±0.047 for Kazakh fat-tailed coarse wool. The expected heterozygosity level range was from 0.702±0.033 for Kazakh Arkhar-Merino to 0.777±0.023 for Edilbay-1. When 12 microsatellite loci are compared, the OarFCB20 locus showed the highest level of genetic variability. Excess of heterozygosity was observed at three loci; MAF065, McM042, and OarFCB20. The highest genetic distance was observed between Kazakh Arkhar-Merino and Edilbay-1, whereas the genetic distance between Edilbay-1 and Edilbay-2 is the smallest using Nei's standard genetic distance. The Edilbay-1 sheep breed possesses the largest genetic diversity among these five populations.

Microsatellite DNA analysis of parapatric lamprey (Entosphenus spp.) populations: implications for evolution, taxonomy, and conservation of a Canadian endemic

2012 ◽  
Vol 90 (3) ◽  
pp. 291-303 ◽  
Author(s):  
Eric B. Taylor ◽  
Les N. Harris ◽  
Erin K. Spice ◽  
Margaret F. Docker
Keyword(s):  
British Columbia ◽  
Life History ◽  
Genetic Differentiation ◽  
Microsatellite Dna ◽  
Species Complex ◽  
Dna Analysis ◽  
Distinct Species ◽  
Population Subdivision ◽  
Pacific Lamprey ◽  
Expected Heterozygosity

Parapatric freshwater and anadromous parasitic lampreys (Petromyzontiformes) from southwestern Vancouver Island, British Columbia, have been described as distinct taxa (Vancouver lamprey ( Entosphenus macrostomus (Beamish, 1982)) and Pacific lamprey ( Entosphenus tridentatus (Richardson, 1836)), respectively), using morphology, life history, and physiology. We tested for genetic differentiation at microsatellite DNA loci between these taxa and similar freshwater parasitic lampreys from two other lakes. The number of alleles and expected heterozygosity averaged 3.8 and 0.50, respectively, across loci and populations, and anadromous populations were more variable than freshwater populations. Population subdivision was moderate (FST = 0.096, P < 0.001) and 3% of the total variation was found between taxa and 1.7% was found among populations within taxa (both P < 0.001). Parapatric freshwater and anadromous parasitic lampreys separated by a maximum of 40 km were more distinct (mean FST = 0.042) than were anadromous populations located 800 km from one another (mean FST = 0.012). Localities within lakes with parasitic freshwater lampreys, however, showed little differentiation (FST = 0.0–0.08). Our data support recognizing E. macrostomus and E. tridentatus as distinct species, but similar levels of differentiation between these taxa and other freshwater parasitic lampreys suggest a species complex where the taxonomy remains unclear.

Isolation, via 454 sequencing, and characterisation of microsatellites for Phalacrocorax fuscescens, the black-faced cormorant (Aves : Phalacrocoracidae)

2012 ◽  
Vol 60 (5) ◽  
pp. 340
Author(s):  
Julie Riordan ◽  
Michael G. Gardner ◽  
Alison J. Fitch ◽  
Gregory R. Johnston
Keyword(s):  
Family Structure ◽  
Microsatellite Loci ◽  
Genomic Dna ◽  
454 Sequencing ◽  
South Australia ◽  
Breeding Colony ◽  
Polymorphic Loci ◽  
Expected Heterozygosity ◽  
Amplification Success ◽  
The Mean

The black-faced cormorant, Phalacrocorax fuscescens, is a brood-reducing seabird endemic to the southern waters of Australia. Microsatellite loci were isolated from genomic DNA using 454 shotgun sequencing. Thirty-one loci were tested and, of these, 16 were found to be polymorphic. Further characterisation was conducted on seven loci that were genotyped in 42 adult individuals from a single breeding colony in South Australia. The number of alleles per locus ranged from three to eight (s.d. ± 2.16), and the mean observed and expected heterozygosity was 0.66 (s.d. ± 0.249) and 0.62 (s.d. ± 0.178) respectively. We confirm that four loci conformed to Hardy–Weinberg expectations. Four other Phalacrocorax species were trialled for amplification of these four polymorphic loci. Amplification success varied between loci and species. These loci will be useful in determining genetic family structure and exploring nestling relatedness to further understand how relatedness influences competitive behaviours in brood-reducing species.

scholarly journals Analysis of genetic polymorphism in six meat sheep breeds and genetic distances between them

2009 ◽  
Vol 54 (No. 10) ◽  
pp. 461-467
Author(s):  
R.J. Chen ◽  
Z.P. Yang ◽  
D.J. Ji ◽  
D.Y. Qu ◽  
Y.L. Li ◽  
...  
Keyword(s):  
Dna Markers ◽  
Microsatellite Dna ◽  
Female Parent ◽  
Genetic Distances ◽  
Inbreeding Coefficient ◽  
Expected Heterozygosity ◽  
Microsatellite Dna Markers ◽  
Tan Sheep ◽  
The Mean ◽  
Meat Sheep

The genomes of 6 sheep populations (Dorset, Texel, Black-Suffolk, Tan sheep, Small-tailed Han sheep, Tan and Small-tailed Han hybrid sheep) were screened using 7 microsatellite DNA markers to estimate the genetic diversities and genetic distances among these populations. About 105 alleles were detected at 7 loci in 6 populations. The average observed and expected heterozygosity ranged from 0.2901 to 0.4534 and from 0.8007 to 0.8737 in 6 sheep populations. The expected heterozygosity of each population was much higher than the observed heterozygosity. The mean polymorphism information content (<I>PIC</I>) value of populations ranged from 0.7188 to 0.8546. The coefficient of gene differentiation (<I>F</I><sub>st</sub>) between populations was very low (6.98%). The percentage of inbreeding coefficient for all populations (<I>F</I><sub>it</sub>) was 56.02%, while within breeds (<I>F</I><sub>is</sub>) it was 52.72%. Assuming that heterosis could be estimated on the basis of genetic distances between examined breeds, the hybrid of Tan and Small-tailed Han sheep could be designated as the best female parent, followed by Tan sheep and Small-tailed Han sheep.

Genetic Diversity of Six Duck Populations in India

2021 ◽  
Author(s):  
P. Veeramani ◽  
R. Prabakaran ◽  
S.N. Sivaselvam ◽  
T. Sivakumar ◽  
S.T. Selvan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Tamil Nadu ◽  
Expected Number ◽  
Pekin Ducks ◽  
Expected Heterozygosity ◽  
Chicken Population ◽  
The Mean ◽  
Substantial Degree

Background: The variety of indigenous duck germplasm contribute maximum to the poultry industry in India, besides chicken population. In southern part of India, the available duck genetic resource, particularly Arni ducks (comprises of Sanyasi and Keeri ducks) of Tamil Nadu, has its own characteristics with innate potentiality of higher productivity without any input system of management. Genetic characterization and diversity of indigenous duck genetic resources has not been properly studied. In the present study, the genetic diversity of Arni ducks with other indigenous and exotic duck germplasm were analysed with microsatellite markers. Method: Genomic DNA was isolated from the blood samples of six duck populations. Molecular characterization was carried out with duck specific FAO recommended microsatellite markers. The genotyping of ducks was done based on the size of 4324 PCR amplicons of 23 microsatellite loci, which were subjected to capillary electrophoresis using automatic sequencer.Result: A total of 222 alleles in six duck populations across 23 microsatellite loci with a mean of 9.65 ± 0.95 alleles were found. Kuttanad duck variety had the highest number of alleles (139) followed by Sanyasi (136), Keeri (129), Muscovy (118), Assam (91) and White Pekin (78) ducks. The mean observed number of alleles was 6.04 ± 0.59, 5.91 ± 0.76, 5.61 ± 3.17, 5.13 ± 0.44, 3.96 ± 0.76 and 3.39 ± 0.40 in Kuttanad, Sanyasi, Keeri, Muscovy, Assam and White Pekin ducks respectively. The overall mean polymorphism information content (PIC) values among the six duck populations was 0.6269. In most of the duck populations, the mean PIC value was more than 0.5 except in Assam (0.4815) and White Pekin (0.3725) ducks. The observed heterozygosity was the highest in Keeri ducks (0.5217) and lowest in White Pekin ducks (0.2766), while, the mean expected heterozygosity was the highest in Sanyasi (0.5628) and lowest in White Pekin (0.4038) ducks. The variations in the observed and expected number of alleles, differences in PIC of various microsatellite loci might be attributed to the genetic variability of the duck populations, number and type of microsatellite primers utilised for analysis and the genetic diversity of the duck breeds under study. Higher FST value indicates the substantial degree of breed differentiation among the studied duck populations.

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