Microsatellite DNA analysis of parapatric lamprey (Entosphenus spp.) populations: implications for evolution, taxonomy, and conservation of a Canadian endemic

2012 ◽  
Vol 90 (3) ◽  
pp. 291-303 ◽  
Author(s):  
Eric B. Taylor ◽  
Les N. Harris ◽  
Erin K. Spice ◽  
Margaret F. Docker
Keyword(s):  
British Columbia ◽  
Life History ◽  
Genetic Differentiation ◽  
Microsatellite Dna ◽  
Species Complex ◽  
Dna Analysis ◽  
Distinct Species ◽  
Population Subdivision ◽  
Pacific Lamprey ◽  
Expected Heterozygosity

Parapatric freshwater and anadromous parasitic lampreys (Petromyzontiformes) from southwestern Vancouver Island, British Columbia, have been described as distinct taxa (Vancouver lamprey ( Entosphenus macrostomus (Beamish, 1982)) and Pacific lamprey ( Entosphenus tridentatus (Richardson, 1836)), respectively), using morphology, life history, and physiology. We tested for genetic differentiation at microsatellite DNA loci between these taxa and similar freshwater parasitic lampreys from two other lakes. The number of alleles and expected heterozygosity averaged 3.8 and 0.50, respectively, across loci and populations, and anadromous populations were more variable than freshwater populations. Population subdivision was moderate (FST = 0.096, P < 0.001) and 3% of the total variation was found between taxa and 1.7% was found among populations within taxa (both P < 0.001). Parapatric freshwater and anadromous parasitic lampreys separated by a maximum of 40 km were more distinct (mean FST = 0.042) than were anadromous populations located 800 km from one another (mean FST = 0.012). Localities within lakes with parasitic freshwater lampreys, however, showed little differentiation (FST = 0.0–0.08). Our data support recognizing E. macrostomus and E. tridentatus as distinct species, but similar levels of differentiation between these taxa and other freshwater parasitic lampreys suggest a species complex where the taxonomy remains unclear.

Microsatellite DNA analysis of coastal populations of bull trout (Salvelinus confluentus) in British Columbia: zoogeographic implications and its application to recreational fishery management

2006 ◽  
Vol 63 (5) ◽  
pp. 1157-1171 ◽  
Author(s):  
Eric B Taylor ◽  
A B Costello
Keyword(s):  
British Columbia ◽  
Microsatellite Dna ◽  
Fishery Management ◽  
Dna Analysis ◽  
Population Subdivision ◽  
Fraser River ◽  
Bull Trout ◽  
Salvelinus Confluentus ◽  
Recreational Fishery ◽  
Skeena River

Microsatellite DNA variation was assayed among 383 bull trout (Salvelinus confluentus) from 20 Pacific coastal localities from the Skeena River (central British Columbia) to the Olympic Peninsula (western Washington State). An average of 1.7 alleles was resolved per population and heterozygosity averaged 0.35. Twenty-six fish were identified as bull trout × Dolly Varden (Salvelinus malma) hybrids. Population subdivision was substantial (θ = 0.33), but subdivision was higher (θ = 0.46) when interior populations (N = 37) were included, indicating a major genetic distinction between "coastal" and "interior" bull trout. Bull trout populations north of the Squamish River were genetically more similar to interior bull trout than to other more southern coastal populations, suggesting that they had been founded by headwater transfers from interior populations. Individual assignment of bull trout averaged 53.4% correct assignment among populations (range: 12%–95%). Mixture analysis indicated that most fish from the lower Fraser River recreational fishery originated from major nearby tributaries (e.g., Pitt, upper Lillooet, and Chilliwack rivers). Our results substantiate the existence of two major evolutionary lineages of bull trout and highlight the importance of tributary habitats for the persistence of local populations, as well as for those that forage in downstream areas on the lower Fraser River.

Genetic Differentiation among Sympatric Migratory and Resident Life History Forms of Rainbow Trout in British Columbia

2008 ◽  
Vol 137 (4) ◽  
pp. 1268-1277 ◽  
Author(s):  
Daniel D. Heath ◽  
Corwyn M. Bettles ◽  
Sara Jamieson ◽  
Iga Stasiak ◽  
Margaret F. Docker
Keyword(s):  
British Columbia ◽  
Life History ◽  
Rainbow Trout ◽  
Genetic Differentiation

scholarly journals Microsatellite DNA polymorphism in selectively controlled Apis mellifera carnica and Apis mellifera caucasica populations from Poland

2015 ◽  
Vol 67 (3) ◽  
pp. 889-894 ◽  
Author(s):  
Stanimila Nikolova ◽  
Malgorzata Bienkowska ◽  
Dariusz Gerula ◽  
Evgeniya Ivanova
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Dna Polymorphism ◽  
Dna Analysis ◽  
Apis Mellifera Carnica ◽  
Expected Heterozygosity ◽  
Apis Mellifera Caucasica ◽  
The Mean ◽  
Private Alleles

Genetic polymorphism in selectively controlled honeybee populations of A. m. carnica and A. m. caucasica in Poland, was characterized by microsatellite DNA analysis. All honeybee samples were analyzed for nine microsatellite loci: Ac011; A024; A043; A088; Ap226; Ap238; Ap243; Ap249 and Ap256, which were found to be polymorphic in both populations. The mean number of alleles per locus was 6.222 for A. m. carnica and 4.556 for A. m. caucasica. Average observed and expected heterozygosity values were calculated as 0.976 and 0.734 in A. m. carnica and as 0.933 and 0.603 in A. m. caucasica, respectively. For the nine microsatellite loci, a total of 76 alleles were found in both populations. Thirty-five private alleles were observed in A. m. carnica and 20 in A. m. caucasica. Information about allele frequencies, FST values and genotypic differentiation is given. Nei?s genetic distance between studied populations of A. m. carnica and A. m. caucasica was calculated as 0.384.

Extinction of a Population of Anadromous Parasitic Lamprey, Lampetra tridentata, Upstream of an Impassable Dam

1989 ◽  
Vol 46 (3) ◽  
pp. 420-425 ◽  
Author(s):  
R. J. Beamish ◽  
T. G. Northcote
Keyword(s):  
British Columbia ◽  
Life History ◽  
Young Adult ◽  
Age Determination ◽  
Dam Construction ◽  
Pacific Lamprey ◽  
High Incidence ◽  
Adult Lamprey ◽  
Lampetra Tridentata

The construction of dams on the outlet of Elsie Lake, in British Columbia, prevented young adult Pacific lamprey (Lampetra tridentata) from going to sea and adult lamprey from spawning above the dams. This resulted in a high incidence of lamprey attacks on resident trout that was initially interpreted as indicating that the lamprey population was landlocked. This study showed that a landlocked population of lamprey was not established. The attacks on fish occurred for only a brief period each year. Attacks on trout stopped approximately 7 yr after dam construction. Because this was the average estimated age when ammocoetes metamorphosed and scarred fish or ammocoetes were no longer found, we concluded that metamorphosed lamprey were unable to survive to maturity. The inability to establish a landlocked population indicated that the transition from an anadromous parasitic life history type to a freshwater parasitic life history type did not occur as quickly or as easily as previously suggested. The study also provided evidence that the statolith method of age determination may provide accurate ages.

scholarly journals Molecular systematics of the Sicista tianschanica species complex: a contribution from historical DNA analysis

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10759
Author(s):  
Vladimir S. Lebedev ◽  
Yulia Kovalskaya ◽  
Evgeniya N. Solovyeva ◽  
Elena D. Zemlemerova ◽  
Anna A. Bannikova ◽  
...  
Keyword(s):  
Species Complex ◽  
Dna Analysis ◽  
Taxonomic Status ◽  
Nuclear Data ◽  
Distinct Species ◽  
Museum Collections ◽  
Central Asian ◽  
The Status ◽  
Northern Tianshan ◽  
Historical Dna

The Tianshan birch mouse Sicista tianschanica is an endemic of the Central Asian mountains and has previously been shown to include several karyomorphs (“Terskey”, “Talgar”, “Dzungar”); however, the taxonomic status of these forms has remained uncertain. We examined the genetic variation in S. tianschanica based on historical DNA samples from museum collections, including the type series. Mitochondrial and nuclear data indicated that the species complex includes two major clades: Northern (N) and Southern (S) (cytb distance 13%). The N clade corresponds to the “Dzungar” karyomorph (Dzungar Alatau, Tarbagatay). The S clade is comprised of four lineages (S1–S4) divergent at 6–8%; the relationships among which are resolved incompletely. The S1 lineage is found in eastern Tianshan and corresponds to the nominal taxon. The S2 is distributed in central and northern Tianshan and corresponds to the “Terskey” karyomorph. The S3 is restricted to Trans-Ili Alatau and belongs to the “Talgar” karyomorph. The S4 is represented by a single specimen from southeastern Dzungar Alatau with "Talgar" karyotype. No interlineage gene flow was revealed. The validity of S. zhetysuica (equivalent to the N clade) is supported. Based on genetic and karyotypic evidence, lineages S2 and S3 are described as distinct species. The status of the S4 requires further investigation.

scholarly journals Phylogeographic Investigations of the Widespread, Arid-Adapted AntlionBrachynemurus sackeniHagen (Neuroptera: Myrmeleontidae)

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Joseph S. Wilson ◽  
Kevin A. Williams ◽  
Clayton F. Gunnell ◽  
James P. Pitts
Keyword(s):  
Genetic Differentiation ◽  
Species Complex ◽  
Evolutionary History ◽  
Mitochondrial Gene ◽  
Distinct Species ◽  
Mountain Building ◽  
Phylogeographic Pattern ◽  
Late Neogene ◽  
Cryptic Species Complex ◽  
Divergence Dates

Several recent studies investigating patterns of diversification in widespread desert-adapted vertebrates have associated major periods of genetic differentiation to late Neogene mountain-building events; yet few projects have addressed these patterns in widespread invertebrates. We examine phylogeographic patterns in the widespread antlion speciesBrachynemurus sackeniHagen (Neuroptera: Myrmeleontidae) using a region of the mitochondrial gene cytochrome oxidase I (COI). We then use a molecular clock to estimate divergence dates for the major lineages. Our analyses resulted in a phylogeny that shows two distinct lineages, both of which are likely distinct species. This reveals the first cryptic species-complex in Myrmeleontidae. The genetic split between lineages dates to about 3.8–4.7 million years ago and may be associated with Neogene mountain building. The phylogeographic pattern does not match patterns found in other taxa. Future analyses within this species-complex may uncover a unique evolutionary history in this group.

Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

2019 ◽  
Vol 28 (2) ◽  
pp. 305-316
Author(s):  
M.A. Chursina ◽  
I.Ya. Grichanov
Keyword(s):  
Dna Analysis ◽  
Distinct Species ◽  
Molecular Data ◽  
Wing Shape ◽  
Morphological Data ◽  
12S Rrna ◽  
Separate Species ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis ◽  
Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

scholarly journals Comparative genomics suggests a taxonomic revision of the Staphylococcus cohnii species complex

2021 ◽  
Author(s):  
Anna Lavecchia ◽  
Matteo Chiara ◽  
Caterina De Virgilio ◽  
Caterina Manzari ◽  
Carlo Pazzani ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Species Complex ◽  
Large Scale ◽  
Genomic Sequence ◽  
Bacterial Species ◽  
Taxonomic Revision ◽  
Distinct Species ◽  
The Novel ◽  
Staphylococcus Cohnii ◽  
Taxonomic Assignments

Abstract Staphylococcus cohnii (SC), a coagulase-negative bacterium, was first isolated in 1975 from human skin. Early phenotypic analyses led to the delineation of two subspecies (subsp.), Staphylococcus cohnii subsp. cohnii (SCC) and Staphylococcus cohnii subsp. urealyticus (SCU). SCC was considered to be specific to humans whereas SCU apparently demonstrated a wider host range, from lower primates to humans. The type strains ATCC 29974 and ATCC 49330 have been designated for SCC and SCU, respectively. Comparative analysis of 66 complete genome sequences—including a novel SC isolate—revealed unexpected patterns within the SC complex, both in terms of genomic sequence identity and gene content, highlighting the presence of 3 phylogenetically distinct groups. Based on our observations, and on the current guidelines for taxonomic classification for bacterial species, we propose a revision of the SC species complex. We suggest that SCC and SCU should be regarded as two distinct species: SC and SU (Staphylococcus urealyticus), and that two distinct subspecies, SCC and SCB (SC subsp. barensis, represented by the novel strain isolated in Bari) should be recognized within SC. Furthermore, since large scale comparative genomics studies recurrently suggest inconsistencies or conflicts in taxonomic assignments of bacterial species, we believe that the approach proposed here might be considered for more general application.

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