scholarly journals Observation by microsatellite DNA analysis of sperm usage in naturally mated honeybee queens (Apis mellifera ligustica) over a period of two years

2008 ◽  
Vol 7 (4) ◽  
pp. 465-478
Author(s):  
Cristina Previtali ◽  
Graziella Bongioni ◽  
Cecilia Costa ◽  
Marco Lodesani ◽  
Andrea Galli
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Dna ◽  
Dna Analysis ◽  
Apis Mellifera Ligustica

scholarly journals Genetic characterization of Italian honeybees,Apis mellifera ligustica, based on microsatellite DNA polymorphisms

Apidologie ◽  
2007 ◽  
Vol 38 (2) ◽  
pp. 207-217 ◽  
Author(s):  
Raffaele Dall'Olio ◽  
Alberto Marino ◽  
Marco Lodesani ◽  
Robin F.A Moritz
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Dna ◽  
Genetic Characterization ◽  
Dna Polymorphisms ◽  
Apis Mellifera Ligustica

scholarly journals Microsatellite DNA polymorphism in selectively controlled Apis mellifera carnica and Apis mellifera caucasica populations from Poland

2015 ◽  
Vol 67 (3) ◽  
pp. 889-894 ◽  
Author(s):  
Stanimila Nikolova ◽  
Malgorzata Bienkowska ◽  
Dariusz Gerula ◽  
Evgeniya Ivanova
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Dna Polymorphism ◽  
Dna Analysis ◽  
Apis Mellifera Carnica ◽  
Expected Heterozygosity ◽  
Apis Mellifera Caucasica ◽  
The Mean ◽  
Private Alleles

Genetic polymorphism in selectively controlled honeybee populations of A. m. carnica and A. m. caucasica in Poland, was characterized by microsatellite DNA analysis. All honeybee samples were analyzed for nine microsatellite loci: Ac011; A024; A043; A088; Ap226; Ap238; Ap243; Ap249 and Ap256, which were found to be polymorphic in both populations. The mean number of alleles per locus was 6.222 for A. m. carnica and 4.556 for A. m. caucasica. Average observed and expected heterozygosity values were calculated as 0.976 and 0.734 in A. m. carnica and as 0.933 and 0.603 in A. m. caucasica, respectively. For the nine microsatellite loci, a total of 76 alleles were found in both populations. Thirty-five private alleles were observed in A. m. carnica and 20 in A. m. caucasica. Information about allele frequencies, FST values and genotypic differentiation is given. Nei?s genetic distance between studied populations of A. m. carnica and A. m. caucasica was calculated as 0.384.

LACK OF WORKER POLICING IN THE CAPE HONEYBEE (APIS MELLIFERA CAPENSIS)

Behaviour ◽  
1999 ◽  
Vol 136 (9) ◽  
pp. 1079-1092 ◽  
Author(s):  
◽  
M.H. Allsopp ◽  
R.F.A. Moritz
Keyword(s):  
Apis Mellifera ◽  
Evolutionary Theory ◽  
Microsatellite Dna ◽  
Dna Analysis ◽  
Worker Reproduction ◽  
Genetic Evidence ◽  
Apis Mellifera Capensis ◽  
Worker Policing ◽  
Cape Honeybee ◽  
Average Worker

AbstractIn honeybees (Apis mellifera) laying worker offspring is rare. One mechanism to suppress worker reproduction is through worker policing, i.e. workers remove unfertilised eggs laid by other workers. This behaviour has been shown to be adaptive as soon as the queen performs polyandrous matings. The average relatedness to the queen's drones is higher than to the worker laid offspring. In the Cape honeybee (A. m. capensis) reproductive workers lay fertilised eggs which develop into females. In this case the average worker relatedness to sexual reproductives reared from worker or queen offspring is identical. Worker policing has been predicted by evolutionary theory to be less expressed in A. m. capensis colonies than in other honeybees. We found genetic evidence that worker policing is not common in the Cape honeybee. Laying worker offspring was identified in queen right colonies using microsatellite DNA analysis.

scholarly journals Microsatellite DNA analysis shows that greater sage grouse leks are not kin groups

2005 ◽  
Vol 14 (14) ◽  
pp. 4453-4459 ◽  
Author(s):  
ROBERT M. GIBSON ◽  
DEBRA PIRES ◽  
KATHLEEN S. DELANEY ◽  
ROBERT K. WAYNE
Keyword(s):  
Microsatellite Dna ◽  
Dna Analysis ◽  
Sage Grouse

The dietary arachidonic acid improved growth and immunity of honey bee (Apis mellifera ligustica)

2021 ◽  
pp. 1-10
Author(s):  
Jing Yu ◽  
Weixing Zhang ◽  
Xuepeng Chi ◽  
Wenfeng Chen ◽  
Zhenfang Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Arachidonic Acid ◽  
Apis Mellifera ◽  
The Body ◽  
Control Group ◽  
Immune Functions ◽  
Pufa Content ◽  
Apis Mellifera Ligustica ◽  
Immune Genes ◽  
Mrna Expression Levels

Abstract Honeybees cannot synthesize arachidonic acid (ARA) themselves, only obtain it from food. Most pollen is deficient or contains a small amount of ARA. The necessity of supplementary ARA in bees’ diet has not been studied. The objective of this study was to investigate the effects of dietary ARA levels on the growth and immunity of Apis mellifera ligustica. A total of 25 honeybee colonies were randomly assigned to five dietary groups which were fed basic diets supplemented with 0, 2, 4, 6, and 8% of ARA. The diet with 4% ARA improved the body weight of newly emerged worker bees compared with the control group. Supplement of ARA in honeybee diets changed the fatty acid composition of honeybee body. SFA and MUFA contents of bees’ body declined, and PUFA content rised in the ARA group. Compared with the control group, the supplement of ARA in honeybee diets increased the contents of ARA, C22:6n-3 (DHA) and C18:3n-6 in bees’ body significantly, but decreased the contents of C16:1 and C18:3n-3. The diet supplied with 4% ARA reduced the mortality rate of honeybee infected with Escherichia coli. The activity of immune enzymes (phenoloxidase, antitrypsin, and lysozyme) and the mRNA expression levels of immune genes (defensin-2, toll, myd88, and dorsal) were improved by ARA diets to varying degrees depending on the ARA levels, especially 4% ARA. These results suggested that dietary ARA could improve the growth, survival, and immune functions of honeybees. Supplement of ARA in bees’ diet would be valuable for the fitness of honeybees.

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