scholarly journals The Effect of ginger essential oil (Zingiber Officinale) as repellent towards Aedes aegypti

2016 ◽  
Vol 32 (10) ◽  
pp. 353
Author(s):  
Ery Agus Mardiansyah ◽  
Sitti Rahmah Umniyati ◽  
Susi Iravati
Keyword(s):  
Experimental Study ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Active Ingredient ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Ability Test ◽  
Protective Ability

The effect of ginger essential oil (Zingiber officinale) as an effective repellent  against the Aedes aegypti mosquitoPurposeThe purpose of this paper was to determine the ability of ginger essential oil (Zingiber officinale) as a repellent towards Aedes aegypti. MethodsThis research was a true experimental study. The tests performed in this study were negative control tests, with protective ability test of telon plus oil and ginger essential oil 4% in telon oil involving 25 Aedes aegypti adult female mosquitoes. ResultsThe study found that the average protective ability of ginger essential oil 4 % in telon oil was 100% at minute 5, 10, and 15, while telon oil plus (positive control) was 5%, 36%, and 33%. In the protective ability test of ginger essential oil 4% in telon oil has the better repellent ability or protective ability compared to telon plus, which can provide protective ability up to 60 minutes at 91.95% while telon plus oil provides protective ability around 59.51%. In the length of protection test ginger essential oil 4% in telon oil gave an average of protection from biting until 61.67 minutes, while telon oil gives 8.33 minutes and 11.67 minutes of telon plus oil. Based on Probit analysis ginger essential oil 4% in telon oil can repel 90% of experimental mosquitoes up to 89.87 minutes. ConclusionGinger essential oil 4% in telon oil has the better repellent ability and length of protection compared to telon plus oil. Ginger essential oil 4% in telon can be used as a repellent active ingredient.

UJI EFEKTIVITAS ANTHELMINTIK EKSTRAK RIMPANG BANGLE (Zingiber purpureum Roxb.) TERHADAP CACING Ascaridia galli SECARA IN VITRO

2017 ◽  
Vol 1 (2) ◽  
pp. 187
Author(s):  
Yanthi Susanti ◽  
Indri Astuti ◽  
Ade Ari Dwi Astuti
Keyword(s):  
Experimental Study ◽  
Data Analysis ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Ascaridia Galli ◽  
Experimental Animals ◽  
Anthelmintic Efficacy ◽  
Pyrantel Pamoate

The research was conducted to observe anthelmintic efficacy on rhizome extract bangle against Ascaridia galli by in vitro procedure. Experimental study was conducted, with samples rhizomes bangle powder and  maceration method in three concentrations of 2%, 4% and 8%. The experimental animals used is Ascaridia galli with NaCl 0.9% as negative control and a pyrantel pamoate suspension as positive control with concentrations of 0.2%, 0.4% and 0.6%. The study was conducted in July 2014 at the Laboratory on IKIFA Academy of Pharmacy. The data analysis used is a probit analysis. Results of Research was rhizome extract bangle (Zingiber purpureum Roxb.) having as anthelmintic efficacy against Ascaridia galli with LC50 2.29% and LT50 in 4 hours 3 minutes 18 seconds

scholarly journals The Effect of Zingiber officinale Roscoe (Ginger) on Dentin Microhardness: An in vitro Study

2017 ◽  
Vol 9 (13) ◽  
pp. 102 ◽  
Author(s):  
Siti Khadijah Mohd Zaki ◽  
Safura Anita Baharin ◽  
Jasmina Qamaruz Zaman ◽  
Hsu Zenn Yew ◽  
Shahida Mohd Said
Keyword(s):  
Essential Oil ◽  
Ethylenediaminetetraacetic Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Human Teeth ◽  
Root Canal Irrigants ◽  
Zingiber Officinale Roscoe ◽  
Root Dentin

It has been established that conventional root canal irrigants cause detrimental effect on the physico-chemical properties of root dentin. This study aims to determine the effect of a novel root irrigant, ginger or Zingiber officinale Roscoe essential oil on root dentin microhardness. Eighty root halves of extracted human teeth were used and prepared by embedding them individually in autopolymerizing acrylic resin. These samples were then divided into four groups (n = 20 each), i.e., i) 0.5% Z. officinale Roscoe oil, ii) 2.5% sodium hypochlorite (NaOCl, positive control), iii) 17% ethylenediaminetetraacetic acid (EDTA, positive control) and iv) normal saline (negative control). Dentin microhardness was measured using Vickers Microhardness Tester with a 50 g load and a 10-second dwell time, before and after immersion in respective solutions for 5 minutes. Measurements were taken in Vickers hardness number (VHN). All samples showed reduction in the microhardness reading; with Z. officinale Roscoe: 34.24 to 25.89, NaOCl: 31.47 to 26.34, EDTA: 33.08 to 26.04 and saline: 29.82 to 27.61 VHN. Although there was significant reduction within the Z. officinale Roscoe group (p < 0.001), the change was indifferent when compared among groups (post hoc Tukey’s HSD test p > 0.05). Within the limitations of this study, Zingiber officinale Roscoe essential oil does alter dentin microhardness but to a similar extent as NaOCl and EDTA.

scholarly journals UJI VARIASI DOSIS PERASAN LENGKUAS (Alpinia galanga) TERHADAP PERTUMBUHAN KUMAN Staphylococcus aureus

2017 ◽  
Vol 1 (1) ◽  
pp. 23
Author(s):  
Evy Ratnasari Ekawati ◽  
Prasetyo Handriyanto
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Essential Oil ◽  
Growth Inhibition ◽  
Positive Control ◽  
Statistical Test ◽  
Negative Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Infections caused by Staphylococcus aureus if not handled effectively will be able to cause death human. Hasn’t been found effective antibacterial against Staphylococcus aureus. Antibacterial active compounds in the essential oil of ginger have the ability as an antimicrobial, so it can be used as an antibacterial. This study aims to determine effectiveness of the juice of galangal (Alpina galanga) to the growth of Staphylococcus aureus. This study was an experimental study using disc diffusion method. The juice galangal (Alpina galanga) used has dosage of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. At dosage of 50% to 100% has the potential to inhibit the growth Staphylococcus aureus. Nonetheless, based on the statistical test dosage of 50%, 60%, 70%, and 80% was significantly different from dosage 90%, 100%, and positive control and negative control. Dosage 90% and 100% was not significantly different, making more used dosage 90% to accelerate the process of growth inhibition Staphylococcus aureus.

scholarly journals Toxicity Study of Volatile Oil Boesenbergia pandurata (Roxb) Schlecht Rhizome to Larvacide of Aedes aegypti and GC-MS Profile

2018 ◽  
Vol 23 (2) ◽  
pp. 97
Author(s):  
Widyasari Putranti ◽  
Saiful Bachri
Keyword(s):  
Aedes Aegypti ◽  
Aqueous Ethanol ◽  
Volatile Oil ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Active Constituent ◽  
Methyl Cinnamate ◽  
Traditional Drug ◽  
Long Time

Boesenbergia pandurata (Roxb) Schlecht has been recognized by society as traditional drug for a long time. The active constituent of this plants especially the volatile oil, which contains monoterpenes and sesquiterpenes. The use of Boesenbergia pandurata (Roxb) Schlecht rhizome which is a botanical insecticide shows higher safety because the molecule is easily broken down into harmless compounds against the environment. The aim of this research were to know about the activity of larvacide from the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizome and also to analyze its chemical compounds using GC-MS. The volatile oil was isolated from Boesenbergia pandurata (Roxb) Schlecht with steam and water destillation method. The volatile oil in the aqueous ethanol 70 % v/v was made as the test solution for larvacide. The concentrations of the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizomes used were 150 ppm; 201 ppm; 270 ppm; 362 ppm; 485 ppm; 650 ppm. Abate was used as positive control at concentration 0,001 ppm; 0,0025 ppm; 0,0065 ppm; 0,016 ppm; 0,04 ppm; 0,1 ppm while aqueous ethanol 70 % v/v was used as negative control. Each treatment were used 20 larvaes of Aedes aegypti and was observed during 24 hours. The data of died larvae were counted to estimate the values of LC50 with probit analysis method. The chemical compound of volatile oil of Boesenbergia pandurata (Roxb) Schlecht was analyzed by the GC-MS. The results of this research showed that the volatile oil of Boesenbergia pandurata (Roxb) Schlecht have clear-brass colour, bitter taste, typically aromatic, rendemen equal to (0,25 ± 0,011) % v/w and refractive index 1,4818. The value of LC50 for the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizome is (407,06 ± 31,6985) ppm while abate equal to (0,0132 ± 0,0013) ppm. This matter indicates that abate more potent to larvae of Aedes aegypti. The result of analyze the component of the volatile oil of Boesenbergia pandurata (Roxb) Schlecht by the GC-MS obtained of 15 peak chromatogram and 6 peak which has identified showed the possibility the existence of champene, eucalyptol, ocimene, camphora, geraniol, methyl cinnamate.

scholarly journals Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

2018 ◽  
Vol 66 (4) ◽  
pp. 1519
Author(s):  
Mailen Ortega Cuadros ◽  
Adriana Patricia Tofiño Rivera ◽  
Luciano Jose Merini ◽  
Maria Cecilia Martinez Pabon
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Human Fibroblasts ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals MORPHOLOGICAL AND HISTOLOGICAL EFFECTS OF BRUCEINE A ON THE LARVAE OF AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE)

2018 ◽  
Vol 11 (10) ◽  
pp. 422
Author(s):  
Dwi Sutiningsih ◽  
Mustofa Mustofa ◽  
Tri Baskoro Tunggul Satoto ◽  
Edhi Martono
Keyword(s):  
Epithelial Cells ◽  
Aedes Aegypti ◽  
Histological Change ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Histological Changes ◽  
Brucea Javanica ◽  
Gastrointestinal Damage

Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.

scholarly journals Insecticidal and Biting Deterrent Activities of Magnolia grandiflora Essential Oils and Selected Pure Compounds against Aedes aegypti

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1359
Author(s):  
Abbas Ali ◽  
Nurhayat Tabanca ◽  
Betul Demirci ◽  
Vijayasankar Raman ◽  
Jane M. Budel ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Screening Program ◽  
Negative Control ◽  
Magnolia Grandiflora ◽  
Plant Parts ◽  
Pure Compounds ◽  
Index Value

In our natural products screening program for mosquitoes, we tested essential oils extracted from different plant parts of Magnolia grandiflora L. for their insecticidal and biting deterrent activities against Aedes aegypti. Biting deterrence of seeds essential oil with biting deterrence index value of 0.89 was similar to N,N-diethyl-3-methylbenzamide (DEET). All the other oils were active above the solvent control but the activity was significantly lower than DEET. Based on GC-MS analysis, three pure compounds that were only present in the essential oil of seed were further investigated to identify the compounds responsible for biting deterrent activity. 1-Decanol with PNB value of 0.8 was similar to DEET (PNB = 0.8), whereas 1-octanol with PNB value of 0.64 showed biting deterrence lower than 1-decanol and DEET. The activity of 1-heptanol with PNB value of 0.36 was similar to the negative control. Since 1-decanol, which was 3.3% of the seed essential oil, showed biting deterrence similar to DEET as a pure compound, this compound might be responsible for the activity of this oil. In in vitro A & K bioassay, 1-decanol with MED value of 6.25 showed higher repellency than DEET (MED = 12.5). Essential oils of immature and mature fruit showed high toxicity whereas leaf, flower, and seeds essential oils gave only 20%, 0%, and 50% mortality, respectively, at the highest dose of 125 ppm. 1-Decanol with LC50 of 4.8 ppm was the most toxic compound.

scholarly journals Yumurtacı Tavuk Yemlerine Biberiye (Rosmarinus officinalis L.) Uçucu Yağ İlavesinin Performans, Yumurta Kalite Kriterleri ve Yumurta Sarısı Lipit Oksidasyonu Üzerine Etkisi

2016 ◽  
Vol 4 (2) ◽  
pp. 113 ◽  
Author(s):  
Tülay Çimrin ◽  
Murat Demirel
Keyword(s):  
Vitamin E ◽  
Essential Oil ◽  
Storage Time ◽  
Egg Quality ◽  
Egg Yolk ◽  
Positive Control ◽  
Negative Control ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Rosemary Essential Oil

This experiment was designed to investigate the effects of rosemary essential oils in various doses on hen performance, egg quality, lipid peroxidation malondialdehyde (MDA) level in fresh and stored egg yolk. The experiment was carried out in random parcel experimental design. In the experiment, 6 groups were formed and each group was divided into 5 replicates. In total 240 Bovans hite laying hens, each replicate included 8 hens, 32 weeks old, were used. Treatment groups were control (Negative Control), 500 mg//kg antibiotic (Positive Control 1), 200 mg/kg vitamin E (Positive Control 2), 100 mg/kg rosemary essential oil, 200 mg/kg rosemary essential oil, 300 mg/kg rosemary essential oil. There was no significant difference in live weight, egg production, egg weight, damaged egg ratio, mortality and egg quality but feed comsumption, feed conversion ratio (FCR) and egg yolk MDA values were affected significantly in between control and treatment groups. At all treatment groups feed comsumption decreased significantly and other groups improved in feed conversion ratio except 500 mg/kg antibiotic and 100 mg/kg rosemary doses groups compared to negative control significantly. 100 mg/kg rosemary essential oil addition during the storage time for 42 and 56 days and 200 mg/kg vitamin E addition for all storage time to ration decreased egg yolk MDA values significantly. As a result, laying birds fed 200 mg/kg vitamin E, and 100 mg/kg of rosemary essential oil additives can use to prevent lipid oxidation.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

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