scholarly journals The Effect of Zingiber officinale Roscoe (Ginger) on Dentin Microhardness: An in vitro Study

2017 ◽  
Vol 9 (13) ◽  
pp. 102 ◽  
Author(s):  
Siti Khadijah Mohd Zaki ◽  
Safura Anita Baharin ◽  
Jasmina Qamaruz Zaman ◽  
Hsu Zenn Yew ◽  
Shahida Mohd Said
Keyword(s):  
Essential Oil ◽  
Ethylenediaminetetraacetic Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Human Teeth ◽  
Root Canal Irrigants ◽  
Zingiber Officinale Roscoe ◽  
Root Dentin

It has been established that conventional root canal irrigants cause detrimental effect on the physico-chemical properties of root dentin. This study aims to determine the effect of a novel root irrigant, ginger or Zingiber officinale Roscoe essential oil on root dentin microhardness. Eighty root halves of extracted human teeth were used and prepared by embedding them individually in autopolymerizing acrylic resin. These samples were then divided into four groups (n = 20 each), i.e., i) 0.5% Z. officinale Roscoe oil, ii) 2.5% sodium hypochlorite (NaOCl, positive control), iii) 17% ethylenediaminetetraacetic acid (EDTA, positive control) and iv) normal saline (negative control). Dentin microhardness was measured using Vickers Microhardness Tester with a 50 g load and a 10-second dwell time, before and after immersion in respective solutions for 5 minutes. Measurements were taken in Vickers hardness number (VHN). All samples showed reduction in the microhardness reading; with Z. officinale Roscoe: 34.24 to 25.89, NaOCl: 31.47 to 26.34, EDTA: 33.08 to 26.04 and saline: 29.82 to 27.61 VHN. Although there was significant reduction within the Z. officinale Roscoe group (p < 0.001), the change was indifferent when compared among groups (post hoc Tukey’s HSD test p > 0.05). Within the limitations of this study, Zingiber officinale Roscoe essential oil does alter dentin microhardness but to a similar extent as NaOCl and EDTA.

scholarly journals Control of Alternaria alternata Using Melaleuca Essential Oil (Melaleuca alternifolia)

2019 ◽  
pp. 1-10
Author(s):  
Flávia Mota de Figuerêdo Alves ◽  
Kevison Romulo da Silva França ◽  
Ionaly Gomes de Araújo ◽  
Lídia Pinheiro da Nóbrega ◽  
Alda Leaby dos Santos Xavier ◽  
...  
Keyword(s):  
Essential Oil ◽  
Alternaria Alternata ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Positive Control ◽  
Negative Control ◽  
Growth Speed ◽  
In Vitro Test

Aims: This study aimed to evaluate the fungitoxic potential of melaleuca essential oil on the mycelial growth of Alternaria alternata under in vitro condition and the treatment of cowpea beans. Study Design: The experiments comprised completely randomized designs: Eleven treatments with five replicates on in vitro test; and six treatments with five replicates on in vivo test. Place and Duration of Study: The work was carried out at the Center for Agrifood Science and Technology of the Federal University of Campina Grande, Pombal, Brazil, since February 2018 to February 2019. Methodology: In the in vitro experiment, the essential oil was incorporated into the culture medium and poured into Petri dishes. The treatments consisted of different concentrations of the essential oil (0.0125, 0.025, 0.05, 0.1, 0.2, 0.25, 0.50, 0.75, and 1.0%), a negative control (0.0%), and a positive control (Thiram). Discs of culture medium with fungal mycelia were inoculated in the center of the plates and incubated for seven days at 27±2ºC. The percentage of mycelial growth inhibition (PGI) and the index of mycelial growth speed (IMGS) was calculated to verify the difference between treatments. In the in vivo experiment, the bean seeds were treated with different concentrations of EO (0.0, 0.2, 0.5, 1.0, and 5.0%), a negative control (0.0%), and positive control (Thiram). Seeds were inoculated with colonies of the fungus for 48 hours, and after that, we performed the seed sanity test. Results: Under in vitro conditions, all concentrations of melaleuca essential oil reduced the mycelial growth of A. alternata. The oil reached complete inhibition of fungal growth from 0.2% concentration and above. In the cowpea treatment, the essential oil had no significant control over the percentage of infected seeds. Conclusion: The melaleuca essential oil had a fungitoxic effect on the A. alternata under in vitro conditions. However, using the adopted methodology, on the cowpea bean seed treatment, the essential oil did not reduce the incidence of A. alternata.

scholarly journals Effect of exposure to etidronic acid on the bond strength of calcium silicate-based cements after 1 and 21 days: an in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Elena Rebolloso de Barrio ◽  
Juan José Pérez-Higueras ◽  
Ernesto García-Barbero ◽  
Lucía Gancedo-Caravia
Keyword(s):  
Bond Strength ◽  
Root Canal ◽  
Calcium Silicate ◽  
Ethylenediaminetetraacetic Acid ◽  
Setting Time ◽  
Etidronic Acid ◽  
Human Teeth ◽  
Root Canal Irrigants ◽  
Push Out

Abstract Background After reparation of root perforations with calcium silicate-based cements (CSBC), the surface of the material is expected to be exposed to root canal irrigants (RCI) while resuming the root canal treatment. Methods The aim of this study was to compare the effect of exposure to a mixture of sodium hypochlorite (NaOCl) and etidronic acid (HEBP) or other irrigants on the Push Out Bond Strength (POBS) of CSBC after two different setting times. 240 root slices 1 mm thick were obtained from single-rooted human teeth. A 1.4 mm diameter perforation was performed on each slice and filled with Biodentine (BD) or ProRoot MTA (PMTA). After 1 or 21 days they were exposed to 17% ethylenediaminetetraacetic acid, 5.25% NaOCl, a mixture of 5.25% NaOCl and 9% HEBP (NaOCl + HEBP) or saline (n = 15) and submitted to a push-out test. POBS results were analysed with ANOVA and Tukey tests. Results BD showed higher POBS than PMTA after 1 day (p < .05). After 21 days no differences were found between materials. After 1 day exposure to NaOCl + HEBP resulted in higher POBS, compared to the other irrigants (p < .05). Conclusion POBS results are influenced by the cement, the setting time and the exposure to irrigants.

scholarly journals Combination of Propolis Extract (Trigona Sp) and Ginger Rhizome (Zingiber Officinale Roscoe) as Alternative Antibiotics against Escherichia Coli In Vitro

2019 ◽  
Vol 6 (3) ◽  
pp. 112
Author(s):  
Abdul Wahid Jamaluddin ◽  
Muhammad F. Mursalim ◽  
Andi M. S. Apada
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Inhibitory Activity ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Ginger Extract ◽  
Propolis Extract ◽  
Escherichia Coli Bacteria

Escherichia coli is a gram negative bacterium which is a normal flora in the digestive tract. In birds, this bacterium causes a disease known as colibasillosis. Antibiotics are generally used, but excessive use will cause residues and antibiotic resistance. To avoid resistance or residue, an alternative treatment is needed. The combination of propolis and ginger extract is very promising to develop because both have a synergistic effect as antimicrobials. The research aims to determine the effect of the combination extract on Escherichia coli bacteria in vitro. We used 8 groups which contain combinations of propolis and ginger extract. We used amoxicillin disk as a positive control, 1% Na CMC as a negative control. The results showed a combination of ginger and propolis extract showed good inhibitory activity against Escherichia coli in all groups> 6mm., and the highest inhibitory activity was K3 (5% propolis combination and 15% ginger extract) with 8.7 mm. The combination of propolis and ginger extract has the potential to be used as an alternative antibiotic to prevent antibiotic resistance from synthetic antibiotics.Keywords: alternative antibiotics, combination extracts, Escherichia coli, ginger, in vitro, propolis

scholarly journals In vitro study of dentin hypersensitivity treated by whitlockite glass-ceramics

2017 ◽  
Author(s):  
Amanda C. Juraski
Keyword(s):  
Bioactive Glass ◽  
Artificial Saliva ◽  
Third Molar ◽  
Glass Ceramics ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Dentin Hypersensitivity ◽  
Human Teeth

AbstractThe aim of this study is to evaluate the potential of a bioactive glass based on the 3CaO.P2O5-SiO2MgO-system and its glassceramics containing whitlockite on the remineralization of dentin as a possible treatment to dentin hypersensitivity. For that, 40 third molar human teeth were artificially demineralized and randomly distributed in 4 groups (n = 10): G1 - Negative Control (no treatment), G2 - Positive Control (treated by Bioglass® 45S5), G3 – BG (treated by bioactive glass based on 3CaO.P2O5-SiO2-MgOsystem), and G4 – BGC (treated by bioactive whitlockite glass-ceramics). After treatment, the samples were emerged in artificial saliva and stored for 7 days in a controlled temperature of 37ºC. After that, scanning electron microscopy (SEM) and atomic force microscopy (AFM) was used to evaluate samples morphology. The analysis confirmed the formation of hydroxyapatite on the surface of all the biomaterials studied, that in the dentine specimens treated by bioactive glass and whitlockite glas-ceramic most of the dentinal tubules were completely occluded.

scholarly journals The Effect of ginger essential oil (Zingiber Officinale) as repellent towards Aedes aegypti

2016 ◽  
Vol 32 (10) ◽  
pp. 353
Author(s):  
Ery Agus Mardiansyah ◽  
Sitti Rahmah Umniyati ◽  
Susi Iravati
Keyword(s):  
Experimental Study ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Active Ingredient ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Ability Test ◽  
Protective Ability

The effect of ginger essential oil (Zingiber officinale) as an effective repellent  against the Aedes aegypti mosquitoPurposeThe purpose of this paper was to determine the ability of ginger essential oil (Zingiber officinale) as a repellent towards Aedes aegypti. MethodsThis research was a true experimental study. The tests performed in this study were negative control tests, with protective ability test of telon plus oil and ginger essential oil 4% in telon oil involving 25 Aedes aegypti adult female mosquitoes. ResultsThe study found that the average protective ability of ginger essential oil 4 % in telon oil was 100% at minute 5, 10, and 15, while telon oil plus (positive control) was 5%, 36%, and 33%. In the protective ability test of ginger essential oil 4% in telon oil has the better repellent ability or protective ability compared to telon plus, which can provide protective ability up to 60 minutes at 91.95% while telon plus oil provides protective ability around 59.51%. In the length of protection test ginger essential oil 4% in telon oil gave an average of protection from biting until 61.67 minutes, while telon oil gives 8.33 minutes and 11.67 minutes of telon plus oil. Based on Probit analysis ginger essential oil 4% in telon oil can repel 90% of experimental mosquitoes up to 89.87 minutes. ConclusionGinger essential oil 4% in telon oil has the better repellent ability and length of protection compared to telon plus oil. Ginger essential oil 4% in telon can be used as a repellent active ingredient.

scholarly journals Actividad antifúngica de Cymbopogon citratus contra Colletotrichum gloeosporioides

2017 ◽  
Vol 28 (2) ◽  
pp. 465 ◽  
Author(s):  
Alexander Francisco Pérez Cordero ◽  
Leonardo Miguel Chamorro Anaya ◽  
Deimer Calixto Vitola Romero ◽  
Jesús María Hernández Gómez
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
Colletotrichum Gloeosporioides ◽  
Chemical Characterization ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
A Value

The objective of this research was to evaluate in vitro the inhibitory activity of essential oils from fresh leaves of Cymbopogon citratus (lemongrass) against Colletotrichum gloeosporioides in yam. The research was conducted in the department of Sucre, Colombia. The essential oils of C. citratus were collected in the municipalities of Sincelejo, La Union and Sampues, in September and October 2015. The essential oil was extract from fresh leaves using the microwave-assisted hydrodistillation. Concentrations of 5000, 8000 and 10 000 ppm of each essential oil were prepared. An absolute control, a positive control (benomyl 1 g/l) and a negative control was used. An inhibitory activity was obtained by using the direct seeding on surface of the potato-dextrose-agar method and it was expressed as percentage of inhibition rate. The chemical characterization of essential oils was performed by gas chromatography coupled to mass spectrometry. The highest percentages of antifungal index were observed in the lemongrass from Sincelejo at concentrations of 5000, 8000 and 10 000 ppm, after in La Union at 8000 and 10 000 ppm, and finally at Sampues at 10 000 ppm, with a value of 97.77%; the effect was similar to the positive control with benomyl 1 g/l. Citral was the main constituent of the essential oils extracted. The essential oils obtained from the three municipalities showed antifungal activity against C. gloeosporioides. 

Inhibitory effects of ginger (Zingiber officinale Roscoe) essential oil on leukocyte migration in vivo and in vitro

2010 ◽  
Vol 65 (1) ◽  
pp. 241-246 ◽  
Author(s):  
Gessilda Alcantara Nogueira de Melo ◽  
Renata Grespan ◽  
Jefferson Pitelli Fonseca ◽  
Thiago Oliveira Farinha ◽  
Expedito Leite da Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Zingiber Officinale ◽  
Leukocyte Migration ◽  
Inhibitory Effects ◽  
Zingiber Officinale Roscoe

In Vitro Comparison of MTA and BC RRM-Fast Set Putty as Retrograde Filling Materials

2021 ◽  
Author(s):  
Carla Arvelaiz ◽  
Andreina Fernandes ◽  
Veronica Graterol ◽  
Katiuska Gomez ◽  
Jose Francisco Gomez-Sosa ◽  
...  
Keyword(s):  
Brain Heart Infusion ◽  
Bacterial Count ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Material ◽  
Human Teeth ◽  
Retrograde Filling ◽  
Significant Difference

Abstract Background: To compare, in vitro, the bioceramic materials (MTA and BC RRM-fast set putty) capacity to prevent microleakage of Enterococcus faecalis over time. Methods: An experimental design was made with forty extracted human teeth, coronally cut, and prepared to be placed in a leakage system under sterile conditions. They were randomly divided into an experimental group: thirty teeth (fifteen for retrograde filling material MTA and BC RRM-fast set putty, respectively) and a control group: ten teeth (five positive control, five negative control). The 3 mm root-ends were submerged in a brain-heart infusion broth with a red phenol indicator. The coronal access of each sample was inoculated with E. faecalis every seven days to maintain bacterial viability. The lower chamber was evaluated daily for 30 days to observe the turbidity of the culture medium and establish the presence and day of the filtration. Calculation of the colony-forming units (CFU) was performed for each leaked sample. Results: Of the total samples prepared for each type of bioceramic material, leaked 60.0% (9/15) of the MTA group and 40.0% (6/15) of the BC RRM-fast set putty group. All positive controls filtered on the first day of evaluation, while 20% (1/5) of the negative control leaked in the second week. There was no significant difference in leakage between the bioceramic material types, nor concerning the bacterial count and the type of cement used (p = 0.101). Conclusions: This study suggests that BC RRM-fast set putty and MTA have a similar sealing capacity when used as a retrograde filling material in vitro.

scholarly journals The sensitivity of Demodex canis (Acari: Demodicidae) to the essential oil of Melaleuca alternifolia – an in vitro study

2020 ◽  
Vol 29 (3) ◽  
Author(s):  
Rita de Cássia da Silva Machado Neves ◽  
Luciano Antunes Barros ◽  
Shihane Mohamad Costa Mendes ◽  
Thalita Irineu de Souza Werneck de Assis de Amorim ◽  
Vany Perpétua Ferraz ◽  
...  
Keyword(s):  
Essential Oil ◽  
In Vitro Study ◽  
Lethal Effect ◽  
Positive Control ◽  
Negative Control ◽  
Survival Times ◽  
Melaleuca Alternifolia ◽  
Tea Tree ◽  
Demodex Canis

Abstract The essential oil of the Melaleuca alternifolia (Maiden & Betche) (tea tree oil) has been effective in previous studies, in the treatment of infestation by Demodex mites in humans. The present study aimed at evaluating the in vitro acaricidal effect of this herbal medicine on Demodex canis. For the parasitological examination, samples were collected from a dog’s skin and examined using optical microscopy. Only samples with intact mites and with evident movement of chelicerae and tarsi were selected. Twenty-one samples were tested with the oil, in seven different concentrations: 100%; 50%; 25%; 12.5%; 6.25%; 5.0% and 3.13%. Three samples were tested with the positive control amitraz, and three with the negative control neutral shampoo. The interval between the time the solution was added and the moment the movement of the last mite ceased defined the survival time in the sample. By comparing the times at different concentrations and controls, the results showed that the higher the concentration of the oil, the more quickly its lethal effect occurred, and that the survival times in the controls were longer than in the different concentrations of melaleuca oil.

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