scholarly journals Toxicity Study of Volatile Oil Boesenbergia pandurata (Roxb) Schlecht Rhizome to Larvacide of Aedes aegypti and GC-MS Profile

2018 ◽  
Vol 23 (2) ◽  
pp. 97
Author(s):  
Widyasari Putranti ◽  
Saiful Bachri
Keyword(s):  
Aedes Aegypti ◽  
Aqueous Ethanol ◽  
Volatile Oil ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Active Constituent ◽  
Methyl Cinnamate ◽  
Traditional Drug ◽  
Long Time

Boesenbergia pandurata (Roxb) Schlecht has been recognized by society as traditional drug for a long time. The active constituent of this plants especially the volatile oil, which contains monoterpenes and sesquiterpenes. The use of Boesenbergia pandurata (Roxb) Schlecht rhizome which is a botanical insecticide shows higher safety because the molecule is easily broken down into harmless compounds against the environment. The aim of this research were to know about the activity of larvacide from the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizome and also to analyze its chemical compounds using GC-MS. The volatile oil was isolated from Boesenbergia pandurata (Roxb) Schlecht with steam and water destillation method. The volatile oil in the aqueous ethanol 70 % v/v was made as the test solution for larvacide. The concentrations of the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizomes used were 150 ppm; 201 ppm; 270 ppm; 362 ppm; 485 ppm; 650 ppm. Abate was used as positive control at concentration 0,001 ppm; 0,0025 ppm; 0,0065 ppm; 0,016 ppm; 0,04 ppm; 0,1 ppm while aqueous ethanol 70 % v/v was used as negative control. Each treatment were used 20 larvaes of Aedes aegypti and was observed during 24 hours. The data of died larvae were counted to estimate the values of LC50 with probit analysis method. The chemical compound of volatile oil of Boesenbergia pandurata (Roxb) Schlecht was analyzed by the GC-MS. The results of this research showed that the volatile oil of Boesenbergia pandurata (Roxb) Schlecht have clear-brass colour, bitter taste, typically aromatic, rendemen equal to (0,25 ± 0,011) % v/w and refractive index 1,4818. The value of LC50 for the volatile oil of Boesenbergia pandurata (Roxb) Schlecht rhizome is (407,06 ± 31,6985) ppm while abate equal to (0,0132 ± 0,0013) ppm. This matter indicates that abate more potent to larvae of Aedes aegypti. The result of analyze the component of the volatile oil of Boesenbergia pandurata (Roxb) Schlecht by the GC-MS obtained of 15 peak chromatogram and 6 peak which has identified showed the possibility the existence of champene, eucalyptol, ocimene, camphora, geraniol, methyl cinnamate.

scholarly journals The Effect of ginger essential oil (Zingiber Officinale) as repellent towards Aedes aegypti

2016 ◽  
Vol 32 (10) ◽  
pp. 353
Author(s):  
Ery Agus Mardiansyah ◽  
Sitti Rahmah Umniyati ◽  
Susi Iravati
Keyword(s):  
Experimental Study ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Active Ingredient ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Ability Test ◽  
Protective Ability

The effect of ginger essential oil (Zingiber officinale) as an effective repellent  against the Aedes aegypti mosquitoPurposeThe purpose of this paper was to determine the ability of ginger essential oil (Zingiber officinale) as a repellent towards Aedes aegypti. MethodsThis research was a true experimental study. The tests performed in this study were negative control tests, with protective ability test of telon plus oil and ginger essential oil 4% in telon oil involving 25 Aedes aegypti adult female mosquitoes. ResultsThe study found that the average protective ability of ginger essential oil 4 % in telon oil was 100% at minute 5, 10, and 15, while telon oil plus (positive control) was 5%, 36%, and 33%. In the protective ability test of ginger essential oil 4% in telon oil has the better repellent ability or protective ability compared to telon plus, which can provide protective ability up to 60 minutes at 91.95% while telon plus oil provides protective ability around 59.51%. In the length of protection test ginger essential oil 4% in telon oil gave an average of protection from biting until 61.67 minutes, while telon oil gives 8.33 minutes and 11.67 minutes of telon plus oil. Based on Probit analysis ginger essential oil 4% in telon oil can repel 90% of experimental mosquitoes up to 89.87 minutes. ConclusionGinger essential oil 4% in telon oil has the better repellent ability and length of protection compared to telon plus oil. Ginger essential oil 4% in telon can be used as a repellent active ingredient.

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals MORPHOLOGICAL AND HISTOLOGICAL EFFECTS OF BRUCEINE A ON THE LARVAE OF AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE)

2018 ◽  
Vol 11 (10) ◽  
pp. 422
Author(s):  
Dwi Sutiningsih ◽  
Mustofa Mustofa ◽  
Tri Baskoro Tunggul Satoto ◽  
Edhi Martono
Keyword(s):  
Epithelial Cells ◽  
Aedes Aegypti ◽  
Histological Change ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Histological Changes ◽  
Brucea Javanica ◽  
Gastrointestinal Damage

Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

scholarly journals AKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI LARVASIDA TERHADAP Aedes aegypti DI KECAMATAN KELAPA LIMA KOTA KUPANG

2020 ◽  
Vol 8 (1) ◽  
pp. 54-68
Author(s):  
Deswandi W. S. Berri ◽  
Julianty Almet ◽  
Diana Agustiani Wuri
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Research Method ◽  
Hemorrhagic Fever ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mosquito Vector ◽  
Curcuma Xanthorrhiza ◽  
Mosquito Vector Control

Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.

scholarly journals EFEKTIVITAS EKSTRAK DAUN JERUK PURUT (Citrus hystrix) SEBAGAI INSEKTISIDA ALAMI TERHADAP KECOAK AMERIKA (Periplaneta americana) DENGAN METODE SEMPROT

2020 ◽  
Vol 8 (1) ◽  
pp. 8-14
Author(s):  
Hanina Hanina ◽  
Sarah Madeleyne Baringbing
Keyword(s):  
Leaf Extract ◽  
Periplaneta Americana ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Observation Time ◽  
American Cockroach ◽  
Control Group ◽  
Significant Difference ◽  
Post Test

ABSTRACT Cockroach control can be done in various ways one of which is spraying insecticide. Natural insecticides are less dangerous than conventional insecticides. This study aimed to determined the effectiveness of kaffir lime leaf extract (Citrus hystrix) as a natural insecticide against cockroach americana (Periplaneta americana) with spray method. This research was an experimental study with a post-test only control group designed design. The sample in this study was 60 cockroaches, the sample was divided into 6 groups: negative control (aquades), positive control (transflutrin 0.06%) and and 4 test groups with concentrations of kaffir lime leaf extract (20%, 40%, 60% , and 80%) each group consisted of 10 cockroaches. Phytochemical tests were carried out to determine the content of secondary metabolites in extracts of kaffir lime leaves. Positive phytochemical test results containing flavonoids, alkaloids and essential oils. Based on probit analysis, it is known that the LC50 value = 58.5%; LC95 = 85.1%. The time of death of LT50 is fastest at a concentration of 60% (3,036 hours) and LT95 is the fastest at a concentration of 80% (5,960 hours). Anova test analysis results showed that the positive control group had no significant difference with kaffir lime leaf extract concentration of 80% Kaffir lime leaf extract has an insecticide effect, the higher the concentration of kaffir lime leaf extract, the more number of dead cockroaches and the longer the observation time, the number of cockroaches death will also increase. Keywords: American cockroach, kaffir lime leaf extract, cockroach death   ABSTRAK Pengendalian kecoa dapat dilakukan dengan berbagai cara salah satunya penyemprotan insektisida. Insektisida alami kurang berbahaya dibanding insektisida konvensional. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun jeruk purut (Citrus hystrix) sebagai insektisida alami terhadap kecoak americana (Periplaneta americana) dengan metode semprot. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan post-test only control group designed. Sampel dalam penelitian ini adalah 60 ekor kecoak, sampel dibagi menjadi 6 kelompok yaitu kontrol negatif (aquades), kontrol positif (transflutrin 0,06%) dan dan 4 kelompok uji dengan konsentrasi ekstrak daun jeruk purut (20%, 40%, 60%, dan 80%) masing-masing kelompok terdiri dari 10 ekor kecoak. Uji fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder pada ekstrak daun jeruk purut. Hasil uji fitokimia positif mengandung flavonoid, alkaloid dan minyak atsiri. Berdasarkan analisis probit, diketahui nilai LC50 = 58,5%; LC95 = 85,1%. Waktu kematian LT50 paling cepat pada konsentrasi 60% (3,036 jam) dan LT95 paling cepat pada konsentrasi 80% (5,960 jam). Hasil analisis uji anova menunjukkan bahwa kelompok kontrol positif tidak terdapat perbedaan yang signifikan dengan ekstrak daun jeruk purut konsentrasi 80% Ekstrak daun jeruk purut memiliki efek insektisida, semakin tinggi konsentrasi ekstrak daun jeruk purut maka jumlah kecoak yang mati semakin banyak dan semakin lama waktu pengamatan, maka jumlah kecoak yang mati juga akan semakin banyak. Kata kunci : kecoak amerika, ekstrak daun jeruk purut, kematian kecoak

scholarly journals AEDES AEGYPTI SURVIVORSHIP ON SALT TOLERANT CALIFORNIA LANDSCAPE PLANTS

2021 ◽  
Vol 66 (1) ◽  
pp. 7-10
Author(s):  
Christopher S. Bibbs
Keyword(s):  
Aedes Aegypti ◽  
Plant Species ◽  
Sucrose Solution ◽  
Water Solution ◽  
The United States ◽  
Positive Control ◽  
Negative Control ◽  
Arid Environments ◽  
Salt Cedar ◽  
Riparian Plants

Aedes aegypti has expanded its range in the United States to include various arid and desert geographies, with notable introduction into various parts of California. Because resources are limited in arid environments, it is currently an important topic to understand how Ae. aegypti interacts with its surrounding environment for survival and proliferation. Three common plant species in peridomestic landscape, i.e., salt cedar (Tamarix aphylla), arrow weed (Pluchea sericea) and four wing saltbush (Atriplex canescens), were collected for survival bioassays to understand how Ae. aegypti is persisting in arid, chaparral landscapes in California, USA. These three plant-species along with a 10% sucrose solution (positive control) and reverse osmosis water solution (negative control-) were added to cages of Ae. aegypti to assess their survival at 24h, 48h, and 96h. It was found, in comparison with the negative control and four wing salt bush, that arrow weed and to a lesser extent salt cedar, promoted survival of Ae. aegypti in the first 24h. After the first day, only arrow weed significantly supported mosquito survival out to 96h as compared to the controls. Arrow weed and salt cedar are both riparian plants producing some nectaries which could be energy resources provided through stem sap or nectar to Ae. aegypti amidst peridomestic chaparral in California.

UJI EFEKTIVITAS ANTHELMINTIK EKSTRAK RIMPANG BANGLE (Zingiber purpureum Roxb.) TERHADAP CACING Ascaridia galli SECARA IN VITRO

2017 ◽  
Vol 1 (2) ◽  
pp. 187
Author(s):  
Yanthi Susanti ◽  
Indri Astuti ◽  
Ade Ari Dwi Astuti
Keyword(s):  
Experimental Study ◽  
Data Analysis ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Ascaridia Galli ◽  
Experimental Animals ◽  
Anthelmintic Efficacy ◽  
Pyrantel Pamoate

The research was conducted to observe anthelmintic efficacy on rhizome extract bangle against Ascaridia galli by in vitro procedure. Experimental study was conducted, with samples rhizomes bangle powder and  maceration method in three concentrations of 2%, 4% and 8%. The experimental animals used is Ascaridia galli with NaCl 0.9% as negative control and a pyrantel pamoate suspension as positive control with concentrations of 0.2%, 0.4% and 0.6%. The study was conducted in July 2014 at the Laboratory on IKIFA Academy of Pharmacy. The data analysis used is a probit analysis. Results of Research was rhizome extract bangle (Zingiber purpureum Roxb.) having as anthelmintic efficacy against Ascaridia galli with LC50 2.29% and LT50 in 4 hours 3 minutes 18 seconds

scholarly journals Potensi Ekstrak Buah Mangrove Xylocarpus granatum Untuk Pemberantasan Larva Nyamuk Aedes aegypti

2018 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Delianis Pringgenies ◽  
Rini Widiyadmi ◽  
Ervia Yudiati ◽  
Muhammad Syaifudien Bahry ◽  
Ali Djunaedi
Keyword(s):  
Aedes Aegypti ◽  
Positive Control ◽  
Negative Control ◽  
Mosquito Larvae ◽  
Mosquito Repellent ◽  
Fruit Extract ◽  
Xylocarpus Granatum

ABSTRAK   Tujuan penelitian adalah untuk mengetahui potensi ekstrak buah mangrove Xylocarpus granatum sebagai anti larva nyamuk Aedes aegypti. Uji larva nyamuk dengan memasukkan masing-masing 10 ekor larva nyamuk yang dimasukkan ke dalam 7 botol cup tranparan kecil,  masing– masing untuk 50, 100, 250, 500, 1000 ppm sampel ektrak,  kontrol negative dan  kontrol positive. Hasil penelitian memperlihatkan bahwa pengaruh  tingkat mortalitas larva nyamuk terhadap ekstrak buah mangrove Xylocarpus granatum pada jam 1 memperlihatkan bahwa pada konsentrasi 1000 ppm, persentasi mortalitas nyamuk tertinggi adalah 81,34 %, dan pada konstrasi ekstrak buah mangrove Xylocarpus granatum 100 ppm terendah  mortalitasnya =  68,8%. Perlakuan pada jam 3 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove 500 ppm tingkat mortalitas larva nyamuk tertinggi (80%).  Pada konsentrasi ekstrasi buah mangrove 250 ppm, mortalitasnya 66,70% dan konsentrasi ekstral buah mangrove pada 100 ppm, mortalitasnya adalah 40%. Hasil peneltian pada jam ke 24 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove mencapai 1000 pmm, mortalitasnya 100%, pengamatan jam ke 48  mortalitasnya  100%. Sedang pada perlakuan control positif, keluulushidupan larva nyamuk adalah 0% dan pada control negative, kelulushidupan larva nyamuk adalah 100%. Kesimpulan:  bahwa ekstrak buah mangrove Xylocarpus granatum berpotensi sebagai anti larva nyamuk pada konsentrasi 1000 ppm.   ABSTRACT   It was assumed that mangrove fruits has some insecticidal biosubstances. Aim of the research is the potentials of mangrove Xylocarpus granatum fruit extract as the anti mosquito (Aedes aegypti) repellent. Experiment using of 10 mosquito larvae in 7 bottles each filled with 50; 100; 250; 500 and 1000 ppm fruit extract, negative  and positive control.  The experiment reveals that after 1 hour treatment, the 1000 ppm exctract had the hihgest mosquito larvae mortality of 81.34%, while the lowest extract of 100 ppm had 68.8% mortality.  After 3 hours of extract treatment the 500 ppm had the hihgest mortality of 80%, 250 ppm with 66.70% and 100 ppm with 40 % mortality.  Result of experiment after 24 as well as  48 hours treatment the 1000 ppm extract had 100% mortality.  In the positive control had 0% mortality and the negative control had 100% of survival. The summary is that the mangrove Xylocarpus granatum fruit extract had a potential as mosquito repellent at 1000 ppm fruit extract.

scholarly journals Test of Larvacide Activity from Some Sponge Extracts to Aedes aegypti Larvae

2018 ◽  
Vol 6 (2) ◽  
pp. 83
Author(s):  
Efra D. L. Wantah ◽  
R. E. P. Mangindaan ◽  
Fitje Losung
Keyword(s):  
Aedes Aegypti ◽  
Biological Activities ◽  
Positive Control ◽  
Negative Control ◽  
Clean Water ◽  
Test Results ◽  
Ethanolic Extracts ◽  
Sponge Extract ◽  
Freeze Dryer ◽  
Made In

Marine organisms have been known produce certain compounds those could lead for medicine purposes. Sponges are one and the most studied for this aim. oneof the important biological activities which expected from sponge are larvacide activity. The aims this research was to test the larvacide activity aagainst Aedes aegypti larvae from some of sponge extract. Sponge samples were taken from Malalayang Waters, (N 01 ° 27'37 "E 124 ° 47'30") on November 2014 with the depth varies from 2-15 m  with SCUBA. The extraction, preparation of the larvae and activity testing was performed on Biomolecular and Marine Pharmacy Laboratory Faculty Fisheries and Marine Science. The sponge samples were cutted and soaked in 95% Ethanol for over night with 3 repetitions to obtain ethanolic extracts. The extract were filtered and evaporated using freeze dryer then tested onto 10 instars 3 instars m larvae that had been previously maintained. the test was made in triplowith 24 hours observation. abate was used as positive control while negative control clean water was used.The test results showed that of 11 Sponge tested, 10 species showed larvacidal activity and marine sponge extract Tedania sp. has the highest activity compared to 9 extracts. As a suggestion of this research the further purification of Tedania sp. extract is needed to know the structure of active compound.Keywords: Aedes aegypti, Larvacide, Sponge extractABSTRAKOrganisme laut yang dapat dikembangkan menjadi bahan sediaan obat antara lain sponge, dan merupakan salah satu organisme laut yang banyak diteliti. Beberapa aktivitas biologis penting yang diharapkan dari ekstrak sponge salah satunya adalah aktivitas larvasida. Penelitian ini bertujuan untuk menguji aktivitas larvasida nyamuk Aedes aegypti dari beberapa ekstrak Sponge. Sampel sponge diambil di perairan Malalayang, tepatnya di koordinat N 01°27’37” E 124°47’30” pada bulan November 2014 di kedalaman 2-15 m. Sedangkan untuk tahap ekstraksi, penyiapan larva uji dan pengujian aktivitas larvasida di lakukan di laboratorium Biomolekular dan Farmasitika Laut program studi Ilmu Kelautan, FPIK UNSRAT. Dalam penelitian yang dilakukan, sampel diambil di perairan menggunakan peralatan SCUBA. Setelah itu diekstrak dengan larutan etanol 95% dan direndam selama 24 jam dan dilakukan 3 kali pengulagan untuk mendapatkan ekstrak etanolik. Sampel dikeringkan dengan menggunakan alat freeze dryer kemudian diujikan ke 10 ekor larva nyamuk fase instar 3 yang telah dipelihara sebelumnya. Pengujian dilakukan sebanyak 3 kali pengulangan dengan lama pengamatan 24 jam pengamatan. Sebagai kontrol positif digunakan bubuk abate yang biasa dijual di pasaran sedangkan kontrol negative atau tanpa perlakuan digunakan air bersih. Data hasil pengamatan diolah menggunakan Microsoft excel.Hasil pengujian menunjukkan bahwa Dari 11 Sponge yang diuji, 10 jenis menunjukan aktivitas larvasida dengan persentase mortalitas yang bervariasi dan ekstrak sponge laut Tedania sp. memiliki aktivitas tertinggi dibandingkan dengan 10 jenis ekstrak sponge lain dalam pengujian. Sebagai saran dalam penelitian ini yaitu Perlu dilakukan pemurnian lanjut ekstrak lebih lanjut dari ekstrak sponge Tedania sp. yaitu ke tahap partisi dan Perlu adanya variasi konsentrasi dalam pengujian.Kata Kunci : Aedes aegypti, Larvasida, ekstrak Spong

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