scholarly journals A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice

2021 ◽  
Vol Volume 14 ◽  
pp. 3503-3509
Author(s):  
Joshua Crawford ◽  
Sufang Liu ◽  
Feng Tao
Keyword(s):  
Real Time ◽  
Neuronal Activity ◽  
Multidisciplinary Approach ◽  
Brain Neurons ◽  
Pain Behaviors ◽  
Optogenetic Stimulation ◽  
Freely Moving ◽  
Stimulation Of

scholarly journals SkinnerTrax: high-throughput behavior-dependent optogenetic stimulation of Drosophila

2017 ◽  
Author(s):  
Ulrich Stern ◽  
Chung-Hui Yang
Keyword(s):  
High Throughput ◽  
Closed Loop ◽  
Optogenetic Stimulation ◽  
Cns Neurons ◽  
Highly Effective ◽  
Freely Moving ◽  
Stimulation Of

AbstractWhile red-shifted channelrhodopsin has been shown to be highly effective in activating CNS neurons in freely moving Drosophila, there were no existing high-throughput tools for closed-loop, behavior-dependent optogenetic stimulation of Drosophila. Here, we present SkinnerTrax to fill this void. SkinnerTrax stimulates individual flies promptly in response to their being at specific positions or performing specific actions. Importantly, SkinnerTrax was designed for and achieves significant throughput with simple and inexpensive components.

scholarly journals Modification of oxygen consumption and blood flow in mouse somatosensory cortex by cell-type-specific neuronal activity

2019 ◽  
Author(s):  
Matilda Dahlqvist ◽  
Kirsten Thomsen ◽  
Dmitry Postnov ◽  
Martin Lauritzen
Keyword(s):  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rhythmic Activity ◽  
Inhibitory Neurons ◽  
Gamma Activity ◽  
Somatosensory Stimulation ◽  
Neuronal Excitation ◽  
Optogenetic Stimulation ◽  
Higher Cognitive Functions ◽  
Stimulation Of

AbstractGamma activity arises from the interplay between pyramidal neurons and fast-spiking parvalbumin (PV) interneurons, is an integral part of higher cognitive functions and is assumed to contribute importantly to brain metabolic responses. Cerebral metabolic rate of oxygen (CMRO2) responses were evoked by optogenetic stimulation of cortical PV interneurons and pyramidal neurons. We found that CMRO2 responses depended on neuronal activation, but not on the power of gamma activity induced by optogenetic stimulation. This implies that evoked gamma activity per se is not energy demanding. Optogenetic stimulation of PV interneurons during somatosensory stimulation reduced excitatory neuronal activity but did not potentiate O2 consumption as previously hypothesized. In conclusion, our data suggest that activity-driven CMRO2 responses depend on neuronal excitation rather than the cerebral rhythmic activity they induce. Excitation of both excitatory and inhibitory neurons requires energy, but inhibition of cortical excitatory neurons by interneurons does not potentiate activity-driven energy consumption.

scholarly journals Transient effect of mossy fiber stimulation on spatial firing of CA3 neurons

2018 ◽  
Author(s):  
Joonyeup Lee ◽  
Miru Yun ◽  
Eunjae Cho ◽  
Jong Won Lee ◽  
Doyun Lee ◽  
...  
Keyword(s):  
Mossy Fiber ◽  
Empirical Test ◽  
Mossy Fibers ◽  
Transient Effect ◽  
Optogenetic Stimulation ◽  
Place Fields ◽  
Freely Moving ◽  
Ca3 Neurons ◽  
Fiber Stimulation ◽  
Stimulation Of

AbstractStrong hippocampal mossy fiber synapses are thought to function as detonators, imposing ‘teaching’ signals onto CA3 neurons during new memory formation. For an empirical test of this long-standing view, we examined effects of stimulating mossy fibers on spatial firing of CA3 neurons in freely-moving mice. We found that optogenetic stimulation of mossy fibers can alter CA3 spatial firing, but their effects are only transient. Spatially restricted mossy fiber stimulation, either congruent or incongruent with CA3 place fields, was more likely to suppress than enhance CA3 neuronal activity. Also, changes in spatial firing induced by optogenetic stimulation reverted immediately upon stimulation termination, leaving CA3 place fields unaltered. Our results do not support the traditional view that mossy fibers impose teaching signals onto CA3 network, and show robustness of established CA3 spatial representations.

scholarly journals The STROBE: a system for closed-looped optogenetic control of freely feeding flies

2018 ◽  
Author(s):  
Pierre-Yves Musso ◽  
Pierre Junca ◽  
Meghan Jelen ◽  
Damian Feldman-Kiss ◽  
Han Zhang ◽  
...  
Keyword(s):  
Sensory Neurons ◽  
Dopaminergic Neurons ◽  
Mushroom Body ◽  
Internal State ◽  
Feeding Behaviors ◽  
Optogenetic Stimulation ◽  
Freely Moving ◽  
Neural Excitation ◽  
Stimulation Of ◽  
Feeding Circuits

AbstractManipulating feeding circuits in freely moving animals is challenging, in part because the timing of sensory inputs is affected by the animal’s behavior. To address this challenge in Drosophila, we developed the Sip-Triggered Optogenetic Behavior Enclosure (“STROBE”). The STROBE is a closed-looped system for real-time optogenetic activation of feeding flies, designed to evoke neural excitation coincident with food contact. We demonstrate that optogenetic stimulation of sweet sensory neurons in the STROBE drives attraction to tasteless food, while activation of bitter sensory neurons promotes avoidance. Moreover, feeding behavior in the STROBE is modified by the fly’s internal state, as well as the presence of chemical taste ligands. We also find that mushroom body dopaminergic neurons and their respective post-synaptic partners drive opposing feeding behaviors following activation. Together, these results establish the STROBE as a new tool for dissecting fly feeding circuits and suggest a role for mushroom body circuits in processing naïve taste responses.

scholarly journals Holographic optogenetic stimulation of patterned neuronal activity for vision restoration

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Inna Reutsky-Gefen ◽  
Lior Golan ◽  
Nairouz Farah ◽  
Adi Schejter ◽  
Limor Tsur ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Optogenetic Stimulation ◽  
Vision Restoration ◽  
Stimulation Of

scholarly journals Modification of oxygen consumption and blood flow in mouse somatosensory cortex by cell-type-specific neuronal activity

2019 ◽  
Vol 40 (10) ◽  
pp. 2010-2025 ◽  
Author(s):  
Matilda K Dahlqvist ◽  
Kirsten J Thomsen ◽  
Dmitry D Postnov ◽  
Martin J Lauritzen
Keyword(s):  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rhythmic Activity ◽  
Inhibitory Neurons ◽  
Gamma Activity ◽  
Somatosensory Stimulation ◽  
Neuronal Excitation ◽  
Optogenetic Stimulation ◽  
Higher Cognitive Functions ◽  
Stimulation Of

Gamma activity arising from the interplay between pyramidal neurons and fast-spiking parvalbumin (PV) interneurons is an integral part of higher cognitive functions and is assumed to contribute significantly to brain metabolic responses. Cerebral metabolic rate of oxygen (CMRO2) responses were evoked by optogenetic stimulation of cortical PV interneurons and pyramidal neurons. We found that CMRO2 responses depended on neuronal activation, but not on the power of gamma activity induced by optogenetic stimulation. This implies that evoked gamma activity per se is not energy demanding. Optogenetic stimulation of PV interneurons during somatosensory stimulation reduced excitatory neuronal activity but did not potentiate O2 consumption as previously hypothesized. In conclusion, our data suggest that activity-driven CMRO2 responses depend on neuronal excitation rather than the cerebral rhythmic activity they induce. Excitation of both excitatory and inhibitory neurons requires energy, but inhibition of cortical excitatory neurons by interneurons does not potentiate activity-driven energy consumption.

304 REAL-TIME IMAGING OF CORTICAL NEURONAL ACTIVITY DURING THERMAL AND ELECTRICAL STIMULATION OF THE SKIN

2006 ◽  
Vol 10 (S1) ◽  
pp. S81b-S81
Author(s):  
S.F. Worthen ◽  
P. Adjamian ◽  
P.L. Furlong ◽  
Q. Aziz ◽  
B.A. Chizh ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Real Time ◽  
Neuronal Activity ◽  
Real Time Imaging ◽  
Stimulation Of

scholarly journals Optogenetic stimulation of infralimbic PFC reproduces ketamine’s rapid and sustained antidepressant actions

2015 ◽  
Vol 112 (26) ◽  
pp. 8106-8111 ◽  
Author(s):  
Manabu Fuchikami ◽  
Alexandra Thomas ◽  
Rongjian Liu ◽  
Eric S. Wohleb ◽  
Benjamin B. Land ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rodent Models ◽  
Cellular Mechanisms ◽  
Optogenetic Stimulation ◽  
Layer V ◽  
And Function ◽  
Stimulation Of ◽  
Synaptic Responses

Ketamine produces rapid and sustained antidepressant actions in depressed patients, but the precise cellular mechanisms underlying these effects have not been identified. Here we determined if modulation of neuronal activity in the infralimbic prefrontal cortex (IL-PFC) underlies the antidepressant and anxiolytic actions of ketamine. We found that neuronal inactivation of the IL-PFC completely blocked the antidepressant and anxiolytic effects of systemic ketamine in rodent models and that ketamine microinfusion into IL-PFC reproduced these behavioral actions of systemic ketamine. We also found that optogenetic stimulation of the IL-PFC produced rapid and long-lasting antidepressant and anxiolytic effects and that these effects are associated with increased number and function of spine synapses of layer V pyramidal neurons. The results demonstrate that ketamine infusions or optogenetic stimulation of IL-PFC are sufficient to produce long-lasting antidepressant behavioral and synaptic responses similar to the effects of systemic ketamine administration.

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